A new method of measuring albumin permeability in isolated glomeruli

Godfrin, Yann; Dantal, Jacques; Bouhours, Jean-François; Heslan, Jean‐Marie; Soulillou, Jean‐Paul

doi:10.1038/ki.1996.448

Cited by 30 publications

(20 citation statements)

References 21 publications

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“…The structure and origin of the factor are still unknown. The presence of this permeability factor has been used to evaluate the risk of recurrence and to guide therapy but conflicting results have been published (21)(22)(23). Nevertheless, the beneficial effect of PE observed in some patients might be related to the removal of this factor.…”

Section: Discussionmentioning

confidence: 95%

Intravenous cyclosporine therapy in recurrent nephrotic syndrome after renal transplantation in children

Salomon¹,

Gagnadoux²,

Niaudet³

2003

Transplantation

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Section: Discussionmentioning

confidence: 95%

Intravenous cyclosporine therapy in recurrent nephrotic syndrome after renal transplantation in children

Salomon¹,

Gagnadoux²,

Niaudet³

2003

Transplantation

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“…They used isolated glomeruli incubated in a hypotonic medium to detect the presence of this serum factor. If normal serum was added to the medium, fluid moved into the capillary lumen and the glomerular volume increased [15,16]. If serum of some of the FSGS patients was added to the hypotonic medium, the glomerular volume increased but not as much as in controls [15].…”

Section: Circulating Plasma Factors Thought To Cause Proteinuria In Imentioning

confidence: 99%

Circulating mediators of proteinuria in idiopathic minimal lesion nephrotic syndrome

Garin

2000

Pediatric Nephrology

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The pathogenesis of proteinuria in idiopathic minimal lesion nephrotic syndrome (IMLNS) remains to be elucidated. The most-accepted hypothesis is that the increased glomerular permeability to plasma proteins results from the effect of circulating factors on glomerular capillaries. This report critically reviews the current studies that have attempted to isolate and characterize this putative factor(s). Products released from hepatocyte or peripheral blood mononuclear cells or isolated by chromatography from serum or plasma have been tested in rats for their role in inducing proteinuria. These factors have been infused into the isolated kidney preparation or into the intact animal as a single venous injection, or continuously by pump for a period of 4 h to 7 days. Several of these isolated factors have been shown to induce proteinuria in rats. However, their exclusive pathogenetic role is questionable since none is always present in all IMLNS patients during relapse. Therefore, the increase in proteinuria in these patients may result from a single or a variety of factors as yet to be identified.

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“…47 Glomerular pellets were lysed (in 2% TritonX-100, 250 mM NaCl, 20 mM Tris, 2 mM EDTA, 2 mM EGTA, 4 mM Na 3 VO 4 , 10 mM Na 4 P 2 O 7 , 50 mM NaF, 40 M leustatin, 0.4 mM PMSF, and 40 M pepstatin), and their concentrations were measured using the BCA protein assay (Bio-Rad, Hercules, CA). A total of 20 g of each lysate was used for Western blot experiments according the Bio-Rad instructions (SDS-PAGE 10%; rabbit anti-IB␣ or rabbit anti-phosphorylated IB␣ [1:1000; Cell Signaling Technology, Danvers, MA] or mouse anti-active NF-KB p65 [10 g/ml; Chemicon Europe, Hampshire, UK] antibodies; peroxidase-conjugated goat anti-rabbit IgG and donkey anti-mouse IgG [1:1000 and 1:3000, respectively; Jackson ImmunoResearch]).…”

Section: Immunoblottingmentioning

confidence: 99%

Induction of T Regulatory Cells Attenuates Idiopathic Nephrotic Syndrome

Berre¹,

Bruneau²,

Naulet³

et al. 2009

Journal of the American Society of Nephrology

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102

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Buffalo/Mna rats spontaneously develop FSGS and nephrotic syndrome as a result of an immune disorder. Similar to some humans with FSGS, the disease recurs after renal transplantation, suggesting the involvement of a circulating factor. Here, we tested the effect of several immunosuppressive treatments on these rats. Although corticosteroids, cyclosporin A, and anti-T cell receptor treatment reduced proteinuria, only the deoxyspergualin derivative LF15-0195 led to a rapid and complete normalization of proteinuria. Furthermore, this compound led to the regression of renal lesions during both the initial disease and posttransplantation recurrence. The frequency of splenic and peripheral CD4 ϩ CD25 ϩ FoxP3 ϩ T lymphocytes significantly increased with remission. Moreover, the transfer of purified LF15-0195-induced CD4 ϩ CD25 ϩ T cells to irradiated Buff/Mna rats significantly reduced their proteinuria compared with the transfer of untreated control cells, suggesting that LF15-0195 induces regulatory T cells that are able to induce regression of rat nephropathy. These data suggest that idiopathic nephrotic syndrome/FSGS disease can be regulated by cellular transfer, but how this regulation leads to the reorganization of the podocyte cytoskeleton remains to be determined.

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A new method of measuring albumin permeability in isolated glomeruli

Cited by 30 publications

References 21 publications

Intravenous cyclosporine therapy in recurrent nephrotic syndrome after renal transplantation in children

Intravenous cyclosporine therapy in recurrent nephrotic syndrome after renal transplantation in children

Circulating mediators of proteinuria in idiopathic minimal lesion nephrotic syndrome

Induction of T Regulatory Cells Attenuates Idiopathic Nephrotic Syndrome

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