A New Method for Elution of Erythrocyte-Bound Antibody

Abstract

A new method is described for eluting antibody from erythrocytes. Sensitised erythrocytes are subjected to ultrasonic waves; elution probably depends on the application both of heat and great mechanical force. It was successfully used to elute human and animal IgG and IgM antibodies and seed agglutinins. By direct application of ultrasound to sensitised erythrocytes the time taken to produce strong eluates ranged from 30 sec for some seed agglutinins to 10 min for IgG anti-D.

Erythrocyte Membrane Modification in Malignant Disease of Myeloid and Lymphoreticular Tissues

“…Furthermore, the unagglutinated cells were found not to be of group 0 but a very weak form ofB, so weak that these cells were not agglutinated by anti-B reagents, even hyperimmune anti-B. The cells, however, did take up anti-B, which could be recovered from the red cells by the ultrasonic technique of Bird & Wingham (1972) which we find to be particularly satisfactory for the elution of antibodies taken up by RBC in vitro. The H, I and i antigens of both cell populations were normal.…”

Quantification of Platelet-Bound Alloantibodies by Radioimmunoassay: A Study on Some Variables

“…Because these fragments are not (accurately) counted, but contribute to the amount of IgG in the test suspension, their presence could lead to false elevated PA-IgG values, as we found. An alternative explanation for our results shown in table IV may be the fact, well known from red cell serology [Bird and Wingham, 1972], that platelet antibodies may have been eluted due to the sonication procedure. However, this explanation seems not likely, because in our experiments the time period between the moment of addition of the fragmentated cells to the PRP and the further washings was too short to allow supposedly eluted antibodies to (re-)bind to intact platelets.…”

The M, N and N(vg) Receptors of Tn-Erythrocytes

“…Normal erythrocytes of appropriate MN groups were sensitised with human and rabbit anti-M sera and rabbit anti-N serum by conventional methods, and eluates pre pared by the ether [9] and ultrasonic [3] techniques. The eluates which contain anti-M or anti-N, without anti-Tn, were titrated against separated Tn and 'non-Tn' cells by con ventional twofold dilution methods and the results scored as previously described [2], Vicia graminea seed extract (anti-Nvg) was prepared in the same way as the soya bean extract and tested in the same way as the anti-M and anti-N eluates.…”