A New Method for Continuous Chronic Measurement and Recording of Blood Pressure, Heart Rate and Activity in the Rat Via Radio-telemetry

Brockway, Brian; Mills, Perry; Azar, Silvia

doi:10.3109/10641969109042094

Cited by 198 publications

(109 citation statements)

References 5 publications

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“…Radiotelemetric blood pressure monitoring was used to assess 24-h blood pressure control [24]. Male SHR, aged eight weeks, weighing between 180 to 220 g were synchronized to 12:12 h of light (07.00-19.00) and dark (19.00-07.00) for 5 days.…”

Section: Methodsmentioning

confidence: 99%

Renoprotective effects of vasopeptidase inhibition in an experimental model of diabetic nephropathy

et al. 2003

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Aims. Although ACE inhibitors slow progression of diabetic renal disease, the mortality and morbidity is still high. As other hormonal factors are involved, inhibition of vasopeptidases could further reduce progression. We studied dual inhibition of angiotensin converting enzyme and neutral endopeptidase in a model of progressive diabetic renal injury. The major endpoints were reductions in systemic blood pressure, albuminuria and renal structural injury. Methods. Diabetic spontaneously hypertensive rats were treated with the ACE inhibitor perindopril (mg·kg −1 · day −1 ) or the vasopeptidase inhibitor omapatrilat at doses of 10 (oma10) and 40 (oma40) mg·kg −1 ·day −1 for 32 weeks. In vivo ACE and NEP inhibition was quantitated by in vitro autoradiography. Renal structural injury was assessed by measurement of the glomerulosclerotic (GS) index and tubulointerstitial area (TI). The expression of transforming growth factor β, β-inducible geneh3 and nephrin were also quantitated.Results. Despite a similar reduction in blood pressure by perindopril and oma10, greater attenuation of albuminuria was afforded by oma10, with a complete amelioration observed with oma40. Oma40 lead to a 33% reduction in renal NEP binding and this was associated with less albuminuria and prevention of GS, TI area and overexpression of TGFβ and βig-h3. Diabetes-associated reduction in nephrin expression was restored by both drugs. Conclusion/Interpretation. These findings suggest that other vasoactive mechanisms in addition to angiotensin II are important in the prevention of diabetic nephropathy, and that vasopeptidase inhibition might confer an advantage over blockade of the RAS alone in the treatment of diabetic renal disease. [Diabetologia (2003) 46:961-971]

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Section: Methodsmentioning

confidence: 99%

Renoprotective effects of vasopeptidase inhibition in an experimental model of diabetic nephropathy

et al. 2003

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“…It provides the ability to collect data around the clock without the stress from human contact. 7 We have previously used a radio-telemetry system (Dataquest IV, Data Sciences International) to monitor blood pressure and heart rate in genetic crosses designed to map quantitative trait loci for blood pressure regulation. 2,8 However, these studies used simple means and standard deviations of blood pressure and heart rate variables measured over 96 hours at baseline or after salt-loading.…”

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confidence: 99%

Telemetry for Cardiovascular Monitoring in a Pharmacological Study

et al. 1999

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Abstract-Radio-telemetry systems offer the ability to measure blood pressure and heart rate in experimental models of hypertension without the stress artifacts induced by some other methods. We therefore aimed to develop improved, nonparametric regression methods for radio-telemetry data and to use these to assess the effects of pharmacological interventions on cardiac and vascular hypertrophy in the stroke-prone spontaneously hypertensive rat. One control group and 5 groups treated either with losartan (alone or in combination with N G -nitro-L-arginine methyl ester [ L-NAME]), perindopril (also alone or in combination with L-NAME), or hydralazine plus hydrochlorothiazide were monitored for 4 weeks. Cardiac hypertrophy was assessed by the left ventricle plus septum weight to body weight ratio and vascular hypertrophy by flow-cytometry analysis of vascular smooth muscle cell polyploidy. Hemodynamic series were split into trend and cyclic components by the seasonal and trend decomposition procedure based on Loess and compared between groups by Loess regression modeling. Systolic and diastolic blood pressures were reduced systematically by losartan and perindopril (PϽ10 Ϫ10) but to a lesser extent by hydralazine plus hydrochlorothiazide (PϽ10 Ϫ8 ), and diurnal variation was reduced in the latter group (PϽ10 Ϫ6). L-NAME significantly reduced the hypotensive effect only of losartan. Vascular and cardiac hypertrophy were significantly attenuated with losartan or perindopril, but were unchanged with other treatments. The new analysis proposed here identifies differential effects on trends and cyclic variation and associations with regression of end-organ damage for losartan and perindopril compared with hydralazine plus hydrochlorothiazide. The method offers a powerful tool for detailed investigation of radio-telemetry data. Key Words: telemetry Ⅲ statistics Ⅲ hypertension, genetic Ⅲ hypertrophy, cardiac Ⅲ rats M onitoring of blood pressure and heart rate in experimental models of hypertension has played an important part in the study of cardiovascular physiology, pharmacology, and more recently in the genetics of hypertension. 1Ϫ3 A wide variety of techniques have been used to obtain these measurements. Anesthesia is the simplest method but it affects normal physiological regulation and thus measurements from conscious animals are preferred. 4 The most common techniques for monitoring conscious animals use tethers or restraining devices with measurements taken via indwelling exteriorized vascular catheters or blood pressure cuffs. 5 This use of restraints and tethers introduces a significant stress artifact with well-documented elevations of plasma catecholamines and cortisol. 6 In addition, the so called noninvasive tail-cuff measurements require both warming and restraint, thus producing several stressful stimuli.Radio-telemetry provides an alternative means of obtaining blood pressure and heart rate measurements from freely moving conscious animals. It provides the ability to collect data around the clock without ...

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“…Radiotelemetry transmitter installation surgery was carried out at 13-14 weeks of age under methohexital anesthesia for an abdominal incision to place an aortic catheter, affixing transmitters to the abdominal wall. The accuracy of telemetry SBP readings was verified by a standard mercury sphygmomanometer [1,[29][30][31][32][33]. Rats were returned to their home cage when they were fully awake and mobile with freely accessible food and acetaminophen in 5% glucose solution for the first 24 hours post-surgery, followed by tap water.…”

Section: Telemetry Transmitter Implants and Measurements Of Blood Prementioning

confidence: 99%

Effects of Perinatal Protein-Nacl Diets on Offspring's Blood Pressure and Renal Function in Lewis Rats

Lee¹,

Azar²

2013

TOHYPERJ

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Perinatal diets may affect the cardiovascular-renal functions of offspring. To understand effects of maternal diet on the renal function and blood pressure (BP) of offspring, protein (10% low, LP; 23% normal, NP) and/or NaCl (4% high salt, HS; 0.6% normal, NS) diets were started at pre-pregnancy through pups' weaning to either a 4% high NaCl (hs) or 0.6% NaCl (ns) diet. Telemetered BP data was analyzed by methods of linear least square rhythmometry. Systolic BPs (circadian mean ±SE mm Hg) were: NPNSns, 131±2; NPNShs, 137±2; NPHSns, 137±0.2; NPHShs, 134±3; LPNSns, 138±1; LPNShs, 138±0.6; LPHSns, 135±2; LPHShs, 142±2. Offspring in NPNShs and NPHSns had significantly increased SBPs versus NPNSns (both P<0.05). Most LP-offspring had increased SBP (P<0.01 to <0.05) and lower body weight (BW) with smaller glomerular filtration rate changes (renal reserve, RR-GFR) following overnight acute highprotein loads: RR-GFRs (inulin, ml/min/g kidney) for groups stated above were, respectively: 0.935; 0.927; 0.537; -0.064; -0.229; 0.057; -0.515; -0.404. The kidney weight/BW ratio of offspring was higher on hs-than on ns-diets (all P<0.001). Rats on a low caloric diet had reduced sclerotic glomerular numbers compared to those on normal diets (11.2±1 vs. 15.7±2, P<0.001), though glomerular numbers were similar in both groups.In summary, perinatal LP-HS diets significantly affected the BW, BP, renal injuries and kidney function of offspring. RR was seriously reduced, especially among offspring in hs-and perinatal LP groups. The most interesting result was the glomerular maturation staging in the pups, which suggests delayed nephrogenesis by a maternal LP diet.

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A New Method for Continuous Chronic Measurement and Recording of Blood Pressure, Heart Rate and Activity in the Rat Via Radio-telemetry

Cited by 198 publications

References 5 publications

Renoprotective effects of vasopeptidase inhibition in an experimental model of diabetic nephropathy

Renoprotective effects of vasopeptidase inhibition in an experimental model of diabetic nephropathy

Telemetry for Cardiovascular Monitoring in a Pharmacological Study

Effects of Perinatal Protein-Nacl Diets on Offspring's Blood Pressure and Renal Function in Lewis Rats

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