A New Method of Using Polyethylene Glycol (PEG) Precipitation of Macroprolactin to Detect Genuine Hyperprolactinemia

Chen, Yongjian; Wang, Huan; Yang, Wei; Jin, Weidong; Yu, Wenge; Wang, Wei; Zhang, Kailin; Song, Guangzhong

doi:10.1002/jcla.21999

Cited by 11 publications

(8 citation statements)

References 25 publications

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“…Des Weiteren muss die Makroprolaktinämie von der "echten" Hyperprolaktinämie abgegrenzt werden. Prolaktin kann im Serum in biologisch aktiver monomerischer Form oder in biologisch gering aktiver Aggregatform gebunden an seinen Antikörper, als sogenanntes Makroprolaktinom, zirkulieren, was durch herkömmliche Assays nicht sicher unterschieden wird [21]. Immer, wenn eine Hyperprolaktinämie ohne jegliche Symptomatik vorliegt, sollte diese Makroprolaktinämie ausgeschlossen werden.…”

Section: Pathophysiologie Und Technische Aspekteunclassified

Hyperprolaktinämie aus gynäkologischer Sicht

Hancke¹,

Weiß²

2020

J. Klin. Endokrinol. Stoffw.

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Zusammenfassung Eine Hyperprolaktinämie kann idiopathisch bedingt sein oder physiologisch im Rahmen von Schwangerschaft, Stillzeit, Stress, Schlaf oder körperlicher Aktivität vorkommen. Andererseits können eine Hypophysen- oder Hypophysenstielpathologie, Wechselwirkungen durch Medikamente, eine Hypothyreose oder Niereninsuffizienz eine Hyperprolaktinämie verursachen. Die Symptome können ganz gering ausgeprägt sein oder sich sehr vielfältig im Sinne einer Oligo‑/Amenorrhö, einer Galaktorrhö oder eines unerfüllten Kinderwunsches darstellen. In dieser Übersichtsarbeit sollen die verschiedenen Aspekte der Hyperprolaktinämie aus gynäkologischer Sicht dargestellt werden.

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Section: Pathophysiologie Und Technische Aspekteunclassified

Hyperprolaktinämie aus gynäkologischer Sicht

Hancke¹,

Weiß²

2020

J. Klin. Endokrinol. Stoffw.

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“…Macroprolactin has a lower biological effect, so fewer investigation and management changes are needed as it is unlikely to cause symptoms. Hyperprolactinaemia caused by raised macroprolactin can be detected by polyethylene glycol (PEG) precipitation, which is the most common method for assessing for macroprolactin levels in clinical laboratories (Chen 2016). Some laboratories automatically test macroprolactin levels following a high prolactin result.…”

Section: Checking Prolactinmentioning

confidence: 99%

Hyperprolactinaemia in the context of psychiatry

et al. 2020

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SUMMARY Advocating for good physical healthcare for their patients is of the utmost importance to psychiatrists. This narrative review focuses on one part of this large goal, the topic of hyperprolactinaemia from the perspective of mental healthcare. For psychiatrists this often includes managing raised prolactin levels in the context of medication. However, they must consider the wider differentials of a raised prolactin level and the possible impact of treatments. For that reason, in this review we start with an overview of prolactin physiology before considering hyperprolactinaemia both in the context of antipsychotic therapy and its wider differentials, including prolactinoma. Investigation and management are considered and key practice points developed.

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“…Large proteins or complexes are precipitated by PEG by steric exclusion only if they are surrounded by linear PEG chains [25,26]. Although numerous methods are available for recovery of ICs by PEG precipitation [5,7,[23][24][25][26][27][28][29][30][31][32], very few studies have identified the antigen within ICs.…”

Section: Introductionmentioning

confidence: 99%

A proteomics-based method for identifying antigens within immune complexes

Menikou

McArdle

et al. 2020

PLoS ONE

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A novel approach to recover and identify immune complexes (ICs) was developed using size exclusion chromatography (SEC) and affinity chromatography on immunoglobulin binding columns (HiTrap Protein G). The purification process was monitored by 1D SDS-PAGE, protein staining, Western blotting and, finally, liquid chromatography tandem mass spectrometry (LC MS/MS) was used to identify the recovered antigens. This approach was applied to serum with artificially created immune complexes (ICs) comprising vaccine antigen (influenza) and antibody, which led to recovery and identification of influenza peptides within the recovered ICs. This approach was compared with the established method for IC detection and recovery, polyethylene glycol (PEG) precipitation, followed by LC MS/MS. Both approaches successfully enabled capture, recovery and characterization of immunoglobulins and influenza antigen(s) in complex with the immunoglobulins. However, PEG precipitation has the advantage of simplicity and is more suited for large scale studies.

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A New Method of Using Polyethylene Glycol (PEG) Precipitation of Macroprolactin to Detect Genuine Hyperprolactinemia

Abstract: Precipitation using 5-fold diluted serum with 25% PEG6000 can effectively reduce the macroPRL concentration, increasing the PRL recovery rate and detection rate of genuine HP after precipitation, which is an effective and convenient method for the detection of genuine HP.

Cited by 11 publications

References 25 publications

Hyperprolaktinämie aus gynäkologischer Sicht

Hyperprolaktinämie aus gynäkologischer Sicht

Hyperprolactinaemia in the context of psychiatry

A proteomics-based method for identifying antigens within immune complexes

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