A new methodology for processing mouthparts of hemipterans (Hemiptera) for microscopic studies

Depieri, Rogério A.; Andrade, Carlos Rogério; Panizzi, Antônio R.

doi:10.1016/j.micron.2009.09.006

Cited by 4 publications

(1 citation statement)

References 5 publications

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“…Although most result in adequate quality for basic descriptive goals, 10 , 14 , 22 there is room for improvement in the systematic production of histologic slides adequate for diagnostic purposes. Many of the techniques described suffered from brittleness, 8 shrinkage, 12 , 17 a high level of complexity, or required the use of embedding media, such as resin, which cannot be processed with conventional histotechnology equipment. 1 , 3 , 5 Our aim was to develop a tissue fixation and processing method enabling histologic examination of bee samples under light microscopy, with the ultimate goal of characterizing normal tissue appearance and, eventually, tissue modifications as a consequence of pathogens, intoxication, or other conditions.…”

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confidence: 99%

Histology of the whole body of honey bees: tissue fixation and processing

Lussier

Racine²,

Benoit‐Biancamano³

2023

J VET Diagn Invest

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Beekeeping plays a crucial role in biodiversity, pollination, commercial farming, and the worldwide agricultural economy. Histopathology, which is an important tool for the investigation of diseases in vertebrates, is not commonly used in honey bees ( Apis mellifera). However, histopathology could potentially help the diagnostic investigation of high mortality in bees. We developed a tissue fixation and processing method enabling systematic production of histologic slides adequate for diagnostic and research purposes. Our method uses inexpensive, accessible products and can be realized with conventional pathology laboratory equipment. The quality of histologic slides obtained is similar to those of vertebrate animals processed routinely in pathology laboratories. Histopathology as a diagnostic and research tool will improve the services currently offered to apiarists and could help decrease the mean mortality rate, increase apiarists’ profits, and ensure long-term pollination services.

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mentioning

confidence: 99%

Histology of the whole body of honey bees: tissue fixation and processing

Lussier

Racine²,

Benoit‐Biancamano³

2023

J VET Diagn Invest

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Preparation of genitalia, mouthparts and other body parts

2014

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Measuring proboscis length in Lepidoptera: a review

2020

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Mouthpart morphologies relate to diet range. Differences among or within species may result in resource partitioning and speciation. In plant-pollinator interactions, mouthpart length has an important role in foraging efficiency, resource partitioning and pollination, hence measuring nectarivorous insect mouthparts’ morphological variation is important. Most adult lepidopterans feed on nectars and participate in pollination. Although a vast range of studies applied morphometric measurements on lepidopteran proboscis (tongue) length, general recommendations on methodologies are scarce. We review available proboscis length measurement methodologies for Lepidoptera. Focusing on how proboscides have been measured, how accurate the measurements were, and how were these constrained by sampling effort, we searched for research articles investigating lepidopteran proboscis length and extracted variables on the aims of measurements, preparation and measurement methodology, and descriptive statistics. Different methods were used both for preparation and measurements. Many of the 135 reviewed papers did not provide descriptions of the procedures applied. Research aims were different among studies. Forty-four percent of the studies measured dead specimens, 13% measured living specimens, and 43% were unclear. Fifteen percent of the studies used callipers, 9% rulers, 1% millimetre scales, 4% ocular micrometers, 3% drawings and 14% photographs; 55% were non-informative. We emphasise the importance to provide detailed descriptions on the methods applied. Providing guidelines for future sampling and measurements, we encourage fellow researchers planning measurements to take into account the effect of specimen preparation techniques on the results, define landmarks, consider resolution, accuracy, precision, choose an appropriate sample size and report details on methodology.

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A new methodology for processing mouthparts of hemipterans (Hemiptera) for microscopic studies

Cited by 4 publications

References 5 publications

Histology of the whole body of honey bees: tissue fixation and processing

Histology of the whole body of honey bees: tissue fixation and processing

Preparation of genitalia, mouthparts and other body parts

Measuring proboscis length in Lepidoptera: a review

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