A new paradigm for Aedes spp. surveillance using gravid ovipositing sticky trap and NS1 antigen test kit

Lau, Sai Ming; Chua, Tock Hing; Sulaiman, Wan Yussof; Joanne, Sylvia; Lim, Yvonne Ai Lian; Sekaran, Shamala Devi; Chinna, Karuthan; Balan, Venugopalan; Vythilingam, Indra

doi:10.1186/s13071-017-2091-y

Cited by 32 publications

(32 citation statements)

References 62 publications

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“…Commercial Dengue NS1 Ag detection assays have also been used as a tool for the surveillance of DENV in field-caught mosquitoes. This approach has demonstrated the benefits of dengue NS1 Ag rapid assays over PCR [18,32,33]. The reported detection threshold of 10 6 RNA copies in DENV-infected mosquitoes by dengue NS1 Ag rapid assays was consistent with our readings for DENV cultures.…”

Section: Discussionsupporting

confidence: 82%

Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays

et al. 2019

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Dengue virus (DENV) and Zika virus (ZIKV) are flaviviruses of public health relevance. Both viruses circulate in the same endemic settings and acute infections generally manifest similar symptoms. This highlights the importance of accurate diagnosis for clinical management and outbreak control. One of the commonly used acute diagnostic markers for flaviviruses is nonstructural protein 1 (NS1). However, false positives due to antigenic cross-reactivity have been reported between DENV and ZIKV infections when using DENV NS1 antigen (NS1 Ag) detection assays in acute cases. Therefore, we investigated the lowest detectable virus titres and cross-reactivity of three commercial dengue NS1 Ag rapid assays and two ELISAs for different flaviviruses. Our results showed that substantially high viral titres of ZIKV, Kunjin virus (KUNV) and yellow fever virus (YFV) are required to give false-positive results when using DENV NS1 rapid detection assays. Commercial DENV NS1 ELISAs did not react with ZIKV and YFV. In comparison, tested assays detected DENV at a significantly low virus titre. Given the relatively low viral loads reported in clinical samples, our findings suggest that commercially available dengue NS1 Ag detection assays are less likely to generate false-positive results among clinical samples in areas where multiple flaviviruses cocirculate.

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Section: Discussionsupporting

confidence: 82%

Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays

et al. 2019

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“…RDTs can be used as a simple method to detect DENV antigen in mosquitoes [ 77 , 78 ]. New methods to monitor DENV-infected adult Aedes densities using various trapping designs and RDTs have been proposed as a new paradigm in Aedes surveillance [ 79 , 80 ].…”

Section: Discussionmentioning

confidence: 99%

Assessing dengue transmission risk and a vector control intervention using entomological and immunological indices in Thailand: study protocol for a cluster-randomized controlled trial

Overgaard

Pientong

Thaewnongiew³

et al. 2018

Trials

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BackgroundDengue fever is the most common and widespread mosquito-borne arboviral disease in the world. There is a compelling need for cost-effective approaches and practical tools that can reliably measure real-time dengue transmission dynamics that enable more accurate and useful predictions of incidence and outbreaks. Sensitive surveillance tools do not exist today, and only a small handful of new control strategies are available. Vector control remains at the forefront for combating dengue transmission. However, the effectiveness of many current vector control interventions is fraught with inherent weaknesses. No single vector control method is effective enough to control both vector populations and disease transmission. Evaluations of novel larval and adult control interventions are needed.Methods/designA cluster-randomized controlled trial will be carried out between 2017 and 2019 in urban community clusters in Khon Kaen and Roi Et cities, northeastern Thailand. The effectiveness of a pyriproxyfen/spinosad combination treatment of permanent water storage containers will be evaluated on epidemiological and entomological outcomes, including dengue incidence, number of female adult dengue vectors infected or not infected with dengue virus (DENV), human exposure to Aedes mosquito bites, and several other indices. These indices will also be used to develop predictive models for dengue transmission and impending outbreaks. Epidemiological and entomological data will be collected continuously for 2 years, with the intervention implemented after 1 year.DiscussionThe aims of the trial are to simultaneously evaluate the efficacy of an innovative dengue vector control intervention and developing predictive dengue models. Assessment of human exposure to mosquito bites by detecting antibodies generated against Aedes saliva proteins in human blood samples has, so far, not been applied in dengue epidemiological risk assessment and disease surveillance methodologies. Likewise, DENV detection in mosquitoes (adult and immature stages) has not been used in any practical way for routine disease surveillance strategies. The integration of multiple outcome measures will assist health authorities to better predict outbreaks for planning and applying focal and timely interventions. The trial outcomes will not only be important for Thailand, but also for the entire Southeast Asian region and further afield.Trial registrationISRCTN, ISRCTN73606171. Registered on 23 June 2017.Electronic supplementary materialThe online version of this article (10.1186/s13063-018-2490-1) contains supplementary material, which is available to authorized users.

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“…Moreover, the pan-DENV RT-iiPCR can work on both systems to detect the four DENV serotypes in DENVinfected mosquitos (Table 4). Timely on-site detection of DENV in human and mosquito can potentially alert front-line health professionals to allow timely implementation of intervention strategies and help focusing efforts in targeted areas to help mitigate disease outbreaks [1,38]. Performance of such tests near POC/PON could help shift health service more to local levels, achieve disease diagnosis at early stages, and reduce costs and turn-around time, improving health management quality in underreached communities.…”

Section: Discussionmentioning

confidence: 99%

A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos

Tsai¹,

Liu²,

Lin³

et al. 2019

PLoS ONE

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Background The insulated isothermal PCR (iiPCR) technology enables consistent PCR amplification and detection in a simple heating device. A pan-dengue virus (DENV) RT-iiPCR, targeting the 5’ untranslated region, was validated previously on the semi-automated POCKIT combo system (involving separate devices for nucleic acid extraction and PCR amplification/detection) to offer performance comparable to a laboratory real-time PCR. Working on the same technologies, a compact automated sample-in-answer-out system (POCKIT Central Nucleic Acid Analyser) has been available commercially for iiPCR, minimizing human error risks and allowing easy molecular bio-detection near points of need. Here, we evaluated the analytical and clinical performance of the pan-DENV RT-iiPCR on the fully automated system by comparison to those on the semi-automated system. Methodology/Principal findings Testing sera containing serial diluted DENV-1, -2, -3, or -4 cell culture stock, the pan-DENV RT-iiPCR system had similar 100% detection endpoints on the two systems; i.e. at 1, 10, 1 and 10 PFU/ml, respectively, on the fully automated system, and at 10, 1, 10 and 10 PFU/ml, respectively, on the semi-automated system. Furthermore, both fully automated and semi-automated PCR system can detect all four DENV serotypes in mosquitos. Clinical performance of the reagent on the two systems was evaluated by testing 60 human serum samples. Both systems detected the same 40 samples (ten DENV-1, -2, -3, and -4 positive each) and did not detect the other 20; 100% agreement (κ = 1) was found between the two systems. Conclusions/Significance With performance comparable to a previously validated system, the fully-automated PCR system allows applications of the pan-DENV reagent as a useful tool near points of need to facilitate easy, fast and effective detection of dengue virus and help mitigate versatile public health challenges in the control and management of dengue disease.

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A new paradigm for Aedes spp. surveillance using gravid ovipositing sticky trap and NS1 antigen test kit

Cited by 32 publications

References 62 publications

Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays

Flavivirus Cross-Reactivity to Dengue Nonstructural Protein 1 Antigen Detection Assays

Assessing dengue transmission risk and a vector control intervention using entomological and immunological indices in Thailand: study protocol for a cluster-randomized controlled trial

A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos

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