A new method for the cytological examination of the cerebrospinal fluid.

Sörnäs, Rune

doi:10.1136/jnnp.30.6.568

Cited by 70 publications

(13 citation statements)

References 11 publications

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“…There are several different methods for processing the CSF specimen for cytology (McCormick and Coleman, 1962;Bots et al, 1964;Enestrom, 1966;Sayk, 1966;Tourtellotte, 1966;Pariante and Scala, 1967;Sornas, 1967;Kola"r and Zeman, 1968;Sornas et al, 1972;Drewinko et al, 1973). Modifications of Sayk's (1966) sedimentation chamber and May-Griinwald Giemsa staining probably are the most common.…”

Section: Discussionmentioning

confidence: 99%

Differential cellular increase in cerebrospinal fluid after encephalography in mentally retarded patients

Iivanainen

Taskinen

1974

Journal of Neurology, Neurosurgery & Psychiatry

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SYNOPSISThe behaviour of the CSF cells during gas encephalography (GEG) with 2, N20, and halothane is poorly known in cerebral developmental disorders. One hundred and fifty CSF samples taken during pneumoencephalography from 75 mentally retarded patients were examined cytologically by the millipore technique with Papanicolaou staining. The results were processed automatically. An approximately 25-fold increase in CSF cells (P < 0-001), mainly meningeal, reticulohistiocytic, and monocytic types, was found to occur. The cortical gas filling rate had a positive correlation (P < 0-001) with the increase of number of CSF cells. There were no significant differences between the cellular changes in different cerebral disorders. Thus, though the irritant effect of GEG gives a rich cell yield, diagnostic atypical cells in developmental disorders of the central nervous system probably rarely exfoliate into the lumbar CSF.

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Section: Discussionmentioning

confidence: 99%

Differential cellular increase in cerebrospinal fluid after encephalography in mentally retarded patients

Iivanainen

Taskinen

1974

Journal of Neurology, Neurosurgery & Psychiatry

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“…Twenty millilitres of CSF was sampled into siliconized glass tubes. CSF cells were counted by phase-contrast microscopy [23]. Specimens with erythrocyte counts >5OxlOVi were excluded.…”

Section: Methodsmentioning

confidence: 99%

Multiple Sclerosis: Cells Secreting Antibodies Against Myelin‐Associated Glycoprotein are Present in Cerebrospinal Fluid

et al. 1991

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We evaluated the B-cell response in cerebrospinal fluid (CSF) and blood by enumerating cells secreting antibodies to myelin-associated glycoprotein (MAG) and, for reference, to myelin basic protein (MBP), two myelin components which may constitute targets for autoimmune attack in multiple sclerosis (MS). Among 25 untreated MS patients, 12 had cells in CSF secreting anti-MAG IgG antibodies (mean value 1 per 1429 CSF cells) and three also had cells secreting anti-MAG antibodies of the IgM isotype but at lower levels. In CSF from 2 out of 10 MS patients examined, anti-MAG and anti-MBP IgG antibody-secreting cells were present concurrently. Antibody-secreting cells were less frequent in blood and bone marrow, reflecting compartmentalization to CSF. Anti-MAG antibody-secreting cells were found in CSF from only 1 out of 27 control patients. The intrathecal production of anti-MAG and anti-MBP antibodies may be important in the pathogenesis of MS.

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“…The method described here is also simpler and more practical. It is difficult to scrape off completely the petroleum jelly in the method of Sornas (1967), and this method has also been criticised as timeconsuming (Castleberry et al, 1975). Our method not only eliminates the procedure of applying and scraping off the petroleum jelly but also shortens the time considerably.…”

Section: Methodsmentioning

confidence: 99%

“…However, it is not easy to prepare a good smear because the cells are easily lysed after a short period and also are easily damaged by centrifugation, fixation, and concentration (S6rnas, 1967). The sedimentation method of S6rnas appears to be a much better method for both cell recovery and morphology (Sornas, 1967;Aronson et al, 1974). We have de-Address for correspondence and reprints:…”

mentioning

confidence: 99%