A new micro-fluid chip calorimeter for biochemical applications

Lerchner, J.; Wolf, Antje; Wolf, G.; Baier, V.; Kessler, E.; Nietzsch, M.; Krügel, M.

doi:10.1016/j.tca.2005.07.011

Cited by 82 publications

(53 citation statements)

References 8 publications

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“…There are mainly two temperature sensing methods in calorimetry: Thermopile and thermistor. Thermopiles (thermocouples connected in series) are used as thermometers in most MEMS DSCs [55][56][57]. The thermocouple converts the temperature difference to electric voltage via thermoelectricity.…”

Section: Temperature Sensing and Heating Unitmentioning

confidence: 99%

Review of MEMS differential scanning calorimetry for biomolecular study

Wang

et al. 2017

Front. Mech. Eng.

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Differential scanning calorimetry (DSC) is one of the few techniques that allow direct determination of enthalpy values for binding reactions and conformational transitions in biomolecules. It provides the thermodynamics information of the biomolecules which consists of Gibbs free energy, enthalpy and entropy in a straightforward manner that enables deep understanding of the structure function relationship in biomolecules such as the folding/unfolding of protein and DNA, and ligand bindings. This review provides an up to date overview of the applications of DSC in biomolecular study such as the bovine serum albumin denaturation study, the relationship between the melting point of lysozyme and the scanning rate. We also introduce the recent advances of the development of micro-electro-mechanic-system (MEMS) based DSCs.

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Section: Temperature Sensing and Heating Unitmentioning

confidence: 99%

Review of MEMS differential scanning calorimetry for biomolecular study

Wang

et al. 2017

Front. Mech. Eng.

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“…For these applications, the precision of the temperature stabilization is particularly critical for obtaining a good signal resolution [32]. A miniaturized flow-through calorimeter was therefore installed in a sophisticated thermostat system, providing a temperature stability of better than 40 μK [10], [13], [16], [33]- [36]. More recently, such system was combined with the so-called segmented flow technology, which consists in repetitive sequential processing of sample segments separated by immiscible inert segments [37], [38].…”

Section: Introductionmentioning

confidence: 99%

Nanocalorimetric platform for accurate thermochemical studies in microliter volumes

et al. 2015

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A straightforward and general way for monitoring chemical reactions is via their thermal signature. Such approach requires however an experimental setup with a high thermal stability that simultaneously allows time-resolved heat detection with high sensitivity. We present a nanocalorimetric platform for accurate thermochemical studies of (bio-)chemical reactions in a miniaturized format (tens of microliter volume), characterized by a fast thermalization time to a preset temperature (< 30 minutes), an excellent base temperature stability (± 1 mK) and a fast sensing response time (few seconds). The platform is built around a commercial thermopile-based sensor chip, on which an open-well reservoir holding the sample is directly positioned. The sample is, prior to the experiment, pipetted into the reservoir, in which small aliquots of reagents are injected subsequently and sequentially via thermalized microfluidic conducts. The design of the platform is optimized by means of numerical simulations. Via thermoelectric calibration using a resistive heater positioned either on the sensor chip or in the reservoir, we obtain a maximum power sensitivity of 2.7 V/W and a heat limit of detection of 70 nW. The excellent functionality of the platform is demonstrated by measuring the reaction enthalpy of 1-propanol in water and the rate constant k and enthalpy change of the oxidation reaction of glucose catalyzed by glucose oxidase, showing good agreement with literature data. Our versatile platform may be applied to many thermochemical studies, including thermodynamic analysis and kinetic reaction analysis, and its ease of use will allow implementation of many different experimental protocols.

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“…The thin film encapsulating structures, in contrast to other bulky closed chamber configuration l-calorimeters, which are of the order of millimeters in thickness. 7,11,12 As a result, the thermal mass of the fabricated reaction chamber is about 3 orders of magnitude smaller compared to the conventional bulky chambers, directly resulting in a corresponding increase in temperature detection sensitivity. In this device, the heater and the temperature sensor are integrated on different sides of the reaction chamber such that the sample can be placed between the heater and the sensor.…”

Section: Introductionmentioning

confidence: 99%

A novel on-chip three-dimensional micromachined calorimeter with fully enclosed and suspended thin-film chamber for thermal characterization of liquid samples

et al. 2014

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A microfabricated calorimeter (l-calorimeter) with an enclosed reaction chamber is presented. The 3D micromachined reaction chamber is capable of analyzing liquid samples with volume of 200 nl. The thin film low-stress silicon nitride membrane is used to reduce thermal mass of the calorimeter and increase the sensitivity of system. The l-calorimeter has been designed to perform DC and AC calorimetry, thermal wave analysis, and differential scanning calorimetry. The l-calorimeter fabricated with an integrated heater and a temperature sensor on opposite sides of the reaction chamber allows to perform thermal diffusivity and specific heat measurements on liquid samples with same device. Measurement results for diffusivity and heat capacitance using time delay method and thermal wave analysis are presented. V C 2014 AIP Publishing LLC. [http://dx

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A new micro-fluid chip calorimeter for biochemical applications

Cited by 82 publications

References 8 publications

Review of MEMS differential scanning calorimetry for biomolecular study

Review of MEMS differential scanning calorimetry for biomolecular study

Nanocalorimetric platform for accurate thermochemical studies in microliter volumes

A novel on-chip three-dimensional micromachined calorimeter with fully enclosed and suspended thin-film chamber for thermal characterization of liquid samples

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