A New Method for Isolation and Fractionation of Complement Fixing Antigens from Plasmodium knowlesi

D'Antonio, L; Doenhoff, Albert E Von; Fife, Earl H.

doi:10.3181/00379727-123-31394

Cited by 16 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Aminco-French pressure cell (13). The red blood cells were ruptured at 1500-2000 psi and the parasites were separated, washed with MgCI2, and finally fragmented at 18,000-20,000 psi.…”

Section: Methodsmentioning

confidence: 99%

Alterations of red blood cell sodium transport during malarial infection

Dünn¹

1969

J. Clin. Invest.

115

View full text Add to dashboard Cite

A B S T R A C T Previous studies have suggested that malaria induces changes in erythrocytic membrane permeability and susceptibility to osmotic lysis. The present study investigated erythrocytic transport of sodium with cells from Rhesus monkeys infected with Plasmodium knowlesi. Red blood cell sodium concentration was significantly elevated in 37 parasitized animals (21.8 ±1.2 mM; mean +SEM), as compared to 23 control animals (10.0 +0.38 mM). The cellular sodium increased with the density of parasitemia and the cellular potassium decreased in proportion to the elevation of sodium. Nonparasitized as well as parasitized erythrocytes possessed this abnormality of cation metabolism. Effective chloroquine therapy reversed the changes over a period of 4 days.Active sodium outflux rate constants were depressed in animals with malaria (0.202 +0.012), as compared to controls (0.325 ±0.027). Passive sodium influx rate constants were higher in infected monkeys (0.028 +0.002) than in control animals (0.019 +0.002). The cross incubation of malarial plasma with normal red blood cells induced a 22% diminution in active sodium outflux but no changes were observed in sodium influx.It is concluded that malaria alters erythrocytic sodium transport in all erythrocytes. The elevated intracellular sodium concentration is the net result of decreased sodium outflux and increased sodium influx. The plasmodium organism or the affected host may produce a circulating substance that is deleterious to erythrocytic membrane cation transport.

show abstract

“…Aminco-French pressure cell (13). The red blood cells were ruptured at 1500-2000 psi and the parasites were separated, washed with MgCI2, and finally fragmented at 18,000-20,000 psi.…”

Section: Methodsmentioning

confidence: 99%

Alterations of red blood cell sodium transport during malarial infection

Dünn¹

1969

J. Clin. Invest.

115

View full text Add to dashboard Cite

show abstract

“…(A blue filter was used to intensify lipid streaks for photographic purposes.) D'Antonio et al (5) found little or no erythrocyte contamination present in purified antigen preparations. This observation was strengthened by the lack of phospholipids in the LF lipid extract since contaminating erythrocyte membrane ma- 3.1 4.4 a Number before the colon is the number of carbons present in the fatty acid; number after the colon is the number of double bonds present.…”

Section: Fig 3 Tlc Of Polar Lipids From Unheated (A-c)mentioning

confidence: 98%

Lipid composition and activity of a lytic factor isolated from Plasmodium berghei

Boyer¹,

D'Antonio²,

Schiavone³

1979

Infect Immun

View full text Add to dashboard Cite

A fraction was obtained from Plasmodium berghei which induced hemolysis of the erythrocytes of mice and hamsters. This fraction, called lytic factor (LF), was found to be composed of a large amount of lipid material. An examination of the lipids showed the major lipids to be monoglycerides, diglycerides, triglycerides, fatty acids, long-chain alcohol, sterol, sterol ester, sterol glycoside, and two cerebrosides. The most abundant component found in the LF was sterol ester, followed in order by cerebrosides, sterol, and sterol glycoside. Lytic activity was found to be lost when samples were boiled for 5 min. An examination of the lipid composition of LF before and after boiling showed changes which may be useful in studies on the mechanism of activity of this factor. The fatty acid composition of the total lipid fraction of LF was examined by gas-liquid chromatography. The major fractions were 18:1 and 16:0 in unheated LF and 16:0 in the heated LF.

show abstract

“…Rodents and primates have been variously vaccinated against malaria with crude membrane-containing plasmodial fragments (7,11,12), isolated membrane particles (7,(9)(10)(11) free of host cell contamination (3,13,22), and, most recently, a purified membrane subfraction prepared by sucrose density gradient centrifugation (10). However, final purification of the involved protective antigen(s) has been hampered by the absence of simple effective nondenaturing techniques for separating it from its insoluble carrier components.…”

mentioning

confidence: 99%

“…Plasmodial protective antigen(s) was solubilized from large parasite membrane-containing fragments (E) (7) separated from host erythrocytes (RBCs) by the French pressure cell technique (7,13). A 20% suspension of pooled, washed P. berghei NK65D-infected RBCs, with leukocytes removed by aspiration or passage through powdered cellulose (20), was passed through the French pressure cell at 800 lb/in2.…”

mentioning

confidence: 99%

Malaria vaccine antigen(s): detergent solubilization, partial isolation, and recovery of immunoprotective activity

D'Antonio¹,

Keshavarz-Valian²,

Alger³

1984

Infect Immun

View full text Add to dashboard Cite

Plasmodium berghei-infected erythrocytes were solubilized with the nonionic detergent n-octyl glucoside and membrane-containing P. berghei fragments with either n-octyl glucoside or the ionic detergent sodium deoxycholate. Unsolubilized material was separated by centrifugation, and the detergent was removed from the respective supernatants by gel filtration. Reaggregated components in the respective void volume eluates acted as a specific vaccine in recipient mice, resulting in a dose-related accelerated elimination of infection and increased survival after challenge with the homologous parasite.

show abstract

A New Method for Isolation and Fractionation of Complement Fixing Antigens from Plasmodium knowlesi

Cited by 16 publications

References 0 publications

Alterations of red blood cell sodium transport during malarial infection

Alterations of red blood cell sodium transport during malarial infection

Lipid composition and activity of a lytic factor isolated from Plasmodium berghei

Malaria vaccine antigen(s): detergent solubilization, partial isolation, and recovery of immunoprotective activity

Contact Info

Product

Resources

About