A new multiplex polymerase chain reaction assay for the identification a panel of bacteria involved in bacteremia

Fazzeli, Hossein; Arabestani, Mohammad Reza; Esfahani, Bahram Nasr; Khorvash, Farzin; Pourshafie, Mohammad Reza; Moghim, Sharareh; Safaei, Hajieh Ghasemian; Faghri, Jamshid; Azimian, Amir

doi:10.4103/2277-9175.107972

Cited by 8 publications

(1 citation statement)

References 22 publications

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“…There are three procedures for applying NAT assays in infectious disease diagnosis: (1) Pathogen-specific methods, (2) multiplex assays covering several different pathogens typical for a certain infection type, and (3) using universal broad-range assays involving conserved target sequences, such as the eubacterial 16S or 23S rDNA/RNA, and the pan fungal 8S or 18S rDNA/RNA. [1139]…”

Section: Identification By Microbial Nucleic-acid Technologymentioning

confidence: 99%

Conventional, molecular methods and biomarkers molecules in detection of septicemia

et al. 2015

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Sepsis is a leading cause of morbidity and mortality in hospitalized patients worldwide and based on studies, 30–40% of all cases of severe sepsis and septic shock results from the blood stream infections (BSIs). Identifying of the disease, performing laboratory tests, and consequently treatment are factors that required for optimum management of BSIs. In addition, applying precise and immediate identification of the etiologic agent is a prerequisite for specific antibiotic therapy of pathogen and thereby decreasing mortality rates. The diagnosis of sepsis is difficult because clinical signs of sepsis often overlap with other noninfectious cases of systemic inflammation. BSIs are usually diagnosed by performing a series of techniques such as blood cultures, polymerase chain reaction-based methods, and biomarkers of sepsis. Extremely time-consuming even to take up to several days is a major limitation of conventional methods. In addition, yielding false-negative results due to fastidious and slow-growing microorganisms and also in case of antibiotic pretreated samples are other limitations. In comparison, molecular methods are capable of examining a blood sample obtained from suspicious patient with BSI and gave the all required information to prescribing antimicrobial therapy for detected bacterial or fungal infections immediately. Because of an emergency of sepsis, new methods are being developed. In this review, we discussed about the most important sepsis diagnostic methods and numbered the advantage and disadvantage of the methods in detail.

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Section: Identification By Microbial Nucleic-acid Technologymentioning

confidence: 99%

Conventional, molecular methods and biomarkers molecules in detection of septicemia

et al. 2015

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Microbiological comparison of blood culture and amplification of 16S rDNA methods in combination with DGGE for detection of neonatal sepsis in blood samples

et al. 2017

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Our study shows that the molecular approach with 16S rDNA-DGGE identifies twofold more pathogenic bacteria than bacteriological culture, including complex bacterial communities associated with the development of bacterial sepsis in neonates. What is Known: • Neonatal sepsis affects 2.3% of birth in the NICU with a high mortality risk. • Evidence supports the use of molecular methods as an alternative to blood culture for identification of bacterial associated neonatal sepsis. What is New: • The DGGE gel is a good methodological approach for the identification of bacterial in neonatal blood samples. • This study describes the pattern of electrophoretic mobility obtained by DGGE gels and allows to determine the type of bacteria associated in the development of neonatal sepsis.

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A Multiplex Nested PCR for the Simultaneous Detection of Salmonella typhi, Mycobacterium tuberculosis, and Burkholderia pseudomallei in Patients with Pyrexia of Unknown Origin (PUO) in Vellore, South India

et al. 2014

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A new multiplex polymerase chain reaction assay for the identification a panel of bacteria involved in bacteremia

Cited by 8 publications

References 22 publications

Conventional, molecular methods and biomarkers molecules in detection of septicemia

Conventional, molecular methods and biomarkers molecules in detection of septicemia

Microbiological comparison of blood culture and amplification of 16S rDNA methods in combination with DGGE for detection of neonatal sepsis in blood samples

A Multiplex Nested PCR for the Simultaneous Detection of Salmonella typhi, Mycobacterium tuberculosis, and Burkholderia pseudomallei in Patients with Pyrexia of Unknown Origin (PUO) in Vellore, South India

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