A new model of transient complete obstruction of retinal vessels induced by endothelin-1 injection into the posterior vitreous body in rabbits

Takei, Kohtaro; Sato, Tsuyoshi; Nonoyama, Tomohito; Miyauchi, Takashi; Goto, Katsutoshi; Hommura, Sachiko

doi:10.1007/bf02044235

Cited by 58 publications

(36 citation statements)

References 18 publications

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“…The retinal blood supply in rhesus monkeys [16,17] is similar to that in human; however, the sources of these animals are limited, and they are difficult to breed and manage. Compared with the eyeballs of other animal species, the rabbit eyeball is sufficiently large to operate [19][20][21][22][23] . Further, rabbits are inexpensive, can be easily bred and the FFA of the rabbit eye is stable.…”

Section: Discussionmentioning

confidence: 99%

“…In intravitreous thrombin instillation method [19][20][21] , the location of the retinal thrombus could not be determined; thus, the thrombus may occlude other regions but not the target vessels. The injection of endothelin-I into the posterior vitreous body [22,23] caused complete occlusion of the temporal retinal vessels, but this vasospastic mechanism is also unsuitable for application in the experimental induction of thrombosis because actual clinical BRVO was occluded at one or some spots in vein.…”

Section: Discussionmentioning

confidence: 99%

“…The pathological features of BRVO can be more accurately reproduced by a photodynamic method to induce thrombosis in retinal vessels [4][5][6][7][8][9][10][11][12][13][14] than by using other methods such as laser coagulation [15][16][17] , electrocoagulation of the retinal vein via the vitreous body [18] , intravenous thrombin instillation [19][20][21] and endothelin-I injection into the posterior vitreous body [22,23] . In the photodynamic method, a photosensitizing agent, namely, rose Bengal, is injected intravenously.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Induction of Branch Retinal Vein Occlusion by Photodynamic Therapy with Rose Bengal in a Rabbit Model

Zhou¹,

Song²,

Zhao³

et al. 2012

Advances in Ophthalmology

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Induction of Branch Retinal Vein Occlusion by Photodynamic Therapy with Rose Bengal in a Rabbit Model

Zhou¹,

Song²,

Zhao³

et al. 2012

Advances in Ophthalmology

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“…In a model of absolute transitory retinal ischaemia validated by Takei et al (1993), intravitreal injections of ET-1 were performed in rabbit eyes and the analysis of the ECGs proved that there was an absolute transitory retinal ischaemia for at least 50 min and no choroidal ischaemia.…”

Section: Discussionmentioning

confidence: 99%

Endothelin‐1 effects on spontaneous oscillations in choroidal arterioles

Delgado¹,

Marques-Neves²,

Rocha³

et al. 2009

Acta Ophthalmologica

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ABSTRACT.Purpose: This study characterizes the effects of endothelin-1 (ET-1) on the perfusion pressure of the choroidal vasculature using in situ perfused isolated rabbit eyes. Methods: Rabbit external ophthalmic arteries (n = 12) in a head-mounted preparation were cannulated and perfused with warmed tyrode. The three-way polypropylene catheter was further connected to a pressure transducer and the effect of intraluminal pressure as a measure of total vascular resistance was assessed. Response curves to intra-arterial injections of ET-1 (group A; n = 6) and to an intravitreal injection followed by an intra-arterial injection of ET-1 (group B; n = 6) were obtained. Data were studied using paired t-test and fast Fourier transform. Results: Before any drugs were administered, spontaneous oscillations were observed in the 12 rabbit models. In group A, ET-1 induced a short and weak vasodilating effect followed by a strong and long-lasting vasoconstrictor tone. Vasomotion became more evident, showing a higher frequency and shorter amplitude of oscillations. In group, B the intravitreal injection produced no significant changes in registered pressure or vasomotion characteristics. The intra-arterial injection produced effects similar to those observed in group A. Conclusion: Our study has three main findings: (i) the choroidal vasculature demonstrated spontaneous oscillations in perfusion pressure in basal conditions in all rabbit eye models; (ii) ET-1 applied intra-arterially induced a short drop in perfusion pressure followed by a long withstanding contraction; and (iii) intra-arterial ET-1 modulated the frequency and amplitude of the spontaneous oscillations, causing a faster rate of pulsatility.

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“…Some methods including mechanical ligation, endothelin-1 injection and light coagulation for RVO animal models in rats, cats, or rabbits have been defined. However, laser photocoagulation with a photo sensitizer is currently the most common method to induce RVO in rats [171][172][173][174]. Recently, another reproducible and reliable animal RVO model has been developed in rats and miniature pigs.…”

Section: Retinal Vein Occlusionmentioning

confidence: 99%

Experimental Animal Models for Retinal and Choroidal Diseases

Turgut

Karanfil

2017

AOVS

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Experimental animal models have critical importance for the recognition and the management of various retinal and choroidal diseases. To date, they were described for the induction of melanoma and neovascularization of choroid, degenerations, and detachment in the retina, proliferative vitreoretinopathy, diabetic and hypertensive retinopathy, pre-retinal neovascularization, central serous chorioretinopathy, retinopathy of prematurity (ROP) or O 2 induced retinopathy (OIR) and retinoblastoma. The aim of this review is to provide an overview of experimental animal models for retinal and choroidal diseases. To know the most favorite models and animals for these diseases will provide many utilities to the researchers in their experimental studies and thesis. Keywords: Experimental Animal Models for Retinal and Choroidal Diseases 2/10Copyright: ©2017 Turgut et al.primate laser-induced CNV models in rhesus and cynomolgus monkeys have been choiced for preclinical evaluation of n-AMD treatment options [19,21,22,26]. Except for the LFC induced CNV, the sub-retinal NV has been formed in transgenic mice through the increased expression of vascular endothelial growth factor in the retina [23]. Retinal Detachment (RD)The animal RD models are important in the evaluation of the pathogenesis of the disease and new treatment methods in . Many studies have used the mouse as a model to study retinal detachment/reattachment. Mouse eye is a good model for the experimental eye diseases because its manupilation is easy [41][42][43][44]. In animal RD models, manual peeling off the neuroretina from the retina pigment epithelium (RPE) and the subretinal injection of saline, balanced salt solution, phosphatebuffered saline (PBS), hyaluronate or RPE cells have been used to establish an experimental RD in rodent eyes and provide the pathologic features of human . The subretinal injection of sodium hyaluronate has some advantages such as no known ocular toxicity and the long-duration of RD compared to others. The subretinal injection of sodium hyaluronate may performed with a trans-vitreal or a trans-scleral way with the observation of the fundus [28,29,[32][33][34]40]. In the trans-vitreal method, a sub-retinal injector is introduced into the vitreous cavity, a peripheral retinotomy is created, and the sodium hyaluronate is injected into the subretinal space. In this method, two retinal tears are created to increase the risk of retinal hemorrhage. Subretinal hemorrhage or hemorrhage entered underneath the retina is toxic for photoreceptor cells through retinal hypoxia and metabolic impairment due toits diffusion barrier effect, and toxicity due to iron in blood in the detached retina. This induces retinal degeneration and RD [28,29,[32][33][34]40].In trans-scleral method, a 30 G needle with a syringe containing sodium hyaluronate is inserted into the subretinal space through the conjunctiva, sclera, choroid, and RPE following reduction of intraocular pressure (IOP) with an anterior chamber parasenthesis. The sodium hyaluronate is ...

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A new model of transient complete obstruction of retinal vessels induced by endothelin-1 injection into the posterior vitreous body in rabbits

Cited by 58 publications

References 18 publications

Induction of Branch Retinal Vein Occlusion by Photodynamic Therapy with Rose Bengal in a Rabbit Model

Induction of Branch Retinal Vein Occlusion by Photodynamic Therapy with Rose Bengal in a Rabbit Model

Endothelin‐1 effects on spontaneous oscillations in choroidal arterioles

Experimental Animal Models for Retinal and Choroidal Diseases

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