A New Mirandea (Acanthaceae) from Nuevo Leon, Mexico

Daniel, Thomas F.

doi:10.2307/2418755

Cited by 2 publications

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“…The Acanthaceae is a notably eurypalynous family (Raj, 1961) and all palynological studies of the family remark on the pollen diversity. Consequently many members of the Acanthaceae have been the subject of detailed pollen morphological studies and a number of different pollen types have been identified for the family (e.g., Lindau, 1895; Bremekamp, 1953; Raj, 1961; Daniel 1978, 1986, 1990, 1998; Scotland, 1990, 1992, 1993; Perveen & Qaiser, 2010). Pollen morphology is one of the characteristics which have been used extensively to fine tune most existing classifications of the family Acanthaceae, especially at the genus level (e.g., Lindau, 1895; Bremekamp, 1953, 1965; Balkwill & Getliffe Norris, 1988; Immelman, 1989; McDade et al, 2000; Scotland & Vollesen, 2000).…”

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confidence: 99%

FIB-SEM: An Additional Technique for Investigating Internal Structure of Pollen Walls

House

Balkwill

2013

Microsc Microanal

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Pollen grain morphology has been widely used in the classification of the Acanthaceae, where external pollen wall features have proved useful in determining relationships between taxa. Although detailed information has been accumulated using light microscopy, transmission electron microscopy and scanning electron microscopy (SEM) techniques, internal pollen wall features lack investigation and the techniques are cumbersome. A new technique involving precise cross sectioning or slicing of pollen grains at a selected position for examining wall ultrastructure, using a focused ion beam-scanning electron microscope (FIB-SEM), has been explored and promising results have been obtained. The FIB-SEM offers a good technique for reliable, high resolution, three-dimensional (3D) viewing of the internal structure of the pollen grain wall.

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