A new PCR-based approach for the specific amplification of DNA from different Schistosoma species applicable to human urine samples

Sandoval, Nidia; Siles-Lucas, Mar; Pérez-Arellano, José Luís; Carranza, Cristina; Puente, Sabino; López‐Abán, Julio; Muro, Antonio

doi:10.1017/s0031182006000898

Cited by 129 publications

(101 citation statements)

References 19 publications

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“…Schistosoma mansoni-specific DNA has been detected in urine 7 days p.i. in an experimental model (Sandoval et al 2006b), suggesting the possibility of excretion of free circulating pathogen DNA in urine. Similarly, other parasites, free circulating DNA was detected by PCR during early infection periods in the experimental models for Trypanosoma cruzi (Kirchhoff et al 1996), filariasis (Albers et al 2014) and Toxoplasma gondii (Weiss et al 1991;Wang et al 2012), thus allowing the diagnosis of infection earlier than when using conventional diagnostic techniques as microscopy or serology.…”

Section: Resultsmentioning

confidence: 99%

Diagnostic and prognostic value of cell free circulating Schistosoma mansoni DNA: an experimental study

Eraky

Aly

2014

J Parasit Dis

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Searching for a more sensitive and accurate marker for schistosomiasis diagnosis and treatment follow up is a potential necessity. Hereby, we evaluated usefulness of circulating free DNA as a marker for schistosomiasis diagnosis, assessing drug efficacy and monitoring the control interventions impact using SYBR green real-time PCR. A batch of mice were infected by 90 ± 10 Schistosoma mansoni cercariae. Starting from the 2nd day post infection (p.i.), groups of 2 or 3 mice were sacrificed every 3 days until 30 days p.i. The remaining animals were treated by a single dose of 400 mg/kg mefloquine and sacrificed in group at 5, 10, 21 days post treatment (35, 40, 51 days p.i.). Using SYBR green real time qPCR, pooled sera DNA were extracted and amplified. The results showed that, circulating free S. mansoni DNA was detected from the 2nd day post infection (p.i.) onwards with gradual decrease in the cycle threshold value C t which indicates the gradual elevation of the DNA level (Log quantity was 2.6-3.1 IU/ml), As the infection progressed, DNA quantity was increased(Log quantity was 6.29 IU/ml). Initial increase of circulating free DNA was observed 10 days post treatment (40 days p.i.) (Log quantity was 7.38 IU/ ml). That was followed by a progressive decrease in DNA level by the end of 21st day, post treatment (51 p.i.) (Log quantity 4.35 IU/ml). In conclusion, circulating free S. mansoni DNA is a reliable marker in the diagnosis of schistosomiasis and for assessing drug efficacy and monitoring the impact of control interventions.

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Section: Resultsmentioning

confidence: 99%

Diagnostic and prognostic value of cell free circulating Schistosoma mansoni DNA: an experimental study

Eraky

Aly

2014

J Parasit Dis

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“…Mean infection intensity was 3.0 EPG (range 0-88). Of the 11 Kato-Katz positive individuals, seven had only 1-3 eggs detected (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24). At the village level, S. japonicum infections were detected by Kato-Katz and the hatching test in two of the four villages, and by PCR in three of the four villages (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…Earlier studies have described PCR methods for detection of Schistosoma mansoni and Schistosoma haematobium DNA in animal models and in human samples. [12][13][14][15][16] Schistosoma japonicum PCR techniques have also been described. [17][18][19][20][21][22] However, the use of PCR techniques to screen field-collected samples has been limited.…”

Section: Introductionmentioning

confidence: 99%

Field Evaluation of a PCR Test for Schistosoma japonicum Egg Detection in Low-Prevalence Regions of China

Fung

Xiao²,

Wang³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Sensitive Schistosoma japonicum detection methods are needed to progress from schistosomiasis control to elimination. The sensitivity of the Kato-Katz thick smear and miracidium hatching tests decrease with infection intensity and serological tests cannot always identify current infections. We evaluated a fecal polymerase chain reaction (PCR) assay to detect S. japonicum infection in 106 humans and 8 bovines in China. PCR was highly sensitive, detecting S. japonicum DNA at 0.5 eggs/g of stool. Comparing PCR examination of a single stool sample to the miracidium hatching test using three consecutive stool samples, more humans were hatching test positive (20%) than PCR positive (15%). However, two individuals were PCR positive in a village where no infections were detected by coprological methods. The sensitivity of PCR makes it a promising tool for schistosomiasis diagnostics and screening, although egg shedding variability and stool sample size present challenges for any detection method in low-transmission areas.

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“…Using schistosome genome specific primers, allowed detection of the parasite DNA by polymerase chain reaction (PCR) in biological samples from infected subjects with superior specificity and sensitivity than the parasitological or the serological methods [7][8][9][10][11][12][13][14], as demonstrated by the capacity of the PCR to detect parasite DNA as early as 7 days post infection. While, the levels of the anti-worm IgG remained high at 23 weeks post-treatment, the PCR results turned negative at week 10 posttreatment [14].…”

Section: Introductionmentioning

confidence: 99%

Comparative sensitivities of various tests for diagnosing early Schistosoma mansoni infection in mice

Bahgat¹,

Ibrahim²,

Maghraby³

et al. 2011

J Bacteriol Parasitol

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Objective: We compared the diagnostic values of cercarial antigen preparation, cercarial secretions, soluble worm antigen preparation and worm vomit prepared from the parasite Schistosoma mansoni.Methods: Enzyme linked immunosorbant assay was used to detect IgG in plasma from Schistosoma mansoni infected mice. In parallel, specific primers for the parasite genome was used to detect S. mansoni DNA in plasma and urine from infected mice and hemolymph and tissues of infected Biomphalaria alexandrina snails by Polymerase chain reaction. Results:The results showed that all the above diagnostic approaches enabled infection to be diagnosed as early as three days post mice exposure to parasite cercariae. Conclusion:Cercarial secretions and worm vomit represent new useful economic crude antigens for preliminary detection of parasite active transmission or response to therapy in an endemic setting. Also, it was found that the detection of Schistosoma mansoni DNA in urine from infected mice was the most sensitive and specific (although expensive) method for infection diagnosis than all the others. Comparative sensitivities of various tests for diagnosing early Schistosoma mansoni infection in mice Journal of Bacteriology and ParasitologyCitation: Bahgat MM, Ibrahim AM, Maghraby AS, Rizk M, Megeed RA (2011) Comparative sensitivities of various tests for diagnosing early Schistosoma mansoni infection in mice. J Bacteriol Parasitol 2:116.

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A new PCR-based approach for the specific amplification of DNA from different Schistosoma species applicable to human urine samples

Cited by 129 publications

References 19 publications

Diagnostic and prognostic value of cell free circulating Schistosoma mansoni DNA: an experimental study

Diagnostic and prognostic value of cell free circulating Schistosoma mansoni DNA: an experimental study

Field Evaluation of a PCR Test for Schistosoma japonicum Egg Detection in Low-Prevalence Regions of China

Comparative sensitivities of various tests for diagnosing early Schistosoma mansoni infection in mice

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