A new method for fast chitin extraction from shells of crab, crayfish and shrimp

Abstract

A new method for quick chitin isolation from the shells of crab, crayfish and shrimp is described. The main difference between the new method and the conventional method is two sodium hypochlorite (NaClO) treatments for 10 min each before the processes of demineralisation and deproteinisation. After the NaClO treatment, only 15 min is adequate for the demineralisation and 20 min for the deproteinisation processes. Newly extracted chitin from crab, crayfish and shrimp shells and commercial chitin were characterised using FT-IR, TGA, X-ray diffractometry and elemental analysis. From the results, it was observed that the chitins isolated with the new method and the commercial chitin had almost the same physicochemical properties. The advantage of the new method compared to traditional methods is the relatively rapid chitin extraction. When compared to the traditional chitin extraction method, the proposed method appears to be promising regarding its time and energy saving nature.

Extraction and characterization of biopolymers from exoskeleton residues of the amazon crab Dilocarcinus pagei

“…Commonly, the exoskeleton of crabs possesses a wide range of organic compounds, especially carotenoid pigments, such as astaxanthin (OGAWA et al, 2007). Several procedures for removal of the non-polymeric organic content involving mainly oxidizing compounds, such as potassium permanganate and sodium hypochlorite (LIU et al, 2012;KARAARSLAN, 2015) are described. It was observed that after the contact of the DP2 sample with NaClO solution, the resulting solid (QT3 sample) exhibited a light brown coloration (image not shown), evidencing the reduction of this organic fraction in the final material.…”

Isolation, screening and identification of haloarchaea with chitinolytic activity from hypersaline lakes of Iran

“…Due to the high number of strains screened in this investigation, extraction of chitin from shrimp shells was more cost-effective than the one purchased from Sigma-Aldrich. The process of chitin extraction from the shells of saline water shrimps was carried out according to the literature [23]. Shrimp shells were dried and ground.…”

“…Exochitinases break the chain from the ends, producing either chitobiose or N-acetylglucosamine [14]. They are present in 4 families (18,19,23, and 48) of glycosyl hydrolase (GH). The GH families are categorized based on amino acid sequence homology [15].…”

ChiBio: An Integrated Bio-refinery for Processing Chitin-Rich Bio-waste to Specialty Chemicals

“…Fermentation of minced scampi waste (Nephrops norvegicus) using Lactobacillus paracasei A3 and added glucose resulted in 77.5% DP and 61% DM after 5 days at 30 C, while the solid fraction contained 17.5% chitin (dry mass) [88]. Crayfish waste was also used in fermentation with L. paracasei and dextrose yielding 94% DP and 97% DM [89]. A mixture of proteolytic enzyme-producing bacteria (L. plantarum, L. salivarius, S. faecium, and P. acidilactici) was used for the anaerobic fermentation of prawn [90].…”