A New Model of Experimental Prosthetic Joint Infection Due to Methicillin-Resistant Staphylococcus aureus: A Microbiologic, Histopathologic, and Magnetic Resonance Imaging Characterization

Belmatoug, Nadia; Crémieux, Anne‐Claude; Bleton, R.; Volk, Andreas; Saleh‐Mghir, Azzam; Grossin, M; Garry, Louis; Carbon, C

doi:10.1093/infdis/174.2.414

Cited by 80 publications

(46 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Histologic examination included assessment of morphologic features and semiquantitative grading (19,20). For each joint, the following elements were analyzed to determine the intensity of inflammatory lesions: joint cavity (normal or dilated, presence or absence of liquid and/or cells), synovial membrane (normal or abnormal, edema, synovial hyperplasia, vascular congestion, cellular infiltration and/or fibrosis, type of inflammatory cells), articular surfaces (normal or presence of chondral and/or bone destruction and/or fibrosis), and tendon sheaths (normal or synovial hyperplasia, edema, vascular congestion, cellular infiltration, and fibrosis).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Vitamin E uncouples joint destruction and clinical inflammation in a transgenic mouse model of rheumatoid arthritis

Bandt

Grossin

Driss³

et al. 2002

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective. Reactive oxygen species are thought to play a role in rheumatoid arthritis (RA) in humans. We postulated that antioxidant treatment could have a beneficial effect in this disease. We therefore investigated the effects of vitamin E in the transgenic KRN/ NOD mouse model of RA.Methods. Mice were treated by gavage with oral vitamin E (␣-tocopherol). Clinical, histologic, and biochemical parameters were assessed for 6 weeks.Results. Vitamin E treatment did not modify the clinical features of the disease (date of onset or disease intensity, as measured by the articular index), but it did prevent joint destruction, as measured by qualitative and semiquantitative analyses. Redox status did not differ between treated and control mice. White blood cell chemiluminescence was higher in transgenic KRN/NOD mice than in controls, but vitamin E treatment attenuated this difference. Vitamin E treatment of the transgenic animals led to a significant decrease in the levels of interleukin-1␤ (IL-1␤) but not tumor necrosis factor ␣.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Regarding transgenic animals, results were as follows: Histologic scoring of the lesions (19,20) was performed on mice treated at the scheduled dose of vitamin E (3 mice in each group). Mice were given vitamin E or placebo treatment and killed on days 60, 66, 70, 90, 100, or 120 (i.e., from the end of treatment until 2 months later).…”

mentioning

confidence: 99%

Vitamin E uncouples joint destruction and clinical inflammation in a transgenic mouse model of rheumatoid arthritis

Bandt

Grossin

Driss³

et al. 2002

Arthritis & Rheumatism

View full text Add to dashboard Cite

show abstract

“…Most of skeletal implant infection studies are based upon perioperative introduction of bacteria to the implant bed, demonstrating the relationship between S. aureus and metal 35,36 or silicone-elastomer 26 implant infection, as well as the distal spread of periprosthetic infection reproducing progressive chronic osteomyelitis. Nevertheless, they do not address the clinical problem of hematogenous implant infections.…”

Section: Discussionmentioning

confidence: 99%

“…The animals were obtained from a professional stock breeder, and have been proven suitable for the investigation of osteomyelitis. [26][27][28] All animals were preoperatively evaluated by a veterinary doctor through physical examination (body weight, rectal temperature), complete white blood cell (WBC) count, and erythrocyte sedimentation rate (ESR) to rule out infection. Normal values for this type of animals are as follows: WBC (5.2-12.5 Â 10 3 /ml), 29 ESR (0-20 mm/h), 30 temperature (388-398C), 29 and weight (2.9-3.6 kg) 29 for approximately 4-monthold rabbits (skeletally mature) which were used in this study.…”

Section: Methods Study Model and Animalsmentioning

confidence: 99%

Novel model for studying hematogenous infection in an experimental setting of implant‐related infection by a community‐acquired methicillin‐resistant S. aureus Strain

Poultsides

Papatheodorou

Karachalios

et al. 2008

Journal Orthopaedic Research

View full text Add to dashboard Cite

The aim of this study is to establish a new experimental model of hematogenous implant-related infection (IRI) by a communityacquired methicillin-resistant S. aureus (CA-MRSA) strain. Cylindrical porous tantalum intramedullary implants were inserted in the proximal right tibia of 30 male white rabbits after administration of antibiotic prophylaxis. Four weeks later and without antibiotic prophylaxis, 20 animals received 1 ml of inoculum of two different concentrations (study groups A and B) of CA-MRSA strain through an ipsilatelar femoral artery catheter. The remaining 10 received normal saline instead (control group C). Surviving animals were sacrificed 4 weeks later. Sterile bone, bone marrow biopsies, and implants were harvested for culture and histological evaluation. Ten animals receiving 5 Â 10 8 cfu/ml (group A) died within 48-72 h due to septic shock. Blood cultures were positive; histology demonstrated acute infection. Ten animals received bacterial load of 3 Â 10 8 cfu/ml (group B) and all survived; two had negative Gram-stain and cultures but PCR and RT-PCR results demonstrated the viability of the microorganisms, while periprosthetic osteolysis and histological evaluation indicated subacute osteomyelitis; eight animals established periprosthetic infection, osteomyelitis, and septic arthritis documented by positive Gram-stain, cultures, subperiosteal reaction, and chronic infection on histology. Control group specimens demonstrated no signs of infection. Histopathological semiquantitative scoring was used to compare the three groups. Comparison of groups A and B with control group and between group A and B showed statistically significant difference ( p < 0.05) in all parameters except for periosteal reaction between groups B and C ( p ¼ 0.354). This novel, reproducible experimental model will facilitate the study of hematogenous CA-MRSA IRIs. ß

show abstract

“…Causative organism streptococcus aureus adhere to bone and expressing receptors for bone matrix. An insignificant skin infection emerge the serious infection such as sub-acute and acute bacterial endocarditic [7]. In children and adults, osteomyelitis mainly involves metaphysis of long bone, femur, proximal radius and hummers [8,9].…”

Section: Pathogenesismentioning

confidence: 99%

Osteomyelitis: Identification and Management

Kumari¹

2016

MOJOR

View full text Add to dashboard Cite

ObjectiveOsteomyelitis is a severe inflammation within the bone, bone marrow and surrounding soft tissue, that develops secondary to infection with microbial organisms. The objective of this article is to present an over view regarding the identification and management of osteomyelitis. IntroductionOsteomyelitis is acute and chronic inflammatory process based upon the histopathological finding rather than duration of infection. Osteomyelitis occurring primarily in children and adolescents pathogenic micro-organism cause inflammation and infection of bone, which lead to osteomyelitis and signs appears after two weeks of infection (Figure 1) In chronic osteomyelitis necrotic bone is present and signs may not appear until six weeks of infection [1]. Osteomyelitis infection is based on open wound, haematogenous or direct bacterial inoculation inside bone [2]. Radio nuclides imagine have improved diagnostic accuracy and better orthopaedic techniques as well as use of some prophylactic regimens which help to minimize the risk of infection among osteomyelitis patient. EtiologyBacterial Staphylococcus is common infection which causes an acute and chronic haematogenous osteomyelitis. Different types of pathogens among children are Type A streptococcus, Streptococcus pneumonia, Kingella kingae and Type B streptococcal infection is mainly occur in infants [3]. Streptococcus aureus is the common pathogen in bone among adults. Progressively, methicillinresistant Staphylococcus aureus is separated from patients with osteomyelitis [4]. Other persistent cases may be caused by adjacent infection, Pseudomonas aeruginosa, Escherichia coli and Staphylococcus epidermidis. Other reported cases of osteomyelitis are mycobacterial infections and fungal, these are rare and usually found in patients with weakened of body immunity [5]. PathogenesisOsteomyelitis may be occurring direct inoculation of causative organism in bone. Normal bone is highly resistant to infection occur in case of injury, trauma, and existence of foreign bodies [6]. Causative organism streptococcus aureus adhere to bone and expressing receptors for bone matrix. An insignificant skin infection emerge the serious infection such as sub-acute and acute bacterial endocarditic [7]. In children and adults, osteomyelitis mainly involves metaphysis of long bone, femur, proximal radius and hummers [8,9]. Penetrating injury and surgical contamination are the most common causes of direct inoculation osteomyelitis. Patent with severe vascular diseases often lead to osteomyelitis. Clinical ManifestationOsteomyelitis of acute haematogenous results from bacterial growth of bone. Most of the Children are more prone to infection because of developing of meta-physeal regions of the long bones, rich in blood supply and more prone to slight trauma. Acute haematogenous osteomyelitis in children occur about one-half of all patients who are under five years [10]. Children usually came with the symptom of exist fever, irritability, local erythema, swelling in soft tissues and tenderness bo...

show abstract

A New Model of Experimental Prosthetic Joint Infection Due to Methicillin-Resistant Staphylococcus aureus: A Microbiologic, Histopathologic, and Magnetic Resonance Imaging Characterization

Cited by 80 publications

References 8 publications

Vitamin E uncouples joint destruction and clinical inflammation in a transgenic mouse model of rheumatoid arthritis

Vitamin E uncouples joint destruction and clinical inflammation in a transgenic mouse model of rheumatoid arthritis

Novel model for studying hematogenous infection in an experimental setting of implant‐related infection by a community‐acquired methicillin‐resistant S. aureus Strain

Osteomyelitis: Identification and Management

Contact Info

Product

Resources

About