A New Method for the Determination of Creatinine in Urine Samples Based on Disposable Pipette Extraction

Abstract

A method based on disposable pipette extraction (DPX) was successfully applied to creatinine determination in urine samples analysis using liquid chromatography with ultraviolet spectrophotometric detection (DPX/LC-UV). DPX variables, number of draw/eject cycles, sample pH, and type of the desorption solvent, were employed in a factorial experimental design to optimize the sorption equilibrium and time analysis. Among the evaluated DPX variables, the highest extraction efficiency was obtained with 500 µL of urine sample mixed with 1 mL of borate solution (pH 9) with one draw/eject cycle followed by liquid desorption of 1 mL of methanol in seven draw/eject cycles. The developed DPX/LC-UV method showed a linear response from the limit of quantification of 0.317 to 3.390 g L -1 with r 2 = 0.996 and inter-day precision with a coefficient of variation below 8.8%. Based on these results, the proposed method can be a useful tool for determining the creatinine levels in urine samples.

Dilute-and-Shoot HPLC-UV Method for Determination of Urinary Creatinine as a Normalization Tool in Mycotoxin Biomonitoring in Pigs

“…Reversed-phase high-performance liquid chromatography (RP-HPLC) [22] and liquid chromatography-tandem mass spectrometry (LC-MS/MS) [23][24][25] have been recently the most popular techniques for the measurement of creatinine in biological samples. They could be alternative techniques for creatinine determination with adequate sensibility and specificity.…”

Development of a reliable method for determination of N-nitrosamines in medicines using disposable pipette extraction and HPLC-MS analysis

“…The DPX technique exhibits advantages such as a high analyte transfer rate and reduced extraction time and demonstrates high analyte extraction and preconcentration efficiency and recovery. 15,17 Hence, this study aimed at the development and optimization of a sample preparation step based on the DPX technique for the analysis of NAs present in medicine of sartan class matrices. To the best of our knowledge, this is the inaugural application of DPX for NA extraction, which marks a pioneering advancement in the eld of pharmaceutical safety control.…”

“…The DPX technique exhibits advantages such as a high analyte transfer rate and reduced extraction time and demonstrates high analyte extraction and preconcentration efficiency and recovery. 15,17…”

Determination of Plant Growth Regulators in Chinese Herbal Medicine: A Comparison of Liquid (QuEChERS) and Solid (MSPD) Extraction Methods

“…Typically, there are two different kinds of extraction for rapid multi-component analysis using either liquid solutions or solid sorbents. 18 In recent years, the quick, easy, cheap, effective, rugged, and safe (QuEChERS) method using liquid…”