A new methodology for electrostatic immobilization of a non-labeled single strand DNA onto a self-assembled diazonium modified gold electrode and detection of its hybridization by differential pulse voltammetry

Mashhadizadeh, Mohammad Hossein; Talemi, Rasoul Pourtaghavi

doi:10.1016/j.talanta.2012.10.069

Cited by 18 publications

(12 citation statements)

References 17 publications

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“…EIS can provide valuable information on the change in impedance behavior of the surface. 46,47 Thus, EIS technique was employed to investigate the interface properties of FTO, FTO/GNPs, and FTO/GNPs/Ap surfaces. The Nyquist plots are shown in Figure 3A.…”

Section: Characterization Of Modified Surfacesmentioning

confidence: 99%

Gold nanoparticles deposited on fluorine-doped tin oxide surface as an effective platform for fabricating a highly sensitive and specific digoxin aptasensor

2015

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In this paper we used a modified fluorine-doped tin oxide (FTO) as the substrate of a novel aptasensor for electrochemical determination of digoxin. For this purpose, a selective thiolated digoxin aptamer was immobilized onto the gold nanoparticles deposited FTO (GNPs/FTO) surface. Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and atomic force microscopy (AFM) were used for the characterization of modified surfaces. The aptasensor response was based on decrease in the methylene blue (MB) current, as an electrochemical probe, after incubating with digoxin. Digoxin can be determined in linear concentration range from 0.02 to 0.2 µg/L by differential pulse voltammetry (DPV). The detection limit of the proposed aptasensor was 0.01 µg/L. Also, the fabricated aptasensor was applied to determine digoxin in urine and blood plasma samples with satisfactory results. Furthermore, high sensitivity and specificity, low detection limit and fast response, can make it possible to determine trace amounts of digoxin for routine clinical analysis.

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Section: Characterization Of Modified Surfacesmentioning

confidence: 99%

Gold nanoparticles deposited on fluorine-doped tin oxide surface as an effective platform for fabricating a highly sensitive and specific digoxin aptasensor

2015

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“…However, the value was less than the theoretical value of 1.0 (adsorption-controlled process), illustrating that the oxidation of PMT at TiC/C also was an adsorption-controlled process [22,23]. The above results suggested that the oxidation of PMT at TiC/C was dominated by diffusion process accompanied by adsorption process [24].…”

Section: Resultsmentioning

confidence: 99%

A Sensitive Pyrimethanil Sensor Based on Electrospun TiC/C Film

Sui

Liu

et al. 2019

Sensors

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Titanium carbide (TiC) is a very significant transition metal carbide that displays excellent stability and electrical conductivity. The electrocatalytic activity of TiC is similar to noble metals but is much less expensive. Herein, carbon nanofibers (CNFs)-supported TiC nanoparticles (NPs) film (TiC/C) is prepared by electrospinning and carbothermal processes. Well-dispersed TiC NPs are embedded tightly into the CNFs frameworks. The electrochemical oxidation of pyrimethanil (PMT) at the TiC/C-modified electrode displays enhanced redox properties, and the electrode surface is controlled simultaneously both by diffusion and adsorption processes. When TiC/C is applied for PMT determination, the as-fabricated sensor shows good sensing performance, displaying a wide linear range (0.1–600 μM, R2 = 0.998), low detection limit (33 nM, S/N = 3), and good reproducibility with satisfied anti-interference ability. In addition, TiC/C shows long-term stability and good application in natural samples. The facile synthetic method with good sensing performance makes TiC/C promising as novel electrode materials to fabricate efficient sensors.

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“…63 The DNA capture probes having negatively charged phosphate backbones are immobilised to the positively charged diazonium salt via ionic bonding. 63 The DNA capture probes having negatively charged phosphate backbones are immobilised to the positively charged diazonium salt via ionic bonding.…”

Section: The Selection Of Capture Probesmentioning

confidence: 99%

Electrochemical nucleic acid biosensors: from fabrication to application

et al. 2016

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Electrochemical biosensors have been around since the 1960s. They are among the most researched and applied biosensors. Their high portability and biocompatibility have seen them being used in the construction of in vivo biosensors. In the past three decades, their applications have been broadened from the detection of small molecules like glucose to nucleic acids and proteins. In particular, leveraging the specific base-pairing mechanism of nucleic acids, impressive progress has been achieved in the field of electrochemical nucleic acid biosensors. Moreover, various signal amplification strategies in conjunction with microfabrication technology have been extensively studied and have resulted in significant improvements in the sensitivity and multiplexing capability of electrochemical nucleic acid biosensors. In this article, the progress in the research and applications of electrochemical nucleic acid biosensors is reviewed with an emphasis on the principal components of electrochemical nucleic acid biosensors, their construction, sensing mechanism and technical applications with some discussion on challenges and perspectives.

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A new methodology for electrostatic immobilization of a non-labeled single strand DNA onto a self-assembled diazonium modified gold electrode and detection of its hybridization by differential pulse voltammetry

Cited by 18 publications

References 17 publications

Gold nanoparticles deposited on fluorine-doped tin oxide surface as an effective platform for fabricating a highly sensitive and specific digoxin aptasensor

Gold nanoparticles deposited on fluorine-doped tin oxide surface as an effective platform for fabricating a highly sensitive and specific digoxin aptasensor

A Sensitive Pyrimethanil Sensor Based on Electrospun TiC/C Film

Electrochemical nucleic acid biosensors: from fabrication to application

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