A New Microbial Contact Assay for Marine Sediments (7 pp)

Heise, Susanne; Ahlf, Wolfgang

doi:10.1065/jss2005.02.131

Cited by 35 publications

(15 citation statements)

References 19 publications

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“…In the United States and Canada, macroinvertebrates have mostly been used for whole sediment toxicity testing [10][11][12]; standardized sediment contact tests (SCTs) with organisms from other trophic levels did not exist until recently. However, within the last two decades, new SCTs have been developed in Europe using a broader range of organisms, such as bacteria [13][14][15], yeast [16], nematodes [17,18], fish embryos [19,20], and macrophytes [21], with some of them being standardized (ISO 10872; ISO/DIS 16191; ISO/DIS 10871) and approved for assessing the toxicity of contaminated freshwater sediments [22,23].…”

Section: Introductionmentioning

confidence: 99%

Sediment contact tests as a tool for the assessment of sediment quality in German waters

Feiler

Höss

Ahlf

et al. 2012

Enviro Toxic and Chemistry

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Abstract-A sediment contact test (SCT) battery consisting of five ecotoxicological test systems was applied to 21 native freshwater sediments characterized by a broad variety of geochemical properties and anthropogenic contamination. Higher plants (Myriophyllum aquaticum), nematodes (Caenorhabditis elegans), oligochaetes (Lumbriculus variegatus), zebrafish embryos (Danio rerio), and bacteria (Arthrobacter globiformis), representing various trophic levels and exposure pathways, were used as test organisms. The test battery detected sediment toxicity caused by anthropogenic pollution, whereas the various tests provided site-specific, nonredundant information to the overall toxicity assessment. Based on the toxicity pattern derived from the test battery, the sediments were classified according to a newly proposed classification system for sediment toxicity assessment. The SCT-derived classification generally agreed well with the application of consensus-based sediment quality guidelines (SQGs), especially with regard to sediments with high toxic potential. For sediments with low to medium toxic potential, the SQGs often underestimated the toxicity that was detected by the SCTs, underpinning the need for toxicity tests in sediment quality assessment. Environ. Toxicol. Chem. 2013;32:144-155. # 2012 SETAC

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Section: Introductionmentioning

confidence: 99%

Sediment contact tests as a tool for the assessment of sediment quality in German waters

Feiler

Höss

Ahlf

et al. 2012

Enviro Toxic and Chemistry

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“…(Table 2) elucidates novel methods and reviews. For example, the mostly cited paper "A new microbial contact assay for marine sediments" by Heise and Ahlf (2005) has been cited 19 times. Beside the impact factor, the journal immediacy index is worth mentioning, since this index "can provide a useful perspective for comparing journals specializing in cuttingedge research" (Thomson 2008), indicating how quickly articles are cited.…”

Section: Self Citationsmentioning

confidence: 99%

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2008

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“…Chem. 25,2006 T. Kosmehl et al of cellular enzymes eventually leading to DNA alteration and fragmentation. Since the median tail moment varied only insignificantly between one and four and since, for reasons of animal care, the number of fish embryos should be kept to a minimum, eight embryos were selected as optimum for the standard protocol.…”

Section: Effects Of the Number Of Embryos On Mechanically Induced Dnamentioning

confidence: 99%

“…Although sample processing and extraction are suspected to discount bioavailability [19], organic extracts of whole sediments have been used both to assess the potential ecotoxicological burden of sediments [20][21][22] and in situations that require identification of soluble toxicants via toxicity identification evaluation procedures [20,23]. However, native whole-sediment exposure protocols represent a more realistic scenario to simulate in situ exposure conditions [24][25][26]. Therefore, the present study attempts to utilize whole-sediment exposure scenarios to mimic normal bioavailability of toxicants.…”

Section: Introductionmentioning

confidence: 99%

A novel contact assay for testing genotoxicity of chemicals and whole sediments in zebrafish embryos

Kosmehl

Hallare

Reifferscheid

et al. 2006

Enviro Toxic and Chemistry

110

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Broad consensus exists that whole-sediment exposure protocols represent the most realistic scenario to simulate in situ exposure conditions. So far, however, several endpoints including genotoxicity in vertebrate-based systems could be tested only after transfer of particle-bound substances into the aqueous phase. The present study was carried out to develop a protocol for generating a suspension of single cells from sediment-exposed zebrafish embryos that is suitable for detecting particle-bound genotoxicity in the alkaline single cell gel electrophoresis (comet assay). In this solid-phase genotoxicity assay, a whole-body cell suspension derived from zebrafish embryos exposed to native (whole) sediments is assayed in the comet assay. Several chemical and mechanical isolation procedures were compared to optimize cell yield and minimize DNA damage by the method itself. If compared to collagenase isolation, mechanical cell dissociation gave less DNA damage; trypsinization resulted in similarly low DNA damage but significantly lower cell yield. In order to test the optimized protocol, effects of well-known genotoxicants (4-nitroquinoline-N-oxide, nitrofurantoin, hydrogen peroxide, benzo[a]pyrene) and of two sediments from the upper Rhine River (Germany) on zebrafish embryos were investigated. Results documented clear-cut genotoxicity for all four substances and for one of the two whole-sediment samples. An ultraviolet (UV) light exposure of whole embryos and primary cultures from embryos elucidated only minor effects for the whole embryos compared to the primary cells. Consequently, UV irradiation cannot be suggested as a positive control in intact zebrafish embryos. In conclusion, the newly developed sediment contact assay can be recommended for the detection of both single substances but also the bioavailable fraction of the total hazard potential of sediments.

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A New Microbial Contact Assay for Marine Sediments (7 pp)

Cited by 35 publications

References 19 publications

Sediment contact tests as a tool for the assessment of sediment quality in German waters

Sediment contact tests as a tool for the assessment of sediment quality in German waters

The first impact factor

A novel contact assay for testing genotoxicity of chemicals and whole sediments in zebrafish embryos

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