A New Method for Phenotyping Red Blood Cells Using Microplates

Llopis, F.; Carbonell-Uberos, Francisco; Montero, M.; Bonanad, Santiago; Planelles, Dolores; Plasencia, I; Riol, C.; Planells, T.; Carrillo, C.; Miguel, Ana De

doi:10.1159/000031093

Cited by 3 publications

(2 citation statements)

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“…Techniques based on haemagglutination include the tube [7], slide [8], and microplate tests [9,10] and the column agglutination system [11,12]. Although highly accurate and specific, these techniques are expensive, requiring specialised equipment and trained personnel.…”

Section: Introductionmentioning

confidence: 99%

Red blood cell transport mechanisms in polyester thread-based blood typing devices

et al. 2015

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A recently developed blood typing diagnostic based on a polyester thread substrate has shown great promise for use in medical emergencies and in impoverished regions. The device is easy to use and transport, while also being inexpensive, accurate, and rapid. This study used a fluorescent confocal microscope to delve deeper into how red blood cells were behaving within the polyester thread-based diagnostic at the cellular level, and how plasma separation could be made to visibly occur on the thread, making it possible to identify blood type in a single step. Red blood cells were stained and the plasma phase dyed with fluorescent compounds to enable them to be visualised under the confocal microscope at high magnification. The mechanisms uncovered were in surprising contrast with those found for a similar, paper-based method. Red blood cell aggregates did not flow over each other within the thread substrate as expected, but suffered from a restriction to their flow which resulted in the chromatographic separation of the RBCs from the liquid phase of the blood. It is hoped that these results will lead to the optimisation of the method to enable more accurate and sensitive detection, increasing the range of blood systems that can be detected.

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Section: Introductionmentioning

confidence: 99%

Red blood cell transport mechanisms in polyester thread-based blood typing devices

et al. 2015

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“…Previous reports detailed the possibility of performing weak D typing with a microplate method, including direct agglutination in the presence of potentiators, antibody dilutions, a solid-phase adherence method, or strategies that employed a red cell (RBC) monolayer in the bottom of the well. [2][3][4][5][6] These methods, however, did not become popular in routine RBC typing.…”

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confidence: 99%

Detection of weak D with a fully automated solid‐phase red cell adherence system

et al. 2005

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Red Blood Cell Agglutination for Blood Typing Within Passive Microfluidic Biochips

2018

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Pre-transfusion bedside compatibility test is mandatory to check that the donor and the recipient present compatible groups before any transfusion is performed. Although blood typing devices are present on the market, they still suffer from various drawbacks, like results that are based on naked-eye observation or difficulties in blood handling and process automation. In this study, we addressed the development of a red blood cells (RBC) agglutination assay for point-of-care blood typing. An injection molded microfluidic chip that is designed to enhance capillary flow contained anti-A or anti-B dried reagents inside its microchannel. The only blood handling step in the assay protocol consisted in the deposit of a blood drop at the tip of the biochip, and imaging was then achieved. The embedded reagents were able to trigger RBC agglutination in situ, allowing for us to monitor in real time the whole process. An image processing algorithm was developed on diluted bloods to compute real-time agglutination indicator and was further validated on undiluted blood. Through this proof of concept, we achieved efficient, automated, real time, and quantitative measurement of agglutination inside a passive biochip for blood typing which could be further generalized to blood biomarker detection and quantification.

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A New Method for Phenotyping Red Blood Cells Using Microplates

Cited by 3 publications

References 3 publications

Red blood cell transport mechanisms in polyester thread-based blood typing devices

Red blood cell transport mechanisms in polyester thread-based blood typing devices

Detection of weak D with a fully automated solid‐phase red cell adherence system

Red Blood Cell Agglutination for Blood Typing Within Passive Microfluidic Biochips

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