A new methodology for plant cell viability assessment using intracellular esterase activity

Steward, Nicolas; Martin, Richard J.; Engasser, Jean‐Marc; Goergen, Jean‐Louis

doi:10.1007/s002990050729

Cited by 52 publications

(32 citation statements)

References 30 publications

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“…The root-apex TZ, as expected from the findings of Sivaguru and Horst (1998) in maize, proved to be the perception site of Al toxicity in Arabidopsis as well, as demonstrated by the staining pattern of propidium iodide (PI), which accumulates only in dying and dead cells, and fluorescein diacetate (FDA) staining, which is taken up and converted into the green fluorescent compound "fluorescin" only by living cells (Supplemental Figure 1; Moore et al, 1998;Steward et al, 1999).…”

Section: Resultsmentioning

confidence: 75%

TAA1-Regulated Local Auxin Biosynthesis in the Root-Apex Transition Zone Mediates the Aluminum-Induced Inhibition of Root Growth inArabidopsis

et al. 2014

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The transition zone (TZ) of the root apex is the perception site of Al toxicity. Here, we show that exposure of Arabidopsis thaliana roots to Al induces a localized enhancement of auxin signaling in the root-apex TZ that is dependent on TAA1, which encodes a Trp aminotransferase and regulates auxin biosynthesis. TAA1 is specifically upregulated in the root-apex TZ in response to Al treatment, thus mediating local auxin biosynthesis and inhibition of root growth. The TAA1-regulated local auxin biosynthesis in the root-apex TZ in response to Al stress is dependent on ethylene, as revealed by manipulating ethylene homeostasis via the precursor of ethylene biosynthesis 1-aminocyclopropane-1-carboxylic acid, the inhibitor of ethylene biosynthesis aminoethoxyvinylglycine, or mutant analysis. In response to Al stress, ethylene signaling locally upregulates TAA1 expression and thus auxin responses in the TZ and results in auxin-regulated root growth inhibition through a number of auxin response factors (ARFs). In particular, ARF10 and ARF16 are important in the regulation of cell wall modification-related genes. Our study suggests a mechanism underlying how environmental cues affect root growth plasticity through influencing local auxin biosynthesis and signaling.

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Section: Resultsmentioning

confidence: 75%

TAA1-Regulated Local Auxin Biosynthesis in the Root-Apex Transition Zone Mediates the Aluminum-Induced Inhibition of Root Growth inArabidopsis

et al. 2014

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“…The FDA staining indicates the esterase activity by the multiple esterase enzymes in the viable cell. In this context, the fluorescence of FDA staining does not verify the metabolism activity of the cell (Steward et al, 1999a(Steward et al, , 1999b. We should limit our conclusion to the esterase activity of the Nostoc sp.…”

Section: Revitalization and Recovery Of The Growth Of Nostoc Sp Hk-0mentioning

confidence: 88%

Growth of terrestrial cyanobacterium, Nostoc sp., on Martian Regolith Simulant and its vacuum tolerance

et al. 2008

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The growth of terrestrial cyanobacterium, Nostoc sp., on the Martian Regolith Simulant (MRS) and its vacuum tolerance were studied as one of our challenges in this century to inhabit Mars. The viability of the tested cyanobacteria was evaluated by microscopic observation after staining by fluorescein diacetate (FDA). The general terrestrial cyanobacterial lump collected from the ground showed a significantly high tolerance to a high vacuum environment (10 -5 Pa) for over one year. To scientifically elucidate its high tolerance function, Nostoc sp. HK-01 was used as another suitable scientific material for this study. After exposure to the high vacuum environment (10 -5 Pa) for two weeks, Nostoc sp. HK-01 began to grow again. Some of it was also re-incubated again with a liquid culture medium. The A'MED (Arai's Mars Ecosystem Dome) is designed to be installed on Mars for agricultural production. A'MED was useful to conduct our study. We performed the fundamental experiment using the MRS. Nostoc sp. HK-01 was found to grow for over 140 days along with having the normal function of chlorophyll synthesis on the MRS. These results show the possibility that cyanobacteria could adapt to the MRS, and grow under the low pressure environment expected on Mars.

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“…The FDA staining indicates the esterase activity by the multiple esterase enzymes in the viable cell. In this context, the fluorescence of FDA staining does not verify the metabolism activity of the cell (Steward et al, 1999a(Steward et al, , 1999b. I should limit our conclusion to the esterase activity of the Nostoc sp.…”

Section: Revitalization and Recovery Of The Growth Of Nostoc Sphk-01mentioning

confidence: 87%

Cyanobacteria for Space Agriculture on Mars

Arai

2009

Biol. Sci. Space

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The growth of terrestrial cyanobacterium, Nostoc sp., on the Martian Regolith Simulant (MRS) and its vacuum tolerance were examined in relevance of our challenges to inhabit M a r s . T h e via b ilit y of t h e cyanob a c te ria was evaluated by microscopic observation a f t e r s t a i n i n g b y f l u o r e s c e i n d i a c e t a t e (FDA). The general terrestrial cyanobacterial lump collected from the ground showed a significantly high tolerance against exposure to high vacuum environment for more than one year. To elucidate its high tolerance function scientifically, Nostoc sp. HK-01 was used as another material for this study. After two weeks exposure to high vacuum environment (10 -5 Pa), Nostoc sp. HK-01 start to grow again in a liquid culture medium. The A'MED (Arai's Mars Ecosystem Dome) is designed to be installed on Mars for agricultural production. A'MED was found useful to conduct our study. Nostoc sp. HK-01 grew for over 140 days, and chlorophyll synthesis was normal on the MRS. These results verify the possible adaptation of cyanobacteria, and growth under the low atmospheric pressure environment on Mars.

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A new methodology for plant cell viability assessment using intracellular esterase activity

Cited by 52 publications

References 30 publications

TAA1-Regulated Local Auxin Biosynthesis in the Root-Apex Transition Zone Mediates the Aluminum-Induced Inhibition of Root Growth inArabidopsis

TAA1-Regulated Local Auxin Biosynthesis in the Root-Apex Transition Zone Mediates the Aluminum-Induced Inhibition of Root Growth inArabidopsis

Growth of terrestrial cyanobacterium, Nostoc sp., on Martian Regolith Simulant and its vacuum tolerance

Cyanobacteria for Space Agriculture on Mars

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