A New Method to Bind Allergens for the Measurement of Specific IgE Antibodies

Sander, I.; Kespohl, S.; Merget, R.; Goldscheid, N.; Degens, Paul O.; Brüning, Thomas; Raulf‐Heimsoth, Monika

doi:10.1159/000082583

Cited by 64 publications

(50 citation statements)

References 42 publications

(24 reference statements)

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“…Biotiniled Cannabis extracts were coupled to Streptavidin-ImmunoCAP solid phase (Streptavidin-ImmunoCAP, Phadia AB. Uppsala, Sweden), according to Sander et al (2005). 16 Specific IgE to Cannabis was determined by the ImmunoCAP System using the above mentioned solid-phases.…”

Section: In Vitro Tests Specific Igementioning

confidence: 99%

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Allergic hypersensitivity to cannabis in patients with allergy and illicit drug users

Armentia

Castrodeza²,

Ruiz-Muñoz³

et al. 2011

Allergologia et Immunopathologia

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Background: Cannabis is the illicit drug most widely used by young people in high-income countries. Allergy symptoms have only occasionally been reported as one of the adverse health effects of cannabis use. Objectives: To study IgE-mediated response to cannabis in drug users, atopic patients, and healthy controls. Methods: Asthmatic patients sensitised to pollen, and all patients sensitised to tobacco, tomato and latex, considered as cross-reacting allergens, were selected from a data base of 21,582 patients. Drug users attending a drug-rehabilitation clinic were also included. Controls were 200 non-atopic blood donors. Specific IgE determination, prick tests and specific challenge with cannabis extracts were performed in patients and controls. Results: Overall, 340 patients, mean age 26.9 ± 10.7 years, were included. Males (61.4%) were the most sensitised to cannabis (p < 0.001). All cannabis-sensitised patients were alcohol users. Eighteen (72%) of the patients allergic to tomato were sensitised to cannabis, but a positive specific challenge to cannabis was highest in patients sensitised to tobacco (13/21, 61.9%), (p < 0.001). Pollen allergy was not a risk factor for cannabis sensitisation. Prick tests and IgE for cannabis had a good sensitivity (92 and 88.1%, respectively) and specificity (87.1 and 96%) for cannabis sensitisation. Conclusions: Cannabis may be an important allergen in young people. Patients previously sensitised to tobacco or tomato are at risk. Cannabis prick tests and IgE were useful in detecting sensitisation.

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Section: In Vitro Tests Specific Igementioning

confidence: 99%

“…18 The effect of cannabis on pulmonary function is not clear. 16 Habitual users have more symptoms of bronchitis and respiratory infections. Cannabis does not seem to increase the risk of emphysema 19,20 and obstructive patterns improved in some of our patients after smoking cannabis.…”

Section: Might Cannabis Be An Allergen?mentioning

confidence: 99%

Allergic hypersensitivity to cannabis in patients with allergy and illicit drug users

Armentia

Castrodeza²,

Ruiz-Muñoz³

et al. 2011

Allergologia et Immunopathologia

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“…The three native latex allergens were prepared at the Rubber Research Institute in Malaysia [18,19], purified and coupled to streptavidin Immuno-CAP TM as described [20]. All recombinant latex allergens were produced in E. coli in fusion with maltose-binding protein (MBP) [6][7][8][21][22][23].…”

Section: Native and Recombinant Latex Allergensmentioning

confidence: 99%

Quantitative analysis of immunoglobulin E reactivity profiles in patients allergic or sensitized to natural rubber latex (Hevea brasiliensis)

Raulf‐Heimsoth

Rihs

Rozynek

et al. 2007

Clin Experimental Allergy

108

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SummaryBackground Characterized native and recombinant Hevea brasiliensis (rHev b) natural rubber latex (NRL) allergens are available to assess patient allergen sensitization profiles.Objective Quantification of individual IgE responses to the spectrum of documented NRL allergens and evaluation of cross-reactive carbohydrate determinants (CCDs) for more definitive diagnosis. Methods Sera of 104 healthcare workers (HCW; 51 German, 21 Portuguese, 32 American), 31 spina bifida patients (SB; 11 German, 20 Portuguese) and 10 Portuguese with multiple surgeries (MS) were analysed for allergen-specific IgE antibody (sIgE) to NRL, single Hev b allergens and CCDs with ImmunoCAP TM technology. Results In all patient groups rHev b 5-sIgE concentrations were the most pronounced. Hev b 2, 5, 6.01 and 13 were identified as the major allergens in HCW and combined with Hev b 1 and Hev b 3 in SB. In MS Hev b 1 displayed an intermediate relevance. Different sIgE antibody levels to native Hevea brasiliensis (nHev b) 2 and rHev b 6.01 allowed discrimination of SB with clinical relevant latex allergy vs. those with latex sensitization. Sensitization profiles of German, Portuguese and American patients were equivalent. rHev b 5, 6.01 and nHev b 13 combined detected 100% of the latex-allergic HCW and 80.1% of the SB. Only 8.3% of the sera showed sIgE response to CCDs. Conclusions Hev b 1, 2, 5, 6.01 and 13 were identified as the major Hev b allergens and they should be present in standardized latex extracts and in vitro allergosorbents. CCDs are only of minor relevance in patients with clinical relevant latex allergy. Component-resolved diagnostic analyses for latex allergy set the stage for an allergen-directed immunotherapy strategy.

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“…Even though the exposure test was carried out in the workplace instead of a IgG to Aspergillus fumigatus (gm3) 26 mgA?L -1 # : biotinylated antigen/allergen bound to Streptavidin (ImmunoCAP; Phadia, Uppsala, Sweden) as described previously [4].…”

Section: Discussionmentioning

confidence: 99%

Hypersensitivity pneumonitis caused by occupational exposure to phytase

Heemst¹,

Sander²,

Rooyackers³

et al. 2009

European Respiratory Journal

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A 43-yr-old male presented with a 6-month history of episodes of coughing, shortness of breath and fever. He suffered from dyspnoea on minor exertion. The patient worked in a cattle feed factory and noticed that he had more complaints after his working hours. His symptoms could be ascribed to hypersensitivity pneumonitis due to contact with phytase, an enzyme added to cattle feed to strengthen bone and diminish phosphorus excretion.The diagnosis was supported by bibasal lung crackles on physical examination, restrictive ventilatory defect (with decreased diffusion capacity for carbon monoxide), typical radiographical findings, lymphocytosis in bronchoalveolar lavage fluid and a positive exposure test performed at the workplace. Blood examination showed high immunoglobulin G levels to phytase.After treatment and cessation of phytase contact the patient became symptom free and lung function normalised. Phytase should be considered as a cause of occupational hypersensitivity pneumonitis in the animal feed industry.KEYWORDS: Extrinsic allergic alveolitis, hypersensitivity pneumonitis, phytase CASE REPORT A 43-yr-old male without prior illness was referred to the outpatient clinic of our department with symptoms of cough and shortness of breath on minor exertion, fever and malaise (especially fatigue). He had lost 8 kg of weight since his complaints had started 6 months earlier and had been a non-smoker for 20 yrs. There was a relationship between his complaints and his working hours. He had worked in a factory that produces cattle feed for .20 yrs where his role was to take samples of the cattle feed at different departments on different floors for quality control. He noticed that after having taken samples on one particular floor, 6-8 h later, his complaints started. The next morning the complaints had almost disappeared. He had no symptoms during the holidays. None of his colleagues (n521) had similar symptoms.At physical examination pulse and blood pressure were normal. At auscultation of the lungs normal breath sounds were heard but with bibasal crackles. Erythrocyte sedimentation rate was 33 mm?h -1 and C-reactive protein was 11 mg?L ). Blood leukocytes, total immunoglobulin (Ig)E, antinuclear antibodies and specific IgE for common aeroallergens were all within reference values.

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A New Method to Bind Allergens for the Measurement of Specific IgE Antibodies

Cited by 64 publications

References 42 publications

Allergic hypersensitivity to cannabis in patients with allergy and illicit drug users

Allergic hypersensitivity to cannabis in patients with allergy and illicit drug users

Quantitative analysis of immunoglobulin E reactivity profiles in patients allergic or sensitized to natural rubber latex (Hevea brasiliensis)

Hypersensitivity pneumonitis caused by occupational exposure to phytase

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