A new method for rapid determination of carbohydrate and total carbon concentrations using UV spectrophotometry

Albalasmeh, Ammar; Berhe, Asmeret Asefaw; Ghezzehei, Teamrat A.

doi:10.1016/j.carbpol.2013.04.072

Cited by 538 publications

(288 citation statements)

References 49 publications

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“…To assess polysaccharide losses (solubilization) by our wall preparation procedure, we ground inflorescence segments in 20 mM MES buffer or 1% SDS, incubated overnight, precipitated the extract with ice-cold ethanol, and measured total sugar content of the precipitated material (Albalasmeh et al, 2013). Solubilized polysaccharide was ;5% of the total wall polysaccharide content and was the same for segments #1 to #4.…”

Section: Cell Wall Composition Analysismentioning

confidence: 99%

Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems

et al. 2017

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At early stages of Arabidopsis (Arabidopsis thaliana) flowering, the inflorescence stem undergoes rapid growth, with elongation occurring predominantly in the apical ;4 cm of the stem. We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering. Force-extension curves revealed a gradient, from high to low, in the plastic and elastic compliances of cell walls along the elongation zone, but plots of growth rate versus wall compliances were strikingly nonlinear. Neutron-scattering curves showed only subtle changes in wall structure, including a slight increase in cellulose microfibril alignment along the growing stem. In contrast, solid-state nuclear magnetic resonance spectra showed substantial decreases in pectin amount, esterification, branching, hydration, and mobility in an apical-to-basal pattern, while the cellulose content increased modestly. These results suggest that pectin structural changes are connected with increases in pectin-cellulose interaction and reductions in wall compliances along the apical-to-basal gradient in growth rate. These pectin structural changes may lessen the ability of the cell wall to undergo stress relaxation and irreversible expansion (e.g. induced by expansins), thus contributing to the growth kinematics of the growing stem.

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Section: Cell Wall Composition Analysismentioning

confidence: 99%

Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems

et al. 2017

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“…However, this method is less accurate for determining carbohydrates in very low concentrations. Besides, the chemical reagents can cause serious health problems such as being corrosive to skin, eyes, and some may even affect the respiratory system (Albalasmeh et al 2013). Highperformance liquid chromatography (HPLC) is highly efficient in the determination of carbohydrates in microalgae biomass.…”

Section: Extraction and Determination Of Nutritional Values Of Microamentioning

confidence: 99%

An overview of the utilisation of microalgae biomass derived from nutrient recycling of wet market wastewater and slaughterhouse wastewater

2017

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Microalgae have high nutritional values for aquatic organisms compared to fish meal, because microalgae cells are rich in proteins, lipids, and carbohydrates. However, the high cost for the commercial production of microalgae biomass using fresh water or artificial media limits its use as fish feed. Few studies have investigated the potential of wet market wastewater and slaughterhouse wastewater for the production of microalgae biomass. Hence, this study aims to highlight the potential of these types of wastewater as an alternative superior medium for microalgae biomass as they contain high levels of nutrients required for microalgae growth. This paper focuses on the benefits of microalgae biomass produced during the phycoremediation of wet market wastewater and slaughterhouse wastewater as fish feed. The extraction techniques for lipids and proteins as well as the studies conducted on the use of microalgae biomass as fish feed were reviewed. The results showed that microalgae biomass can be used as fish feed due to feed utilisation efficiency, physiological activity, increased resistance for several diseases, improved stress response, and improved protein retention.

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“…The carbohydrate content was determined by the sulfuric acid-UV method as follows (Albalasmeh et al 2013). A 1 ml aliquot of carbohydrate solution was mixed with 3 ml concentrated sulfuric acid in a test tube and vortexed for 30 s. The temperature of the mixture rose rapidly within 10-15 s after addition of sulfuric acid.…”

Section: Adsorption Experimentsmentioning

confidence: 99%

Revision of adsorption models of xyloglucan on microcrystalline cellulose

et al. 2016

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Interactions among cellulose, hemicellulose and pectins are important for plant cell wall assembly and properties and also for industrial applications of these polysaccharides. Therefore, binding of pectin and xyloglucan on microcrystalline cellulose was investigated in this experiment by adsorption isotherms, zeta potential and scanning electron microscopy (SEM). Analysis of three isotherm models (Langmuir, Freundlich and Fowler-Guggenheim isotherms) showed that the experimental adsorption isotherm was well described via the Fowler-Guggenheim model, which includes lateral interaction between the adsorbate. The adsorption isotherm and zeta potential measurement showed that at temperature 25°C only xyloglucan adsorbed on the microcrystalline cellulose. In case of xyloglucan on cellulose, the equilibrium was reached in about 3-4 h, and the kinetics of adsorption were well described by the multiexponential equation. Analysis of the model suggests that two steps can be distinguished: diffusion and reconformation in an adsorbed layer. No adsorption of pectin was observed in this study. SEM study showed that xyloglucan may prevent cellulose from aggregation.

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A new method for rapid determination of carbohydrate and total carbon concentrations using UV spectrophotometry

Cited by 538 publications

References 49 publications

Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems

Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems

An overview of the utilisation of microalgae biomass derived from nutrient recycling of wet market wastewater and slaughterhouse wastewater

Revision of adsorption models of xyloglucan on microcrystalline cellulose

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