A new method to amplify colorimetric signals of paper-based nanobiosensors for simple and sensitive pancreatic cancer biomarker detection

Prasad, K. Sudhakara; Abugalyon, Yousef; Li, Chunqiang; Xu, Feng; Li, Xiujun

doi:10.1039/d0an00704h

Cited by 34 publications

(20 citation statements)

References 48 publications

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“…A systematic understanding of AuNP aggregation is not only fundamentally interesting but also practically important for sensing. In fact, beyond colorimetric sensing, the aggregation of AuNPs has also been used for many other sensing mechanisms, such as the photothermal effect, catalysis, , and fluorescence quenching . In this work, we systematically explored both the cations and anions of various salts to understand their effect on AuNP stability in the absence and presence of a DNA oligonucleotide.…”

Section: Introductionmentioning

confidence: 99%

Dissecting the Effect of Salt for More Sensitive Label-Free Colorimetric Detection of DNA Using Gold Nanoparticles

Huang

Wang

et al. 2020

Anal. Chem.

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Taking advantage of the protection effect of singlestranded DNA oligonucleotides, gold nanoparticles (AuNPs) remain dispersed and retain a red color with the addition of a low concentration of salt, while AuNPs would aggregate in the presence of double-stranded DNA. This difference has been used to design label-free colorimetric sensors for DNA detection. NaCl is the most commonly used salt to induce the aggregation of AuNPs. In this work, we aimed to test if other salts can provide even better sensor performance and to understand the effects of the cations and anions in salts. We first studied the effect of anions, including halides (NaF, NaCl, NaBr, and NaI), and other common salts (NaNO 3 , NaClO 4 , Na 2 SO 4 , Na 2 S 2 O 3 , sodium phosphate, and sodium citrate). Among them, weakly adsorbing ones such as F − , citrate, and phosphate appeared to yield better sensitivity than Cl − . Anions can directly adsorb on the AuNPs and affect DNA adsorption. We then tested cations, and only group 1A metals (LiCl, NaCl, KCl, RbCl, and CsCl) can signal DNA adsorption, while divalent metals (MgCl 2 , CaCl 2 , MnCl 2, and NiCl 2 ) barely showed the effect of DNA. CsCl only works for strongly adsorbing DNA, such as A 15 , but not weakly adsorbing T 15 . Overall, NaF is a better salt than NaCl by having a 2.3-fold higher sensitivity, which was confirmed in a DNA sensing assay. This work has identified a better salt yielding higher sensitivity, and sensing work relying on the change of the aggregation state of AuNPs can benefit from this study.

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Section: Introductionmentioning

confidence: 99%

Dissecting the Effect of Salt for More Sensitive Label-Free Colorimetric Detection of DNA Using Gold Nanoparticles

Huang

Wang

et al. 2020

Anal. Chem.

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“…Since the existing methods for early diagnosis of pancreatic cancer are unreliable, the emergence of such technology is of practical significance for promoting the progress of early screening of this type of cancer. Another paper-based method for sensitive detection of PEAK1 was created [ 165 ] that directly observed the results by colorimetry. This technique, utilizing the chemical characteristics of AuNPs that can catalyze the degradation of color dyes, can complete the detection process without specific instruments.…”

Section: Applications Of μPads In Ivdmentioning

confidence: 99%

Research Progress and Future Trends of Microfluidic Paper-Based Analytical Devices in In-Vitro Diagnosis

et al. 2022

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In vitro diagnosis (IVD) has become a hot topic in laboratory research and achievement transformation. However, due to the high cost, and time-consuming and complex operation of traditional technologies, some new technologies are being introduced into IVD, to solve the existing problems. As a result, IVD has begun to develop toward point-of-care testing (POCT), a subdivision field of IVD. The pandemic has made governments and health institutions realize the urgency of accelerating the development of POCT. Microfluidic paper-based analytical devices (μPADs), a low-cost, high-efficiency, and easy-to-operate detection platform, have played a significant role in advancing the development of IVD. μPADs are composed of paper as the core material, certain unique substances as reagents for processing the paper, and sensing devices, as auxiliary equipment. The published reviews on the same topic lack a comprehensive and systematic introduction to μPAD classification and research progress in IVD segmentation. In this paper, we first briefly introduce the origin of μPADs and their role in promoting IVD, in the introduction section. Then, processing and detection methods for μPADs are summarized, and the innovative achievements of μPADs in IVD are reviewed. Finally, we discuss and prospect the upgrade and improvement directions of μPADs, in terms of portability, sensitivity, and automation, to help researchers clarify the progress and overcome the difficulties in subsequent μPAD research.

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“…In our previous works, we have developed three generations of highly fluorescent semiconducting polymer nanoparticle (Pdot)-based ICTS platforms for quantitatively determining several cancer markers with the sensitivity of 1-2 orders of magnitude lower than that of the traditional colorimetric ICTS. − The superior sensitivity of the Pdot-based ICTS can be ascribed to the unique properties of Pdots: − (i) phenomenal fluorescence brightness (usually 3 orders of magnitude brighter than small dyes), providing good signal-to-background ratios; (ii) excellent colloidal stability in physiological environments; and (iii) easy surface engineering for bioconjugation with specific targets. In this work, we aimed to design a colorimetric and fluorescent dual-mode ICTS platform based on Pdots for simultaneous detection of CEA and CYFRA21-1 in human whole blood.…”

Section: Introductionmentioning

confidence: 99%

Bimodal Multiplexed Detection of Tumor Markers in Non-Small Cell Lung Cancer with Polymer Dot-Based Immunoassay

et al. 2021

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Semiconducting polymer nanoparticles (Pdots) have been demonstrated to be a promising class of probes for use in fluorometric immunochromatographic test strips (ICTS). The advantages of Pdots in ICTSs include ultrahigh brightness, minimal nonspecific adsorption, and multicolor availability, which together contribute to the high sensitivity, good specificity, and multiplexing ability. These unique properties can therefore circumvent several significant challenges of commercial ICTSs, including insufficient specificity/sensitivity and difficulty in quantitative and multiplexed detection. Here, we developed a colorimetric and fluorescent bimodal readout ICTS based on gold-Pdot nanohybrids for the determination of carcinoembryonic antigen (CEA) and cytokeratin 19 fragment (CYFRA 21-1) expressed abnormally in human blood of non-small-cell lung cancer (NSCLS). The vivid color from Au nanomaterials can be used for rapid qualitative screening (colorimetry) in 15 min, while the bright fluorescence of Pdots is ideal for the advanced quantitative measurements of CEA and CYFRA21-1 concentrations in whole blood samples. This bimodal ICTS platform possesses phenomenal detection sensitivity of 0.07 and 0.12 ng/mL for CYFRA21-1 and CEA, respectively. The accuracy and reliability of this ICTS platform were further evaluated with clinical serum samples from NSCLS patients at different stages, showing good consistency with the results from electrochemiluminescence immunoassay.

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A new method to amplify colorimetric signals of paper-based nanobiosensors for simple and sensitive pancreatic cancer biomarker detection

Abstract: Catalytic properties of gold nanoparticles in colour dye degradation are utilized to amplify colorimetric detection signals of a low-cost paper-based immunosensor for instrument-free detection of pancreatic cancer biomarker PEAK1.

Cited by 34 publications

References 48 publications

Dissecting the Effect of Salt for More Sensitive Label-Free Colorimetric Detection of DNA Using Gold Nanoparticles

Dissecting the Effect of Salt for More Sensitive Label-Free Colorimetric Detection of DNA Using Gold Nanoparticles

Research Progress and Future Trends of Microfluidic Paper-Based Analytical Devices in In-Vitro Diagnosis

Bimodal Multiplexed Detection of Tumor Markers in Non-Small Cell Lung Cancer with Polymer Dot-Based Immunoassay

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