Objectives: Air displacement plethysmography (ADP) may provide a partial alternative to body density (B d ) and therefore body composition measurement compared to conventional hydrodensitometry (H d ) in children. As there are no evaluation studies of ADP in children, this study had a two-fold objective: to compare B d estimates by ADP and H d ; and to compare fat estimates by both ADP and H d to fat estimates by another reference method, dual energy X-ray absorptiometry (DXA). Setting: Obesity Research Center, St. Luke'saRoosevelt Hospital, New York, USA. Subjects: One hundred and twenty subjects (66 femalesa54 males) who ranged in age from 6 ± 86 y and in body mass index (BMI, kgam 2 ) from 14.1 ± 40.0 kgam 2 met study entry criteria. Study Design: Cross-sectional study of healthy children (age 19 y) and adult group for comparison to earlier studies. Each subject completed ADP, H d , and DXA studies on the same day. Only subjects with subjectivelyjudged successful H d studies were entered into the study cohort.Results: There was a high correlation between B d by ADP and H d (B d Hd 0.11 0.8966B d ADP; r 0.93, SEE 0.008 gacm 3 , P`0.0001), although the regression line slope and intercept differed signi®cantly from 1 and 0, respectively. Additional analyses localized a small-magnitude B d bias in the child (n 48) subgroup. Both ADP and H d %fat estimates were highly correlated (r b 0.9, P`0.0001) with %fat by DXA in child and adult subgroups. Bland ± Altman analyses revealed no signi®cant %fat bias by either ADP or H d vs DXA in either children or adults, although a bias trend (P 0.11) was detected in the child subgroup. Conclusion: With additional re®nements, the air displacement plethysmography system has the potential of providing an accurate and practical method of quantifying body fat in children as it now does in adults. Sponsorship: This study was in-part supported by NIH Grants RR00645, NIDDK 42618 and NIDDK 37352.