A new multiplex PCR test for the determination of Vrn-B1 alleles in bread wheat (Triticum aestivum L.)

Milec, Zbyněk; Tomková, Lenka; Sumíková, T.; Pánková, Kateřina

doi:10.1007/s11032-011-9621-7

Cited by 57 publications

(41 citation statements)

References 18 publications

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“…This spring allele has been transferred, and is now also present in triticale genotypes, however we did not show the influence of this allele on flowering time. Lately few more Vrn-B1 alleles have been described in wheat (Milec et al, 2012;Shcherban et al, 2012). Their presence can be a source of allelic variation of VRN-1 in the triticale genome as well.…”

Section: Discussionmentioning

confidence: 99%

Analysis of VRN1 gene in triticale and common wheat genetic background

Nowak

Leśniowska-Nowak

Zapalska

et al. 2014

Sci. agric. (Piracicaba, Braz.)

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In cereals, the transition from the vegetative stage to flowering is controlled in the main by the set of vernalization genes. Within these genes the most important role is played by VRN1, which encodes a MADS-box transcription factor, regulating the transition of shoot apical meristem to the reproductive phase. The level of vernalization requirement is strongly linked to the molecular structure of this gene. In this study we analyzed molecular mechanisms regulating the vernalization requirement in triticale on the basis of comparative analysis of the VRN1 locus between triticale (×Triticosecale Witt.) and common wheat (Triticum aestivum L.) genotypes. We also estimated the influence of VRN genotype on heading time and the winter hardiness of these two species. Molecular markers developed for VRN genotype detection in common wheat were successfully applied to an analysis of triticale genomic DNA. Subsequent analysis of the amplicons nucleotide sequence confirmed full similarity of the products obtained between triticale and common wheat. All winter triticale cultivars tested contained the recessive vrn-A1 allele, whereas all spring genotypes carried the dominant Vrn-A1a allele. Molecular analysis of the Vrn-B1 gene revealed the presence of the dominant Vrn-B1b allele in only one of the triticale genotypes analyzed (Legalo). The major system of determination of the vernalization requirement in triticale was transferred from common wheat without changes and is based on an alteration in the VRN1 gene promoter sequence within the A genome.

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Section: Discussionmentioning

confidence: 99%

Analysis of VRN1 gene in triticale and common wheat genetic background

Nowak

Leśniowska-Nowak

Zapalska

et al. 2014

Sci. agric. (Piracicaba, Braz.)

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“…DNA isolation and purification were carried out with the Genomic DNA Purification Kit (Termo Scientific, Lithuania) in accordance with manufacturer's protocol. To esti mate the allelic composition markers at the loci, Vrn 1 and Vrn B3 were used [3,10,13,15]. Alleles of the genes Vrn A1 and Vrn B3 were identified by PCR with dominant and codominant primer pairs.…”

Section: Methodsmentioning

confidence: 99%

“…The allele Vrn A1a is characterized by insertion in the promotor region; Vrn A1b is characterized by a 20 bp deletion in the promotor region; and Vrn A1c is characterized by deletion in the first intron [1,10]. Locus Vrn B1 is known to possess three dominant alleles: Vrn B1a is characterized by a large deletion of 6850 bp in the first intron [13]; Vrn B1b differs from Vrn B1a by a single nucleotide substitution and a 36 bp deletion in the first intron [12]; Vrn B1c is characterized by a 820 bp deletion and a duplication of 431 bp, which is trans ferred into the beginning of this deletion in the first intron [14,15].…”

Section: Introductionmentioning

confidence: 99%

Allelic composition in the Vrn-A1, Vrn-B1, and Vrn-B3 genes of double haploid lines of hexaploid triticale

Zaitseva

Лемеш

2015

Russ J Genet

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Vernalization genes are associated with the adaptation capability, heading dates, and yield poten tial of grain crops. The allelic composition in the Vrn A1, Vrn B1, and Vrn B3 genes was defined in 42 lines of double haploids of hexaploid triticale, which were produced through in vitro anther culture. Two alleles (Vrn A1a and vrn A1) were found at the Vrn A1 locus and three alleles (Vrn B1a, Vrn B1c, and vrn B1) were found at the Vrn B1 locus. All double haploids carried the recessive allele at the Vrn B3 locus. Twelve lines of spring triticale were selected, and they were characterized by an allelic composition associated with early maturity and high potential of grain yield.

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“…The reaction mixture contained 1.5 µl of 10 × PCR buffer containing MgCl 2 , 1.5 µl of 2 mM dNTP mixture, 1.5 µl of 2 µM primers (total volume for each primer), 0.07 µl of Taq DNA polymerase (5 U/µl) (all Roche Diagnostics GmbH, Mannheim, Germany), and 100-150 ng of template DNA. For the analysis of Ppd-A1, Ppd-D1, and the presence of the Vrn-A1, Vrn-B1, and Vrn-D1 alleles, protocols from Fu et al (2005), Beales et al (2007), Milec et al (2012), and Nishida et al (2012) were used. The alleles were differentiated according to fragment length and dominant markers.…”

Section: Cultivarsmentioning

confidence: 99%

Length of prematurity period in wheat cultivars determines maximum cereal aphid abundance

Trávníčková¹,

Pánková²,

Martinková³

et al. 2016

Plant Prot. Sci.

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Trávníčková M., Pánková K., Martinková Z., Honěk A. (2016): Length of prematurity period in wheat cultivars determines maximum cereal aphid abundance. Plant Protect. Sci., 52: 254-261.Maximum aphid numbers on wheat are positively related to the length of the period elapsed from immigration to the population peak. We predicted that maximum abundances on late maturing cultivars would be greater than on early ones. This was tested using 8 spring wheat cultivars that differed in the length of time to senescence. In a 4-year experiment, numbers of aphids were checked at weekly intervals. Maximum abundances on late-maturing cultivars were significantly greater than those on early cultivars. However, the length of the vegetation period affected maximum abundances less than did the annual variation in aphid abundance. Genetic disposition for early ripening thus tends to decrease aphid numbers, but manipulation of this character is unlikely to become an important source of aphid resistance.

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A new multiplex PCR test for the determination of Vrn-B1 alleles in bread wheat (Triticum aestivum L.)

Cited by 57 publications

References 18 publications

Analysis of VRN1 gene in triticale and common wheat genetic background

Analysis of VRN1 gene in triticale and common wheat genetic background

Allelic composition in the Vrn-A1, Vrn-B1, and Vrn-B3 genes of double haploid lines of hexaploid triticale

Length of prematurity period in wheat cultivars determines maximum cereal aphid abundance

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