A New Method Without Organic Solvent to Targeted Nanodrug for Enhanced Anticancer Efficacy

Abstract

Since the hydrophobic group is always essential to the synthesis of the drug-loaded nanoparticles, a majority of the methods rely heavily on organic solvent, which may not be completely removed and might be a potential threat to the patients. In this study, we completely “green” synthesized 10-hydroxycamptothecine (HCPT) loaded, folate (FA)-modified nanoneedles (HFNDs) for highly efficient cancer therapy with high drug loading, targeting property, and imaging capability. It should be noted that no organic solvent was used in the preparation process. In vitro cell uptake study and the in vivo distribution study showed that the HFNDs, with FA on the surface, revealed an obviously targeting property and entered the HeLa cells easier than the chitosan-HCPT nanoneedles without FA modified (NDs). The cytotoxicity tests illustrated that the HFNDs possessed better killing ability to HeLa cells than the individual drug or the NDs in the same dose, indicating its good anticancer effect. The in vivo anticancer experiment further revealed the pronounced anticancer effects and the lower side effects of the HFNDs. This new method without organic solvent will lead to a promising sustained drug delivery system for cancer diagnosis and treatment.

Advances in Antitumor Nano-Drug Delivery Systems of 10-Hydroxycamptothecin

“… 200 FA-modified chitosan nanoneedles ( Figure 5 ) and micelles exhibit significantly enhanced cellular uptake, higher cytotoxicity against folate-receptor-positive tumor cells. 201 , 202 Loaded with HCPT and MTX-dual drug chitosan NPs that exhibit sustained and prolonged drug release properties, as well as effective cellular internalization and enhanced cytotoxicity. 203 Studies have shown that nitric oxide (NO) is an endogenous gas transmitter can overcome multidrug resistance (MDR) by making nitrification of certain tyrosine residue of P-glycoprotein (Pgp) to inhibit the pumping function.…”

“…In vitro and In vivo [183] Electrospun fibers-based NPs Chemotherapy In vivo [185,192] Chemotherapy In vitro and In vivo [186][187][188]190] Polysaccharide-based (Chitosan) NPs Chemotherapy In vitro [197,199] Chemotherapy In vitro and In vivo [198,[201][202][203] Chemotherapycombined with Gas Therapy In vitro and In vivo [ In vivo [284] Chemotherapy In vitro and In vivo [281,282,285,286] Chemotherapy combined with PDT In vitro and In vivo [278,279] https://doi.org/10.2147/IJN.S377149…”

The Confluence of Innovation in Therapeutics and Regulation: Recent CMC Considerations

“…In addition, the manufacturing of nanoparticles typically requires the use of organic solvents, which can be difficult to completely remove from the final formulation. 47…”

Dual Drug Loaded, pH-Sensitive Metal-Organic Particles for Synergistic Cancer Therapy

“…In these NDDSs, various functional molecules were integrated to guide the particles to accumulate at the tumor site ( Wang et al, 2017 ). Various targeting molecules, such as folate ( Wu et al, 2017b ; Hamdy Elshazly et al, 2019 ), methotrexate ( Li et al, 2015 ), and hyaluronic acid ( Misra, 2010 ; Choi et al, 2019 ), were selected to modify the NDDSs. They could bind with the specific receptors on the tumor cells to achieve targeted delivery.…”