A new multi-host species indirect ELISA using protein A/G conjugate for detection of anti-Toxoplasma gondii IgG antibodies with comparison to ELISA-IgG, agglutination assay and Western blot

Al-Adhami, Batol; Gajadhar, Alvin A.

doi:10.1016/j.vetpar.2013.11.004

Cited by 37 publications

(28 citation statements)

References 22 publications

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“…As protein A/G can strongly bind to the IgG of many mammalian species, the use of a protein A/G conjugate in ELISA has been demonstrated to be useful for the diagnosis of infectious diseases in multiple species. 1 In the present study, we investigated the level of anti-T. gondii IgG in farmed mink in northeast China using the MAT, WB, and 3 ELISAs with protein A/G conjugate and recombinant dense granule antigen 1 (GRA1), GRA7, and Toxoplasma soluble antigens (TSA). The WB was selected as the reference standard for this evaluation study as it has high specificity and sensitivity.…”

Section: Brief Communicationmentioning

confidence: 99%

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A comparative study of Toxoplasma gondii seroprevalence in mink using a modified agglutination test, a Western blot, and enzyme-linked immunosorbent assays

Wang

Cai

et al. 2015

J VET Diagn Invest

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Toxoplasma gondii can infect almost all warm-blooded animals, and many serological methods have been developed to detect T. gondii infection in a variety of animal species. In the present study, the seroprevalence of T. gondii infection in farmed mink in northeast China was determined using the modified agglutination test (MAT), a Western blot (WB), and 3 enzyme-linked immunosorbent assays (ELISAs) with protein A/G conjugate, using either of 2 recombinant dense granule antigens, GRA1 and GRA7, or Toxoplasma soluble antigens (TSA). There was no significant difference between the detection results of the GRA1-, GRA7-, and TSA-ELISAs and WB (McNemar chi-square, P > 0.05), but a significant difference was observed between MAT and WB (P < 0.05). A near perfect agreement (97.0%) was found between the GRA7-ELISA and WB (κ = 0.83), and a substantial agreement (92.4-93.1%) was observed in the TSA- and GRA1-ELISAs (κ = 0.68-0.73). The GRA7-ELISA showed the highest sensitivity and specificity, and the lowest false-positive and negative rates, while the MAT gave both a low sensitivity and frequent false positives in comparison to the WB. Receiver operating characteristic analysis revealed the largest area under curve of 0.85 (95% confidence interval: 0.74-0.96), and the highest relative sensitivity (72.7%) and specificity (99.0%) for a cutoff value of 0.19 in the GRA7-ELISA. These results indicate that the GRA7-ELISA is suitable for detection of T. gondii infection in mink and that MAT should be used with caution.

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Section: Brief Communicationmentioning

confidence: 99%

“…The WB was selected as the reference standard for this evaluation study as it has high specificity and sensitivity. 1 The MAT was conducted as described previously. 7 Briefly, each serum sample was diluted in a 2-fold series from 1:25 to 1:200 in 96-well, U-bottom microtiter plates.…”

Section: Brief Communicationmentioning

confidence: 99%

A comparative study of Toxoplasma gondii seroprevalence in mink using a modified agglutination test, a Western blot, and enzyme-linked immunosorbent assays

Wang

Cai

et al. 2015

J VET Diagn Invest

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“…Since rodents are among the most species-rich groups, it is impractical to have specific conjugates for each genus or species. Thus, in this study an immunoassay to detect anti- T. gondii antibodies in different species of rodents was developed, using as conjugate protein A coupled to peroxidase [1]. It was adequate for all species tested except for Peromyscus spp.…”

Section: Discussionmentioning

confidence: 99%

Toxoplasmosis seroprevalence in wild small rodents, potentially preys of ocelots in north-eastern Mexico

et al. 2014

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The aim of this study was to assess the prevalence of Toxoplasma gondii infection in rodents that coexist with ocelots in north-eastern Mexico. Eighty rodents of five genera were captured and their serum samples tested for specific IgG antibodies to T. gondii by in-house indirect ELISA using three different conjugates. Prevalences of 7% (3/44) and 33% (4/12) were found in Sigmodon hispidus and Liomys irroratus, respectively, and were significantly different. All Baiomys taylori and Oligoryzomys fulvescens were negative for the presence of anti-T. gondii IgG antibodies. The samples from Peromyscus spp. could not be analyzed because none of the three conjugates tested recognized their immunoglobulins. Infection was confirmed in one single specimen of L. irroratus by qPCR, which generated an estimate of 146 parasites per mg of muscle tissue. The results strongly support the notion of active T. gondii transmission between rodents and felines in this zone of Mexico and an important role of some rodent species in the sylvatic cycle of T. gondii.

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“…When implemented for humans, surveillance often relies on voluntary reporting and follow-up on presumptive cases only, or targeting high-risk populations rather than the general population at large (Alvarado-Esquivel et al, 2014). A recently developed indirect ELISA using a nonspecies-specific protein A/G conjugate has proved effective for the detection of T. gondii in a variety of mammalian hosts, including wildlife (Al-Adhami and Gajadhar, 2014). Similarly, the numerous surveillance studies of livestock, wildlife, or foods for these parasites typically have been on a relatively small scale.…”

Section: Coccidian Protozoamentioning

confidence: 99%

Components of control for foodborne parasites and their application in the food production chain

Scandrett

2015

Foodborne Parasites in the Food Supply Web

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A new multi-host species indirect ELISA using protein A/G conjugate for detection of anti-Toxoplasma gondii IgG antibodies with comparison to ELISA-IgG, agglutination assay and Western blot

Cited by 37 publications

References 22 publications

A comparative study of Toxoplasma gondii seroprevalence in mink using a modified agglutination test, a Western blot, and enzyme-linked immunosorbent assays

A comparative study of Toxoplasma gondii seroprevalence in mink using a modified agglutination test, a Western blot, and enzyme-linked immunosorbent assays

Toxoplasmosis seroprevalence in wild small rodents, potentially preys of ocelots in north-eastern Mexico

Components of control for foodborne parasites and their application in the food production chain

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