A New Method for Cultivation of Sclerotium ofGrifola umbellata

Abstract

Sclerotia of Grifola umbellata were cultivated by two methods such as burying and root inoculation methods. The sclerotia of G. umbellata produced by the burying method were 6.0~6.8 × 3.4~4.6 × 1.8~1.9 cm (Width × Length × Thickness) in size and 17.3~19.6 g in weight, respectively. Their increase rate was 1.10~1.12 times. On the other hand, the sclerotia cultivated by the root inoculation method were 18.3~31.5 × 12.5~26.4 × 3.1~3.7 cm (W × L × T) in size and 219.1~576.6 g in weight, respectively. Their growth increment was 11.18~39.77 times. The rhizomorphs of Armillaria mellea were developed with a high density under fallen leaves layer covering cultivation site, and distributed mainly between soil surface and soil depth of about 10 cm as well as colonized prominently on the inoculated wood logs. Fungal interaction between G. umbellata and A. mellea were observed mainly in the stage of white sclerotium of G. umbellata. The sclerotia of G. umbellata which were developed newly and harvested in the root inoculation method were twined with root hairs of host tree and rhizomorphs of A. mellea. The sclerotia of G. umbellata decomposing root hairs of host tree were confirmed through SEM examination. Physiochemical characteristics of soil in all cultivation sites had no significant differences. Soil pH were in the range of pH 3.98~4.40. Organic matters were the range of 17.97~23.86% and moisture contents of soil were 12.00~18.20%. Soil temperatures showed 12.9~13.8 o C in November and 22.0~23.9 o C in August, respectively. In conclusion, the root inoculation method seems to be a practical method for cultivating sclerotia of G. umbellata due to its many advantages such as simplicity of inoculation process, shortening of cultivation periods and facility of harvest.

Sclerotial Formation of Polyporus umbellatus by Low Temperature Treatment under Artificial Conditions

“…Fr., one of the most precious and widely used medicinal fungi, belong to the Polyporaceae family of Basidiomycota phylum [1]–[2]. P. umbellatus sclerotia have been shown to possess pharmacological activities for the treatment of conditions such as acute nephritis and edema [3].…”

The Optimal Culture Conditions for the Mycelial Growth ofOudemansiella radicata

“…), one of edible and medicinal mushrooms belonging to Tricholomataceae of Agaricales has been known to be inhabited on the soil surface or rotted woods located in the broad-leaved forest, mixed forest of broad-leaved and needle-leaved trees for the duration of summer to autumn (Lee, 1988). Many mushroom researchers (Shim et al, 1997; Ha, 2001; Choi et al, 2003; Lee et al, 2004) have been worked to find substances to protect human health from intractable diseases such as a cancer, gastric ulcer and hypertension and so on. Oudenone, one of medicinal substance isolated from fruiting bodies of O. radicata , has been known to exhibit outstanding therapeutic effects on the hypertension caused by high blood pressure and inhibitory effect on Pyricularia oryzae which caused rice blast disease (Park and Lee, 1999; Ying et al, 1987).…”

Quantitative Analysis of Polysaccharide Composition in Polyporus umbellatus by HPLC–ESI–TOF–MS

“…Polyporus umbellatus (Pers.) Fries, also known as “Zhu Ling” in China, belongs to the Polyporaceae family of the Basidiomycota phylum [1,2]. It is a widely used medicinal fungus in Asia, especially in China and Japan, and has been one of the most important traditional Chinese medicines for more than 2500 years [3].…”