A new method for studying the binding and ingestion of zymosan particles by macrophages

Lombard, Y.; Gadrey, Jean; Makaya-Kumba, M.; Fonteneau, Paul; Poindron, P.

doi:10.1016/0022-1759(94)90018-3

Cited by 43 publications

(28 citation statements)

References 21 publications

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“…To investigate whether macrophages play a causative role in stimulating RGC survival and regeneration, we used several methods to stimulate monocytes without infringing on the lens. Zymosan, a yeast membrane suspension that activates the mannose and ␤-glucan lectin-binding site of the CR3 ␤ 2 -integrin receptors (Lombard et al, 1994;Xia et al, 1999), resulted in massive macrophage infiltration into the eye. This was accompanied by a dramatic increase in GAP-43 expression in RGCs and axon regeneration beyond the injury site.…”

Section: Discussionmentioning

confidence: 99%

“…Several methods were attempted to stimulate macrophages in the eye without puncturing the lens. These included injecting interferon-␥ (IFN-␥; Life Technologies, Gaithersburg, MD; 5000 U in 5 l; n ϭ 5) at a dosage sufficient to activate monocytes throughout the nervous system (Sethna and Lampson, 1991); or Zymosan (625 g in 5 l; Sigma; n ϭ 5), a yeast cell wall preparation (Stewart and Weir, 1989;Ross and Vetvicka, 1993;Lombard et al, 1994;Fitch et al, 1999). We also introduced activated macrophages, obtained from donor animals by injecting Ca 2ϩ -and Mg 2ϩ -free buffer containing 0.025% trypsin and 2 mM EDTA into the peritoneal cavity as described (Smith and Hale, 1997); after 3 min, the cavity was opened, and fluid was removed and added to DMEM (Sigma) containing 1% fetal bovine serum (Gemini Bio-Products, Calabasas, CA).…”

Section: Methodsmentioning

confidence: 99%

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Lens Injury Stimulates Axon Regeneration in the Mature Rat Optic Nerve

et al. 2000

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In mature mammals, retinal ganglion cells (RGCs) are unable to regenerate their axons after optic nerve injury, and they soon undergo apoptotic cell death. However, a small puncture wound to the lens enhances RGC survival and enables these cells to regenerate their axons into the normally inhibitory environment of the optic nerve. Even when the optic nerve is intact, lens injury stimulates macrophage infiltration into the eye, Mü ller cell activation, and increased GAP-43 expression in ganglion cells across the entire retina. In contrast, axotomy, either alone or combined with intraocular injections that do not infringe on the lens, causes only a minimal change in GAP-43 expression in RGCs and a minimal activation of the other cell types. Combining nerve injury with lens puncture leads to an eightfold increase in RGC survival and a 100-fold increase in the number of axons regenerating beyond the crush site. Macrophage activation appears to play a key role, because intraocular injections of Zymosan, a yeast cell wall preparation, stimulated monocytes in the absence of lens injury and induced RGCs to regenerate their axons into the distal optic nerve.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Lens Injury Stimulates Axon Regeneration in the Mature Rat Optic Nerve

et al. 2000

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“…Our present results employing primary cultures of cardiac cells showed the mannose receptor-mediated phagocytosis of zymosan A, a carbohydrate-rich cell wall preparation derived from S. cerevisiae. The uptake of zymosan by macrophages has been investigated in detail (Lombard et al, 1994), and it was demonstrated that this internalization is accompanied by secretion of the inflammatory mediator tumor necrosis-factor-α (Young et al, 2001). The mannose receptors, which are lectin-like receptors, are implicated in receptor-mediated endocytosis of several microorganisms (Stahl, 1998).…”

Section: Discussionmentioning

confidence: 99%

Endocytic Pathway in Mouse Cardiac Cells.

Soeiro

Mota

Batista

et al. 2002

Cell Struct. Funct.

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ABSTRACT. Primary cultures of heart muscle cells provide powerful tools for cardiac cell biological research that permits both physiological and biochemical approaches. In the present study we analyzed the endocytosis of cardiac cells and presented morphological characterization of the endocytic machinery using markers, which enabled us to follow the fluid-phase, receptor-mediated endocytosis and the internalization of large particles. Our results demonstrated the route of the internalized cargo to early endosomes followed or not by its discharge in the late compartments. We also confirmed the ability of cardiac muscle cells to ingest large particles such as the mannosylated ligand zymosan A, and even internalize whole eukaryotic cells such as the protozoan parasite Trypanosoma cruzi. Since endocytosis is involved in many important cellular functions, the present work contributes to the knowledge of possible additional roles played by cardiac muscle cells besides their well known ability to act as physically energetic cells in the body, constantly contracting without tiring.

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“…Macrophage-like cells were allowed to adhere to 75-cm 2 tissue culture flasks (Falcon, Oxnard, CA) at 37°C in an incubator in a humidified atmosphere of 5% CO 2 for 2 h. Nonadherent cells were removed by two washings with Dulbecco's PBS 0.02% EDTA (Eurobio) at room temperature. The remaining adherent cells were 95% macrophages by light microscopy and May-Grünwald-Giemsa staining (23).…”

Section: Preparation Of CMmentioning

confidence: 99%

Recruitment of STAT3 for Production of IL-10 by Colon Carcinoma Cells Induced by Macrophage-Derived IL-6

Herbeuval¹,

Lelièvre

Lambert³

et al. 2004

The Journal of Immunology

100

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The immunosuppressive cytokine IL-10 is associated with poor prognosis in colon cancer. Although macrophages are involved in antitumor defenses, production of IL-10 by tumor cells may permit malignant cells escape to cell-mediated immune defenses. To investigate interactions between macrophages and tumor cells in humans, we cultured macrophages isolated from patients and tested the effect of these macrophages on the production of IL-10 by several tumor cell lines. Macrophages were isolated from pleural effusions of patients with malignancy and from noncancer control patients. We demonstrated that culture supernatants of macrophages from both sources strongly stimulated IL-10 production by the three different human colon adenocarcinoma cell lines, Colo 205, Colo 320, and HT29. Recombinant IL-6, but not IL-10, TNF-α, and IFN-α, stimulated the secretion of IL-10 by colon tumor cells. mAbs against IL-6 and IL-6R prevented the effect of macrophage culture supernatants and of rIL-6, respectively, on the production of IL-10 by the three cell lines. Cocultures of macrophages and colon cancer cells showed that these tumor cells first stimulated macrophages to produce IL-6, which was then followed by IL-6-induced IL-10 production by colon cancer cells. Finally, we showed that IL-10 gene regulation was mediated by STAT3, which was phosphorylated after the binding of IL-6 to IL-6R. This is the first demonstration that IL-6, secreted by macrophages, can induce a STAT3-mediated IL-10 production by colon tumor cells.

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A new method for studying the binding and ingestion of zymosan particles by macrophages

Cited by 43 publications

References 21 publications

Lens Injury Stimulates Axon Regeneration in the Mature Rat Optic Nerve

Lens Injury Stimulates Axon Regeneration in the Mature Rat Optic Nerve

Endocytic Pathway in Mouse Cardiac Cells.

Recruitment of STAT3 for Production of IL-10 by Colon Carcinoma Cells Induced by Macrophage-Derived IL-6

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