A New Method for Phenotyping Red Blood Cells Using Microplates

Llopis, F.; Carbonell-Uberos, Francisco; Montero, M.; Bonanad, S.; Planelles, M.D.; Plasencia, I; Riol, C.; Planells, T.; Carrillo, C.; Miguel, Ana De

doi:10.1046/j.1423-0410.1999.7730143.x

Cited by 13 publications

(10 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…From January 2003 to April 2004, the presence of Wr a antigen in RBCs from blood samples of 110,000 healthy volunteer blood donors from Valencia, Spain, was studied by the method described by Llopis and coworkers 9 . A commercially available anti‐Wr a antiserum (Biotest AG, Dreiech, Germany) and an anti‐Wr a obtained in our laboratory were used for performing Wr a antigen typing.…”

Section: Methodsmentioning

confidence: 99%

Incidence of Wr^a antigen and anti‐Wr^a in a Spanish population

et al. 2005

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

Incidence of Wr^a antigen and anti‐Wr^a in a Spanish population

et al. 2005

View full text Add to dashboard Cite

show abstract

“…This points out to the need to phenotyping RBC units to one or several antigenic systems, since only ABO and Rh (D) are usually typed. The supply of phenotyped RBCs for patients with several RBC antibodies presents a difficult task to hospital blood banks and regional blood centers,[9] since the Malaysian human population is composed of a number of ethnicities, namely Malays (54%), Chinese (25.1%), Indians (7.5%), and other ethnic origins (13.1%). [10]…”

Section: Introductionmentioning

confidence: 99%

Red cell phenotyping of blood from donors at the National blood center of Malaysia

Musa

Ahmed

Hashim

et al. 2012

Asian J Transfus Sci

View full text Add to dashboard Cite

Background:Human blood groups are polymorphic and inherited integral structures of the red cell membrane. More than 300 red cell antigens have been identified and further categorized into 30 major discrete systems. Their distribution varies in different communities and ethnic groups.Aims:This work was set to determine the prevalence of red cell phenotypes in donors from the major ethnic groups in Malaysia, namely, Malays, Chinese, and Indians.Materials and Methods:The work utilized the dextran acrylamide gel technique in which four types of gel cards were used to identify the blood groups of 594 subjects collected at the National Blood Transfusion Centre, Malaysia.Results:Blood group O and CDe/CDe (R1R1) were the most common in all ethnic groups. The cde/cde (rr) was more prevalent amongst Indians. The rare phenotypes found were cDE/cDE(R2R2) and cDE/CDE(R2Rz). With the Lewis system, the distribution of Le(a-b+) was similar among the ethnic groups. The rarest phenotype Fy(a-b-) was discovered in two donors. Jk(a-b-) was found in seven Malays and in two Indians. In the MNSs system, MN was common in Malays and Chinese, while the MM was more common among Indians. The rare SS was found in 19 donors. Malay and Chinese subjects had high P1 Negative blood but Indians showed high P1 positive blood. Within the Kell System, the very rare KK type was found in six subjects.Conclusions:The results obtained serve as an established database for the distribution of red cell phenotypes based on the blood group systems of donors from the major ethnic groups in Malaysia.

show abstract

“…A few examples of the current techniques of blood grouping include dry instant blood typing plate 39. Microplate‐based techniques,40–42 and integrated microfluidic biochips are among other options of blood grouping 43. Recently, interesting progress has been made in the fabrication of low‐cost, disposable and easy to use paper‐based44 and thread‐based45 blood typing devices.…”

Section: Methodsmentioning

confidence: 99%