A NEW METHOD FOR DETERMINATION OF TERT‐BUTYLHYDROQUINONE (TBHQ) IN RBD PALM OLEIN WITH FTIR SPECTROSCOPY

Abstract

Fourier transform infrared (FTIR) spectra of refined bleached and deodorized (RBD) palm olein samples between 3600 and 2800 cm−1 were used for quantitative determination of the content of tert‐butylhydroquinone (TBHQ). The method was based on sodium chloride (NaCl) windows with transmission path fixed at 50 µm at room temperature. Fifty stripped oil samples spiked with known amounts of TBHQ concentrations up to 300 mg/kg (ppm) were separated into two sets for calibration and validation models based on partial least squares (PLS) analyses. The accuracy of the method was comparable to that of the method established by the International Union of Pure and Applied Chemistry (IUPAC) with a coefficient of determination (R2) of 0.9961 and standard error of calibration of (SEC) 5.06. The model was validated, and the R2 of validation and standard error of prediction were 0.9951 and 6.49, respectively. The standard deviation of difference for repeatability for the method was comparable to that for the IUPAC method. The new FTIR method developed for determining TBHQ in palm olein was demonstrated to be efficient, accurate, rapid and required minimum solvent as only acetone was used for cleaning NaCl windows. The method is suitable for routine quality control analysis.

Migration analysis, antioxidant, and mechanical characterization of polypropylene‐based active food packaging films loaded with BHA, BHT, and TBHQ

“…Moreover, wavenumber of 1190 cm −1 was assigned to gem‐dimethyl group, characteristic of O—C aromatic vibration. Finally, peaks at 771 and 692 cm −1 represented out of plane —CH and phenol ring bending (Ammawath, Man, Baharin, & Rahman, 2005; Pavia et al., 2008).…”

CUPRAC total antioxidant capacity assay of lipophilic antioxidants in combination with hydrophilic antioxidants using the macrocyclic oligosaccharide methyl β-cyclodextrin as the solubility enhancer

“…3 depicts the FT-IR spectra of solid inclusion complexes of TBHQ with M-b-CD obtained by various methods. The TBHQ spectrum exhibited 3445 and 3272 cm À1 bands for -OH associated in phenol, 2963 cm À1 were assigned as unsaturated @CH-(as in aromatics), 1590, 1484 and 1442 cm À1 were due to phenyl ring stretching and -C-OH in-plane bending, 1366 and 1310 cm À1 for methylene and methyl bending vibration, 1209 and 1184 cm À1 for gem-dimethyl group (i.e., two methyl groups of the same carbon atom), characteristic of O-C aromatic vibration, and 808, 771 and 692 cm À1 for out-ofplane -CH and ring [29]. The characteristic bands of TBHQ were again obscured in the 2000-500 cm À1 range, in the kneaded and freeze-dried products, verifying complex formation with M-b-CD.…”

Determination of Tertiary‐butylhydroquinone and Its Metabolites in Rat Serum by Liquid Chromatography–Ion Trap Mass Spectrometry

“…To evaluate the safety of TBHQ, several methods have been carried out for the analysis of TBHQ in body fluids as well as foods including Fourier transform infrared (FTIR) spectroscopy, colorimetry, UV-visible photometry, electroanalytical-voltammetry, paper and thin-layer chromatography, gas and liquid chromatography [7][8][9][10][11]. Most of these methods require long analysis times and laborious sample treatment techniques in addition to low resolution.…”