A new optimization strategy for MALDI FTICR MS tissue analysis for untargeted metabolomics using experimental design and data modeling

Ferey, Justine; Marguet, Florent; Laquerrière, Annie; Marret, Stéphane; Schmitz-Afonso, Isabelle; Bekri, Soumeya; Afonso, Carlos; Tebani, Abdellah

doi:10.1007/s00216-019-01863-6

Cited by 18 publications

(27 citation statements)

References 43 publications

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“…Fresh frozen 10-µm sections of human muscle were mounted on conductive ITO-coated glass slides 75 × 25 mm (Bruker, Bremen, Germany) and stored at -80 °C until analysis. Matrix solutions were applied with an automatic microsprayer HTX TM-Sprayer (HTX Imaging, Chapel Hill, NC, USA) as previously described 49 . In the positive-ion mode, DHB matrix (30 mg mL -1 in MeOH/H 2 O 50:50 [v/v]) was deposited with the following parameters: nozzle temperature 80 °C, nozzle velocity 1200 mm min -1 , N 2 pressure 10 psi, N 2 flow rate 2 L min -1 , number of passes 12, flow rate 100 µL min -1 and track spacing 3 mm.…”

Section: Methodsmentioning

confidence: 99%

Muscle metabolic remodelling patterns in Duchenne muscular dystrophy revealed by ultra-high-resolution mass spectrometry imaging

Dabaj

Ferey

Marguet

et al. 2021

Sci Rep

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Duchenne muscular dystrophy (DMD) is a common and severe X-linked myopathy, characterized by muscle degeneration due to altered or absent dystrophin. DMD has no effective cure, and the underlying molecular mechanisms remain incompletely understood. The aim of this study is to investigate the metabolic changes in DMD using mass spectrometry-based imaging. Nine human muscle biopsies from DMD patients and nine muscle biopsies from control individuals were subjected to untargeted MSI using matrix-assisted laser desorption/ionization Fourier-transform ion cyclotron resonance mass spectrometry. Both univariate and pattern recognition techniques have been used for data analysis. This study revealed significant changes in 34 keys metabolites. Seven metabolites were decreased in the Duchenne biopsies compared to control biopsies including adenosine triphosphate, and glycerophosphocholine. The other 27 metabolites were increased in the Duchenne biopsies, including sphingomyelin, phosphatidylcholines, phosphatidic acids and phosphatidylserines. Most of these dysregulated metabolites are tightly related to energy and phospholipid metabolism. This study revealed a deep metabolic remodelling in phospholipids and energy metabolism in DMD. This systems-based approach enabled exploring the metabolism in DMD in an unprecedented holistic and unbiased manner with hypothesis-free strategies.

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Section: Methodsmentioning

confidence: 99%

Muscle metabolic remodelling patterns in Duchenne muscular dystrophy revealed by ultra-high-resolution mass spectrometry imaging

Dabaj

Ferey

Marguet

et al. 2021

Sci Rep

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“…Thus, employing our approach for future work will be faster and completely unbiased. Combining with other experimental optimization approaches, like that of ion transmission voltage optimization 21 and/or automated data acquisition optimization, 22 our approach using RSM for MALDI matrix application optimization can be valuable for attaining the most ideal spatial and chemical information from future MALDI-MSI analyses.…”

Section: Discussionmentioning

confidence: 99%

“…For example, in MALDI untargeted metabolomics analyses, this approach has been used for optimizing automated data acquisition parameters or ion transmission voltages and laser ablation parameters. 21,22 Here, we developed an experimental approach for optimizing MALDI matrix spraying conditions to minimize analyte delocalization and simultaneously maximize the number of detected lipids in MALDI-FTICR-MSI analysis of frozen human kidney biopsies. By testing 32 combinations of MALDI matrix spraying parameters with an automated spraying device, we were able to model, using RSM, the effect each of the spraying parameters had on the delocalization of endogenous molecules and the ability to detect and annotate lipid species.…”

Section: Introductionmentioning

confidence: 99%

Response Surface Methodology As a New Approach for Finding Optimal MALDI Matrix Spraying Parameters for Mass Spectrometry Imaging

Veličković

Zhang²,

Bezbradica

et al. 2020

J. Am. Soc. Mass Spectrom.

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Supporting InformationThe Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/jasms.9b00074.Step-by-step protocol and important notes for optimizing the HTX TM-spraying parameters using the RSM, containing (Table S1) coded and actual values of factors used in the experimental design, (Figure S1) organization of tissue biopsy sections on slides together with HTX TM-sprayer conditions used for each section, (Figure S2) example METASPACE webpage that shows the uploading process, (Figure S3) example METASPACE webpage that displays putative molecular annotations and corresponding ion images, and (Figure S4) examples of endogenous and off-tissue annotations by METASPACE (PDF)The authors declare no competing financial interest.

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“…Before each batch of analyses, external calibration was performed by sodium formate infusion via an ESI source. Lock mass calibration was performed at m/z 273.039364 (matrix ion 15 ) with FT MS control software (Bruker Daltonics, Bremen, Germany). Each spectrum encompassed 500 laser shots: laser focus, medium; laser power, 45%; laser spot, < 60 µm.…”

Section: Methodsmentioning

confidence: 99%

Metabolomic analysis and mass spectrometry imaging after neonatal stroke and cell therapies in mouse brains

Tanaka

Ogawa

Fujii

et al. 2020

Sci Rep

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Ischemic brain injury provokes complex, time-dependent downstream pathways that ultimately lead to cell death. We aimed to demonstrate the levels of a wide range of metabolites in brain lysates and their on-tissue distribution following neonatal stroke and cell therapies. Postnatal day 12 mice underwent middle cerebral artery occlusion (MCAO) and were administered 1 × 105 cells after 48 h. Metabolomic analysis of the injured hemisphere demonstrated that a variety of amino acids were significantly increased and that tricarboxylic acid cycle intermediates and some related amino acids, such as glutamate, were decreased. With the exception of the changes in citric acid, neither mesenchymal stem/stromal cells nor CD34+ cells ameliorated these changes. On-tissue visualization with matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) imaging revealed that the signal intensity of glutamate was significantly decreased in the infarct area, consistent with the metabolomic analysis, while its intensity was significantly increased in the peri-infarct area after MCAO. Although cell therapies did not ameliorate the changes in metabolites in the infarct area, mesenchymal stem cells ameliorated the increased levels of glutamate and carnitine in the peri-infarct area. MALDI-MS imaging showed the location-specific effect of cell therapies even in this subacute setting after MCAO. These methodologies may be useful for further investigation of possible treatments for ischemic brain injury.

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A new optimization strategy for MALDI FTICR MS tissue analysis for untargeted metabolomics using experimental design and data modeling

Cited by 18 publications

References 43 publications

Muscle metabolic remodelling patterns in Duchenne muscular dystrophy revealed by ultra-high-resolution mass spectrometry imaging

Muscle metabolic remodelling patterns in Duchenne muscular dystrophy revealed by ultra-high-resolution mass spectrometry imaging

Response Surface Methodology As a New Approach for Finding Optimal MALDI Matrix Spraying Parameters for Mass Spectrometry Imaging

Metabolomic analysis and mass spectrometry imaging after neonatal stroke and cell therapies in mouse brains

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