A new method for quantifying the enzyme activity of DGKs

The role of N-terminal phosphorylation of DGK-θ

“…Among different PTMs, phosphorylation is one of the most widely observed and well-studied modifications ( 5 , 6 , 7 ). However, despite the popularity of PTMs, little is known about the role of PTMs on DGKs ( 8 ). Perhaps the most well-studied PTM of DGKs is phosphorylation.…”

“…Perhaps the most well-studied PTM of DGKs is phosphorylation. Seven out of ten DGKs have been shown to be phosphorylated ( 3 , 8 ), and these phosphorylations were shown to modulate the enzyme activity and membrane binding. For example, mutation of sites known to be phosphorylated on DGKγ to glutamic acid led to a much higher enzymatic activity ( 9 ).…”