A new multilayered membrane for tissue engineering of oral hard- and soft tissue by means of melt electrospinning writing and film casting – An in vitro study

Fuchs, Andreas; Youssef, Almoatazbellah; Seher, Axel; Hartmann, Stefan; Brands, Roman C.; Müller‐Richter, Urs; Kübler, Alexander C.; Linz, Christian

doi:10.1016/j.jcms.2019.01.043

Cited by 26 publications

(7 citation statements)

References 45 publications

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“…[15,16] Importantly, while larger strand spacing impacts cell survival, smaller spacing better supports cell viability and proliferation. [15][16][17][18] Our findings agree with previous studies, where cell proliferation was greatly influenced by pore size. [15,19] Noteworthy, at day 14, proliferation decreased in the 500 and 750 μm groups, possibly due to growth impairment due to cell-cell contact.…”

Section: Fabrication and Characterization Of Mew Pcl Scaffoldssupporting

confidence: 92%

A Highly Ordered, Nanostructured Fluorinated CaP‐Coated Melt Electrowritten Scaffold for Periodontal Tissue Regeneration

Daghrery

Ferreira

Araújo

et al. 2021

Adv Healthcare Materials

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Periodontitis is a chronic inflammatory, bacteria-triggered disorder affecting nearly half of American adults. Although some level of tissue regeneration is realized, its low success in complex cases demands superior strategies to amplify regenerative capacity. Herein, highly ordered scaffolds are engineered via Melt ElectroWriting (MEW), and the effects of strand spacing, as well as the presence of a nanostructured fluorinated calcium phosphate (F/CaP) coating on the adhesion/proliferation, and osteogenic differentiation of human-derived periodontal ligament stem cells, are investigated. Upon initial cell-scaffold interaction screening aimed at defining the most suitable design, MEW poly(𝝐-caprolactone) scaffolds with 500 μm strand spacing are chosen. Following an alkali treatment, scaffolds are immersed in a pre-established solution to allow for coating formation. The presence of a nanostructured F/CaP coating leads to a marked upregulation of osteogenic genes and attenuated bacterial growth. In vivo findings confirm that the F/CaP-coated scaffolds are biocompatible and lead to periodontal regeneration when implanted in a rat mandibular periodontal fenestration defect model. In aggregate, it is considered that this work can contribute to the development of personalized scaffolds capable of enabling tissue-specific differentiation of progenitor cells, and thus guide simultaneous and coordinated regeneration of soft and hard periodontal tissues, while providing antimicrobial protection.

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Section: Fabrication and Characterization Of Mew Pcl Scaffoldssupporting

confidence: 92%

A Highly Ordered, Nanostructured Fluorinated CaP‐Coated Melt Electrowritten Scaffold for Periodontal Tissue Regeneration

Daghrery

Ferreira

Araújo

et al. 2021

Adv Healthcare Materials

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“…PCL scaffolds and core membranes demonstrated good cytocompatibility for all cell lines, which even enhanced by fusing both components together. 88 Wei (2019) developed a gelatin collagen, PCL skin substitute using two different GC:PCL ratios (1:8 and 1:20). In vitro test results showed better cell proliferation in the scaffold with a lower collagen content.…”

Section: Cellular Bioactivity On Pcl Scaffoldsmentioning

confidence: 99%

Polycaprolactone as biomaterial for bone scaffolds: Review of literature

Dwivedi

Kumar

Pandey

et al. 2020

Journal of Oral Biology and Craniofacial Research

424

290

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Bone tissue engineering using polymer based scaffolds have been studied a lot in last decades. Considering the qualities of all the polymers desired to be used as scaffolds, Polycaprolactone (PCL) polyester apart from being biocompatible and biodegradable qualifies to an appreciable level due its easy availability, cost efficacy and suitability for modification. Its adjustable physio-chemical state, biological properties and mechanical strength renders it to withstand physical, chemical and mechanical, insults without significant loss of its properties. This review aims to critically analyse the efficacy of PCL as a biomaterial for bone scaffolds. 1.1. Polymers as biomaterial for scaffolds The characteristics of a biomaterial that must be scrutinized thoroughly before considering it for bone tissue engineering applications includes chemical composition, biological and structural characteristics, degradation behavior and fabrication process. Polymeric scaffolds are of considerable interest due to their distinctive features, such

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“…In another study, melt electrowritten structures with different geometries were designed for attachment of osteoblasts on one side, and keratinocytes and connective tissues on the other side. The fibrous structures were combined with a core PCL film that was prepared via casting to prevent possible infections for applications of oral and maxillofacial surgery [124]. In this study, the design of construct was found cytocompatible with improved cell proliferation and showed bacteria-tightness over two weeks.…”

Section: Other Hybrid Approaches With Mewmentioning

confidence: 92%

Biomimicry in Bio-Manufacturing: Developments in Melt Electrospinning Writing Technology Towards Hybrid Biomanufacturing

et al. 2019

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Melt electrospinning writing has been emerged as a promising technique in the field of tissue engineering, with the capability of fabricating controllable and highly ordered complex three-dimensional geometries from a wide range of polymers. This three-dimensional (3D) printing method can be used to fabricate scaffolds biomimicking extracellular matrix of replaced tissue with the required mechanical properties. However, controlled and homogeneous cell attachment on melt electrospun fibers is a challenge. The combination of melt electrospinning writing with other tissue engineering approaches, called hybrid biomanufacturing, has introduced new perspectives and increased its potential applications in tissue engineering. In this review, principles and key parameters, challenges, and opportunities of melt electrospinning writing, and particularly, recent approaches and materials in this field are introduced. Subsequently, hybrid biomanufacturing strategies are presented for improved biological and mechanical properties of the manufactured porous structures. An overview of the possible hybrid setups and applications, future perspective of hybrid processes, guidelines, and opportunities in different areas of tissue/organ engineering are also highlighted.

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A new multilayered membrane for tissue engineering of oral hard- and soft tissue by means of melt electrospinning writing and film casting – An in vitro study

Cited by 26 publications

References 45 publications

A Highly Ordered, Nanostructured Fluorinated CaP‐Coated Melt Electrowritten Scaffold for Periodontal Tissue Regeneration

A Highly Ordered, Nanostructured Fluorinated CaP‐Coated Melt Electrowritten Scaffold for Periodontal Tissue Regeneration

Polycaprolactone as biomaterial for bone scaffolds: Review of literature

Biomimicry in Bio-Manufacturing: Developments in Melt Electrospinning Writing Technology Towards Hybrid Biomanufacturing

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