A new method of estimating the proportions of different proteins in a mixture using amino acid profiles: application to undigested proteins in the preruminant calf

Duvaux, C; Guilloteau, Paul; Toullec, R.; Sissons, JW

doi:10.1051/animres:19900102

Cited by 28 publications

(25 citation statements)

References 7 publications

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“…The proportions of dietary, endogenous and bacterial proteins which could be the main constituents of digesta protein were assessed by the method developed by Duvaux et al (1990). This method uses a multiple regression analysis to establish the theoretical mixture which minimizes the X 2 distance with regard to the AA composition of digesta.…”

Section: Discussionmentioning

confidence: 99%

Digestibility and amino acid composition of digesta at the end of the ileum in preruminant calves fed soyabean protein

Caugant¹,

Toullec²,

Formal³

et al. 1993

Reprod. Nutr. Dev.

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Summary ― The ileal digestion of 3 milk substitutes in which skim milk powder was either the only protein source (control diet) or was partially replaced (50%) by a heated soyabean flour (SF diet) or an alcohol-treated soyabean protein concentrate (SC diet) was studied in 6 preruminant calves which were fitted with an ileo-caecal re-entrant cannula. The apparent digestibility of total nitrogen and amino acids was lower with the SF and SC diets than with the control diet (0.89, 0.89 and 0.94, respectively, for amino acid nitrogen). Assuming that true digestibility was complete with the control diet, the values were lower with the SF and SC diets, especially for cystine, threonine, valine, isoleucine, leucine and histidine. With the SF and SC diets, digesta contained more aspartic acid and glutamic acid but less threonine, sulfur amino acids, lysine, serine and alanine than with the control diet. The additional undigested fractions obtained with the SF and SC diets compared to the control diet were rich in aspartic and glutamic acids, and poor in arginine, suggesting that partially degraded dietary fractions different from the whole soyabean escaped digestion in the small intestine; these fractions probably originated mainly from glycinin. No significant differences were observed between the 2 soyabean diets.

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Section: Discussionmentioning

confidence: 99%

Digestibility and amino acid composition of digesta at the end of the ileum in preruminant calves fed soyabean protein

Caugant¹,

Toullec²,

Formal³

et al. 1993

Reprod. Nutr. Dev.

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“…First, the proportions of endogenous and dietary proteins in ileal digesta were estimated according to Duvaux et al (1990) who developed a mathematical model to calculate the proportions of different "reference" proteins in a mixture, from their AA profiles. This method combines a multiple regression analysis, without intercept, and the calculation of the deviation between the actual and expected AA composition of a mixture of proteins by means of a χ 2 value.…”

Section: Estimates Of Endogenous Gut Aa Lossesmentioning

confidence: 99%

“…As specified by Duvaux et al (1990) and in order to assign the same weight to each AA, the square-root of the AA proportions in digesta protein were considered as dependent variables; the ratios of AA proportions in "reference" proteins to this square-root were considered as independent variables. The χ 2 value, representing the extent of the agreement between the actual and the expected AA composition of digesta protein, was calculated as follows:…”

Section: Estimates Of Endogenous Gut Aa Lossesmentioning

confidence: 99%

“…In particular, differences in available N for bacterial growth may cause changes in the AA pattern in digesta (Bartelt et al 1997;Caine et al 1999) and, therefore, bias the estimates of endogenous losses. However, Schulze et al (1993) showed with skimmilk powder, soybean meal, soy isolate and fish meal based diets, that, using the AA pattern of the dietary protein and of the endogenous protein of pigs fed a protein-free diet as reference proteins, the regression method proposed by Duvaux et al (1990), provided estimates comparable to measured values obtained by 15 N dilution using an intravenous 15 N-L-leucine infusion.…”

Section: True Digestibility Values and Estimates Of Endogenous Aa Lossesmentioning

confidence: 99%

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Ileal amino acid digestibility and estimates of endogenous amino acid losses in pigs fed rapeseed meal, sunflower meal and soybean meal

Jondreville¹,

Broecke²,

Gatel³

et al. 2000

Can. J. Anim. Sci.

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. Ileal amino acid digestibility and estimates of endogenous amino acid losses in pigs fed rapeseed meal, sunflower meal and soybean meal. Can. J. Anim. Sci. 80: 495-506. Four commercial samples of rapeseed meal (RSM), four of sunflower meal (SFM) and nine of soybean meal (SBM) were analysed and their ileal N and amino acid (AA) digestibilities in growing pigs were studied. Each batch was tested on four castrated male pigs weighing between 40 and 100 kg and fitted with an end-to-end ileo-rectal anastomosis. True ileal digestibility (TD) of AA was calculated by correcting apparent ileal digestibility (AD) for basal endogenous AA losses, measured by means of a protein-free diet. For RSM and SBM, total endogenous gut AA losses were estimated by a multiple regression model and real ileal digestibility (RD) of AA was calculated. In RSM, average TD of lysine, threonine and methionine were 70.7, 73.2 and 86.0%, respectively. These values were 80.0, 81.8 and 91.9%, respectively in SFM and 90.5, 86.8 and 93.0%, respectively, in SBM. In SBM, TD decreased (P < 0.05) with increases in the ratio of dietary acid detergent fibre (ADF) to AA contents. Expressed as g kg -1 dry matter (DM) intake, total endogenous gut N losses reached 4.7 to 6.4 for RSM and 1.9 to 3.5 for SBM. For SBM, they increased (P < 0.001) with dietary ADF concentration and RD of most AA decreased (P < 0.05) with the increase in the ratio of dietary neutral detergent fibre (NDF) to AA concentrations.

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“…As the x 2 distance decreases, the similarity between the proteins increases. The proportions of dietary, endogenous and bacterial protein, which could be the main constituents of digesta protein, were assessed by the method developed by Duvaux et al (1990). This method uses a multiple regression analysis to establish the theoretical mixture which minimizes the x 2 distance with regard to the AA compositon of digesta.…”

Section: Chemical Analysesmentioning

confidence: 99%

Digestibility, blood levels of nutrients and skin responses of calves fed soyabean and lupin proteins

Tukur¹,

Pardal²,

Formal³

et al. 1995

Reprod. Nutr. Dev.

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Summary ― Three milk substitute diets in which the protein was provided either by skim milk only (control diet) or mainly (71%) by a commercial soyabean or lupin concentrate (soyabean or lupin diet, respectively) were given to intact or ileo-caecal-cannulated preruminant calves. In vitro tests showed that both concentrates were partially proteolysed and had low antigenic and antitryptic activities. The low antigenicity was confirmed in vivo since none of the calves produced specific plasma antibodies against dietary proteins, and skin reactions following the injection of these proteins were minor. Postprandial plasma level of triglycerides was higher with the 2 legume diets, suggesting faster abomasal emptying of fat and probably protein. Apparent faecal nitrogen digestibility was lower ( P 5 0.05) with the soyabean and lupin diets than with the control diet (0.86, 0.88 and 0.95, respectively). At the ileal level, the differences were smaller and non-significant (0.90, 0.88 and 0.92) for nitrogen, but remained significant for valine and tyrosine with the soyabean diet, and for proline, valine, methionine, leucine and lysine with the lupin diet. However, the differences were small enough to conclude that proper denaturation of soyabean and lupin proteins by processes including partial hydrolysis can suppress their antigenicity and render them very digestible.

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A new method of estimating the proportions of different proteins in a mixture using amino acid profiles: application to undigested proteins in the preruminant calf

Cited by 28 publications

References 7 publications

Digestibility and amino acid composition of digesta at the end of the ileum in preruminant calves fed soyabean protein

Digestibility and amino acid composition of digesta at the end of the ileum in preruminant calves fed soyabean protein

Ileal amino acid digestibility and estimates of endogenous amino acid losses in pigs fed rapeseed meal, sunflower meal and soybean meal

Digestibility, blood levels of nutrients and skin responses of calves fed soyabean and lupin proteins

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