A new method for delivering alkanes to mammalian cells: preparation and preliminary characterization of an inclusion complex between β-cyclodextrin and pristane (2,6,10,14-tetramethylpentadecane)

Janz, Siegfried; Shacter, Emily

doi:10.1016/0300-483x(91)90189-8

Cited by 26 publications

(25 citation statements)

References 36 publications

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“…Cells were grown in DMEM supplemented with 10% FBS under standard cell culture conditions. Due to its insolubility in aqueous medium, pristane (Sigma-Aldrich) was added as a suspension of inclusion complexes with ␤-cyclodextrin (␤-CyD; Sigma-Aldrich) as previously described (6).…”

Section: Cells and Culture Conditionsmentioning

confidence: 99%

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Induction of Apoptosis by the Hydrocarbon Oil Pristane: Implications for Pristane-Induced Lupus

Calvani

Caricchio

Tucci

et al. 2005

The Journal of Immunology

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Intraperitoneal injection of the hydrocarbon oil pristane into normal mice leads to a lupus-like autoimmune syndrome. Although advances in defining the roles of cellular and humoral mediators involved in this syndrome have been made, the mechanisms that initiate a break in tolerance leading to autoimmunity remain unknown. We describe in this study that pristane induces apoptosis both in vivo and in vitro. Pristane arrests cell growth and induces cell death by apoptosis via the mitochondrial pathway of caspase activation in a dose-dependent manner. Nuclear autoantigens created by pristane-induced apoptosis of lymphoid cells within the peritoneal cavity in the setting of a profoundly altered cytokine milieu may be the initiating event in the development of autoimmunity in this syndrome. These findings suggest that apoptosis may be a critical initial event in the pathogenesis of pristane-induced lupus and are of potential relevance for human systemic lupus erythematosus.

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Section: Cells and Culture Conditionsmentioning

confidence: 99%

“…Pristane is a membrane-active compound that has been shown to interact with phospholipid bilayers and cell membranes. It has been reported to have a cytotoxic effect dependent on concentration and cell line (6), although the mechanism of this cytotoxicity remains unknown.…”

mentioning

confidence: 99%

Induction of Apoptosis by the Hydrocarbon Oil Pristane: Implications for Pristane-Induced Lupus

Calvani

Caricchio

Tucci

et al. 2005

The Journal of Immunology

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“…Because of its insolubility in aqueous medium, TMPD was added as the inclusion complexes with b-cyclodextrin (Wako) as described previously (24,25).…”

Section: Culture Of Peritoneal Cellsmentioning

confidence: 99%

Essential Requirement for IFN Regulatory Factor 7 in Autoantibody Production but Not Development of Nephritis in Murine Lupus

Miyagawa

Tagaya

Ozato

et al. 2016

The Journal of Immunology

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Systemic lupus erythematosus (SLE) is a prototypic systemic autoimmune disease characterized by the production of autoantibodies against nuclear components. Recent genetic studies of SLE patients have revealed that IFN regulatory factor (IRF) 7 gene polymorphisms are associated with an increased risk of SLE, but the precise role of IRF7 in SLE development is not fully understood. We investigated the role of IRF7 in the pathogenesis of SLE using a mouse model and saw a curious dissociation of autoantibody production and development of glomerulonephritis. SLE was chemically induced into IRF7-deficient mice, and glomerulonephritis with deposits of IgG and lipogranulomas were observed after 10 mo. However, these mice failed to produce anti-dsDNA, ssDNA, ribonucleoprotein, and Sm autoantibodies. Following the chemical induction, IRF7-deficient mice expressed substantially lower levels of IFN-stimulated genes than did wild-type mice, but NF-κB target genes were equally upregulated in both strains. Therefore, the type I IFN pathway seems critical for the autoantibody production, but the NF-κB activation is sufficient for the development of glomerulonephritis in this model. Our study thus demonstrates a specific requirement for IRF7 in autoantibody production and uncovers a new layer of complexity in the pathogenesis of SLE.

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“…CDs have been utilized for this purpose. Low concentrations (∼0.2%) of CDs have been employed to solubilize and deliver extremely hydrophobic substances to cells in culture (Janz and Shacter, 1991;Vicanova et al, 1999;Pfitzner et al, 2000). Higher concentrations (∼20%) of CD have also been used to solubilize hydrophobic drugs for delivery through intracerebral and intrathecal routes (Yaksh et al, 1991;Jang et al, 1992;Yamamoto and Yaksh, 1992).…”

Section: Discussionmentioning

confidence: 99%

Characterization of methyl-β-cyclodextrin toxicity in NGF-differentiated PC12 cell death

et al. 2007

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Cyclodextrins (CDs) are used to deliver hydrophobic molecules in aqueous environments. Methyl-β-cyclodextrin (MβCD), a member of this family of molecules, has been proposed to be a good carrier to deliver fatty acids to cells in culture. This report focuses on studying the in vitro effects of MβCD on nerve growth factor-differentiated PC12 (NGFDPC12) cells, a tissue culture model to study neuronal survival and differentiation. The main findings are: (1) NGFDPC12 cells have normal viability when exposed to 0.12% MβCD but showed a significant loss in cell viability at higher concentrations; (2) NGFDPC12 cells exposed to 0.25% MβCD exhibit nuclear condensation, blebbing and apoptotic bodies, and whole cell lysates exhibited an increase in caspase-3-like activity and high levels of Bax and Bcl-X L protein expression compared to control. Cultures treated with 0.25% MβCD also showed cleavage of normal 21-kDa Bax protein into a 18-kDa fragment. (3) Experiments using 0.12% MβCD to deliver oleic acid did not affect cell viability, in contrast NGFDPC12 cultures in which 0.25% MβCD concentration is used exhibited similar loss of cell viability as observed with 0.25% MβCD alone. Treating these cultures with caspase-3 inhibitor z-VAD-fmk did not protect the cells from MβCD toxic effects. (4) Immortalized Schwann cells (iSC) exposed to MβCD 0.12% did not show loss of cell viability while 0.25% MβCD triggered a significant toxicity but with a different dose and time course dynamic than NGFDPC12 cells. Thus, NGDPC12 or iSC cell cultures exposed to 0.12% MβCD exhibits normal viability while higher concentrations increase in cell death and apoptosis.

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A new method for delivering alkanes to mammalian cells: preparation and preliminary characterization of an inclusion complex between β-cyclodextrin and pristane (2,6,10,14-tetramethylpentadecane)

Cited by 26 publications

References 36 publications

Induction of Apoptosis by the Hydrocarbon Oil Pristane: Implications for Pristane-Induced Lupus

Induction of Apoptosis by the Hydrocarbon Oil Pristane: Implications for Pristane-Induced Lupus

Essential Requirement for IFN Regulatory Factor 7 in Autoantibody Production but Not Development of Nephritis in Murine Lupus

Characterization of methyl-β-cyclodextrin toxicity in NGF-differentiated PC12 cell death

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