A New Method of Harvesting Descemet Membrane-Endothelium Complex Approaching From the Suprachoroidal Space for Descemet Membrane Endothelial Keratoplasty: An Experimental Animal Study

Chang, Suyon; Jun, Jong Hwa; Hong, Kwon Ho

doi:10.1097/ico.0b013e3181c6b637

Cornea

2010

DOI: 10.1097/ico.0b013e3181c6b637

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A New Method of Harvesting Descemet Membrane-Endothelium Complex Approaching From the Suprachoroidal Space for Descemet Membrane Endothelial Keratoplasty: An Experimental Animal Study

Suyon Chang

Jong Hwa Jun²,

Kwon Ho Hong³

Abstract: The new method of en bloc harvesting Descemet membrane-endothelium complex approaching from the suprachoroidal space could provide minimal damage to the endothelium during harvesting, and it can be carried and passed through an injector.

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2014

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Preparation, preservation, and morphological evaluation of the donor graft for descemet membrane endothelial keratoplasty: an experimental study

Sun,

Peng,

Hong

2014

Chinese Medical Journal

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Background Though there have been various methods for harvesting and preserving descemet membrane (DM) and intact endothelium, there is no literature about the morphological evaluation of endothelium after graft preparation for descemet membrane endothelial keratoplasty (DMEK). The aim of this study was to establish and improve a simple method for preparing, preserving, and morphologically evaluating the donor graft for DMEK. Methods To obtain a donor graft, an air bubble was formed by injecting a 29 G needle with 1 ml sterile air into a small edge created outside the Schwalbe line. Another needle was inserted into the bubble through the stroma to aspirate the air or replace half the air with organ culture medium. Trypan blue was used to mark the location for small incision to improve the success rate. Frozen sections were stained with hematoxylin and eosin (HE). Based on the air bubble, DM grafts were divided into four groups: group A (normal control), graft without any operative technique; group B, graft with zero-pressure air bubble; group C, graft with full-pressure air bubble; group D, graft with half-pressure air bubble. The four groups of grafts were preserved for 24 hours to observe the effect of bubbles on cells. The gross and ultrastructure morphologies were evaluated using alizarin red and scanning electron microscopy (SEM), respectively. Results Donor grafts were harvested via the air bubble technique, facilitated by prior trypan blue staining. HE-stained sections revealed a pure graft without stroma. There were no significant changes under light microscope. In group A, SEM revealed a confluent layer of polygonal endothelium with distributed microvilli exhibiting characteristics of interdigitating junctions. In group B, intercellular borders became thinner. In group C, interdigitations were almost flat and microvilli were observed less frequently. In group D, other than less microvilli, there were minimal changes. Conclusions The donor graft preparation method appears to be effective and convenient. Properly decreasing the air pressure could protect and preserve the endothelium.

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Preparation, preservation, and morphological evaluation of the donor graft for descemet membrane endothelial keratoplasty: an experimental study

Sun,

Peng,

Hong

2014

Chinese Medical Journal

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A New Method of Harvesting Descemet Membrane-Endothelium Complex Approaching From the Suprachoroidal Space for Descemet Membrane Endothelial Keratoplasty: An Experimental Animal Study

Abstract: The new method of en bloc harvesting Descemet membrane-endothelium complex approaching from the suprachoroidal space could provide minimal damage to the endothelium during harvesting, and it can be carried and passed through an injector.

Cited by 1 publication

References 26 publications

Preparation, preservation, and morphological evaluation of the donor graft for descemet membrane endothelial keratoplasty: an experimental study

Preparation, preservation, and morphological evaluation of the donor graft for descemet membrane endothelial keratoplasty: an experimental study

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