A New Method for Estimating Serum Acid Phosphatase of Prostatic Origin Applied to the Clinical Investigation of Cancer of the Prostate 12

Abstract

Since the studies of Gutman and Gutman (2, 3), measurements of the serum acid phosphatase have become a recognized and widely used ancillary procedure for the diagnosis of cancer of the prostate and a practical means for the evaluation of the functional status of the tumor in many patients with metastasizing cancer of the prostate. While an abnormally increased value of serum acid phosphatase is considered to be a specific index for the presence of carcinoma of the prostate, a normal serum acid phosphatase value in itself cannot be interpreted as signifying absence of this disease. According to Bodansky and Bodansky (4) Attempts have been made to render the determination of serum acid phosphatase more specific for cancer of the prostate. Thus, a search has been made for substrates which would be split prefer-

Methyl Jasmonate Ameliorates Testosterone Propionate-induced Prostatic Hyperplasia in Castrated Wistar Rats

“…Determination of acid and alkaline phosphatase activities. The activity of prostatic acid phosphatase (PAP) was determined by the method of Fishman et al (1953). Total acid phosphatase (A) and non-PAP (B) were obtained by assay experiments, while PAP was obtained by subtracting B from A. Alkaline phosphatase (ALP) activity was measured spectrophotometrically by monitoring the concentration of phenol formed when ALP reacts with disodium phenyl phosphate at 680 nm as described by Williamson, (1972).…”

Tartrate-Resistant Acid Phosphatase (TRACP): A Personal Perspective

“…Thus, to increase the specificity for prostate disease, acid phosphatase was measured as the tartrate‐sensitive fraction. The tartrate‐resistant fraction was believed to come primarily from red blood cells and was considered simply an interference (4) . However, it was also noted that patients with bone diseases, particularly cancer patients with lytic bone lesions, also had increased acid phosphatase activity in the serum, and this acid phosphatase was resistant to inhibition by tartaric acid (5) .…”

A Status Report – Human Prostatic Carcinoma, with Emphasis on Potential for Viral Etiology

“…Li, Kanwal and Kim [53] state that prostatic epithelium produces an ''external" SAP, and that carcinoma of the prostate elaborates excessive amounts available to the circulation; mithramycin and dactinomycin, inhibitors of cellular RNA synthesis, can significantly decrease, transiently, the SAP levels in drugadministered patients with wide spread CAP. Prostatic SAP may be L-tartrate labile [54,55] but a later study questioned this specificity [56], Several studies [57][58][59][60] concluded that assessment of bone marrow SAP levels independent of tissue source is of use in diagnostic staging of all patients. Li et al [61] and R e if et al [62] have recently demonstrated significant electrophoretic differences between the isoenzymes contributing to the plasma and serum acid phosphatase levels in normal and pathologically involved human patients.…”