A new method for rapid construction of a Pseudomonas sp. HF-1 bioaugmented system: Accelerating acylated homoserine lactones secretion by pH regulation

Wang, Meizhen; Zheng, Xin; Zhang, Kun; Ding, Yangcheng; He, Hongzhen; Shen, Dongsheng; Feng, Huajun

doi:10.1016/j.biortech.2014.06.098

Cited by 18 publications

(14 citation statements)

References 37 publications

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“…In the long-term operation of a bioaugmented system, the abundance of the bioaugmented strain might decline, which means there is a decrease in the ability to degrade the pollutants (20). In our previous study, by improving the fitness of the strain in the wastewater system, the bioaugmented strain in the wastewater system became more stable, thereby enhancing the degradation of pollutants (2). The long-term stability of bioaugmented bacterial populations is important to wastewater treatments that employ microorganisms to carry out pollutant removal.…”

Section: Resultsmentioning

confidence: 97%

“…These advantages include cost and efficiency (2). However, bioaugmented systems present several challenges, including a propensity of these systems to rapidly lose efficiency (3).…”

mentioning

confidence: 99%

“…In previous studies, we found that bacterial quorum sensing (QS) improved the biofilm formation of inoculated bacteria and accelerated their colonization in the system (2,9,10). Pseudomonas aeruginosa has two complete acyl-homoserine lactone (AHL) QS systems, LasIR and RhlIR.…”

mentioning

confidence: 99%

“…Several environmental factors have been shown to affect quorum sensing-regulated traits (16). For example, pH directly affects the stability of QS signals in bioaugmented systems (17), which can affect biofilm formation (2). Alkaline pH has also been shown to favor LasR Ϫ mutants under some growth conditions (18).…”

mentioning

confidence: 99%

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Nitrogen Source Stabilization of Quorum Sensing in the Pseudomonas aeruginosa Bioaugmentation Strain SD-1

Wang

Lai

Dandekar

et al. 2017

Appl Environ Microbiol

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Pseudomonas aeruginosa SD-1 is efficient at degrading aromatic compounds and can therefore contribute to the bioremediation of wastewater. P. aeruginosa uses quorum sensing (QS) to regulate the production of numerous secreted "public goods." In wastewater bioaugmentation applications, there are myriad nitrogen sources, and we queried whether various nitrogen sources impact the stabilities of both QS and the bacterial populations. In a laboratory strain of P. aeruginosa, PAO1, the absence of a nitrogen source has been shown to destabilize these populations through the emergence of QS mutant "cheaters." We tested the ability of SD-1 to grow in casein broth, a condition that requires QS for growth, when the nitrogen source with either NH 4 Cl, NaNO 3 , or NaNO 2 or with no added nitrogen source. There was great variability in susceptibility to invasion by QS mutant cheaters and, by extension, the stability of the SD-1 population. When grown with NH 4 Cl as an extra nitrogen source, no population collapse was observed; by contrast, twothirds of cultures grown in the presence of NaNO 2 collapsed. In the populations that collapsed, the frequency of social cheaters exceeded 40%. NaNO 3 and NaNO 2 directly favor QS mutants of P. aeruginosa SD-1. Although the mechanism by which these nitrogen sources act is not clear, these data indicate that the metabolism of nitrogen can affect the stability of bacterial populations, an important observation for continuing industrial applications with this species.IMPORTANCE Bioaugmentation as a method to help remediate wastewater pollutant streams holds significant potential to enhance traditional methods of treatment. Addition of microbes that can catabolize organic pollutants can be an effective method to remove several toxic compounds. Such bioaugmented strains of bacteria have been shown to be susceptible to competition from the microbiota that are present in wastewater streams, limiting their potential effectiveness. Here, we show that bioaugmentation strains of bacteria might also be susceptible to invasion by social cheaters and that the nitrogen sources available in the wastewater might influence the ability of cheaters to overtake the bioaugmentation strains. Our results imply that control over the nitrogen sources in a wastewater stream or selective addition of certain nitrogen sources could help stabilize bioaugmentation strains of bacteria.KEYWORDS quorum sensing, social cheating, bioaugmentation, long-term stabilization, inorganic nitrogen B ioaugmentation, the use of selected microbial strains in activated sludge to strengthen their abilities for pollutant removal (1), has significant advantages in the degradation of toxic and hazardous compounds. These advantages include cost and

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Section: Resultsmentioning

confidence: 97%

“…These advantages include cost and efficiency (2). However, bioaugmented systems present several challenges, including a propensity of these systems to rapidly lose efficiency (3).…”

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Nitrogen Source Stabilization of Quorum Sensing in the Pseudomonas aeruginosa Bioaugmentation Strain SD-1

Wang

Lai

Dandekar

et al. 2017

Appl Environ Microbiol

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“…In addition, the reduced c-di-GMP level would destabilize biofilms and cause massive cell dispersal, which may eventually lead to bioaugmentation failure. For example, in real-world bioaugmentation cases, the augmented organism with weak biofilm formation ability resulted in a failed bioaugmentation (55), while enhanced biofilm formation capability improved the bioaugmentation performance (56). As a decreased c-di-GMP level leads to less initial attachment and biofilm dispersal, approaches to elevating or maintaining a high c-di-GMP level may be promising to establish and maintain sustainable bioaugmentation activity.…”

Section: Resultsmentioning

confidence: 99%

Influence of 3-Chloroaniline on the Biofilm Lifestyle of Comamonas testosteroni and Its Implications on Bioaugmentation

Mohanty

Chia

et al. 2016

Appl Environ Microbiol

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Bioaugmentation has been frequently proposed in wastewater and soil treatment to remove toxic aromatic compounds. The performance of bioaugmentation is affected by a number of biological and environmental factors, including the interaction between the target pollutant and the augmented bacterial cells. In this study, using Comamonas testosteroni and 3-chloroaniline (3-CA) as the model organism and target pollutant, we explored the influence of toxic aromatic pollutants on the biofilm lifestyle of bacteria capable of degrading aromatic compounds toward a better understanding of cell-pollutant interaction in bioaugmentation. Our results showed that the exposure to 3-CA greatly reduced the retention of C. testosteroni cells in packed-bed bioreactors (from 22% to 15% after three pore volumes), which could be attributed to the altered bacterial motility and cell surface hydrophobicity. To further understand the molecular mechanisms, we employed an integrated genomic and transcriptomic analysis to examine the influence of 3-CA on the expression of genes important to the biofilm lifestyle of C. testosteroni. We found that exposure to 3-CA reduced the intracellular c-di-GMP level by downregulating the expression of genes encoding c-di-GMP synthases and induced massive cell dispersal from the biofilms. Our findings provide novel environmental implications on bioaugmentation, particularly in biofilm reactors, for the treatment of wastewater containing recalcitrant industrial pollutants. IMPORTANCEBioaugmentation is a bioremediation approach that often has been described in the literature but has almost never been successfully applied in practice. Many biological and environmental factors influence the overall performance of bioaugmentation. Among these, the interaction between the target pollutant and the augmented bacterial cells is one of the most important factors. In this study, we revealed the influence of toxic aromatic pollutants on the biofilm lifestyle of bacteria capable of degrading aromatic compounds toward a better understanding of cell-pollutant interaction in bioaugmentation. Our findings provide novel environmental implications on bioaugmentation for the treatment of wastewater containing recalcitrant industrial pollutants; in particular, the exposure to toxic pollutants may reduce the retention of augmented organisms in biofilm reactors by reducing the c-di-GMP level, and approaches to elevating or maintaining a high c-di-GMP level may be promising to establish and maintain sustainable bioaugmentation activity.A romatic compounds are widely used in chemical and pharmaceutical industries (1). These organic compounds contain chemically stable benzene ring structures, hence their degradation by naturally occurring microbial communities in the contaminated environments is often slow (2, 3). This is because the indigenous microbial community usually lacks efficient catabolic capabilities for these anthropogenic compounds. To efficiently remove these toxic compounds from contaminated water or soil, bioa...

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Nicotine-degrading microorganisms and their potential applications

Liu¹,

Chen³

et al. 2015

Appl Microbiol Biotechnol

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Nicotine-degrading microorganisms (NDMs) are a special microbial group which can use nicotine as the sole carbon and nitrogen source for growth. Since the 1950s, the bioconversion of nicotine by microbes has received increasing attention, and several NDMs have been identified, such as Arthrobacter nicotinovorans, Microsporum gypseum, Pellicularia filamentosa JTS-208, and Pseudomonas sp. 41. In recent years, increasing numbers of NDMs have been isolated and identified from tobacco plantation soil, leaf, and tobacco waste. Meanwhile, the metabolic pathway and degradation mechanism of nicotine have been elucidated in several NDMs, such as A. nicotinovorans, Agrobacterium tumefaciens S33, Aspergillus oryzae, and Pseudomonas putida S16. Moreover, several NDMs have been used in improving the quality of cigarettes, treating tobacco waste, and producing valuable intermediates of nicotine. Here, we summarize the diversity, phylogenetic analysis, and potential applications of NDMs.

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A new method for rapid construction of a Pseudomonas sp. HF-1 bioaugmented system: Accelerating acylated homoserine lactones secretion by pH regulation

Cited by 18 publications

References 37 publications

Nitrogen Source Stabilization of Quorum Sensing in the Pseudomonas aeruginosa Bioaugmentation Strain SD-1

Nitrogen Source Stabilization of Quorum Sensing in the Pseudomonas aeruginosa Bioaugmentation Strain SD-1

Influence of 3-Chloroaniline on the Biofilm Lifestyle of Comamonas testosteroni and Its Implications on Bioaugmentation

Nicotine-degrading microorganisms and their potential applications

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