A New Medium for the Bacteriologic Examination of Stools (Lsu‐agar)

Juhlin, I; Ericson, Carl

doi:10.1111/j.1699-0463.1961.tb03192.x

Cited by 30 publications

(4 citation statements)

References 8 publications

(3 reference statements)

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“…Stool samples were collected from some of the symptomatic patients at Røros and Holtålen municipal health centres. Culture and biochemical identification of bacterial enteropathogens were done according to standard microbiological methods at St. Olavs Hospital in Trondheim [ 14 , 15 ]. The specimens were cultured for Salmonella, Shigella and Yersinia.…”

Section: Methodsmentioning

confidence: 99%

A large waterborne outbreak of campylobacteriosis in Norway: The need to focus on distribution system safety

et al. 2008

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Background: On 7 May 2007 the medical officer in Røros (population 5600) reported 15 patients with gastroenteritis. Three days later he estimated hundreds being ill. Untreated tap water from a groundwater source was suspected as the vehicle and chlorination was started 11 May. Campylobacter was isolated from patients' stool samples. We conducted an investigation to identify the source and describe the extent of the outbreak.

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Section: Methodsmentioning

confidence: 99%

A large waterborne outbreak of campylobacteriosis in Norway: The need to focus on distribution system safety

et al. 2008

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“…In the following procedure all incubations were performed at 37" C for 18-22 hours unless otherwise stated: Homogenized faeces were plated onto lactose bromthymol-blue agar (Drigalski agar), desoxycholate citrate agar (without sucrose) and LSU agar (10). Enrichment was performed in selenite broth and tetrathionate broth (Kaufmann-Muller) .…”

Section: I Processing Collected Materialsmentioning

confidence: 99%

Yersinia Enterocolitica in Small Rodents From Norway, Sweden and Finland

Kapperud

1975

Acta Pathologica Microbiologica Scandinavica Section B Microbio

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The purpose of the present investigation was to study the occurrence of Yersinia enterocolitica (Y.e.) among small rodents and shrews (Soricidae) from Norway, Sweden and Finand. During a period of one year beginning in the autumn of 1973, animals from seven localities were examined. Twenty‐four strains of Y.e. were isolated from 551 small rodents. Isolations were made from six of the seven localities and from six of the nine small rodent species examined. Pooled faeces samples from 397 animals yielded 12 strains. Faeces from 154 small rodents were separately investigated: Twelve (8 per cent) of these animals harboured Y.e. No isolations were made from 52 shrews. Most of the strains showed antigenic relationship to O‐serotypes previously reported from untreated drinking water in Norway. Two strains were antigenically related to O‐serotype 3 but differed biochemically from the strains found in man and swine. The results indicate that Y.e. has a widespread distribution among small rodents in Norway, Sweden and Finland.

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“…Media commonly used for the isolation of Y . enterocolitica from clinical specimens consist of peptones, or meat infusions supplemented with lactose and different agents promoting selectivity, such as sodium desoxycholate, potassium tetrathionate, bile salts, brilliant green and cristal violet (6,17,19).…”

Section: Discussionmentioning

confidence: 99%

AN EFFECTIVE, SELECTIVE MEDIUM FOR YERSINIA ENTEROCOLITICA CONTAINING SODIUM OXALATE

Soltész

Schalén

Mårdh

1980

Acta Pathologica Microbiologica Scandinavica Section B Microbio

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Soltkz, L. V., Schalen, C. & MBrdh, P.-A. An effective, selective medium for Yersinia enterocolitica containing sodium oxalate. Acta path. microbiol. scand. Sect. B, 88: 11-16, 1980. A medium O)Y(c medium) is described, which was more efficient for the isolation of Yersinia enterocolitica from experimentally infected faecal specimens than desoxycholate-citrate, McConkey , lactose-sucrose-urea (LSU) agar, and Yersinia selective medium (Wauter's medium). The DY(C medium consists of casein hydrolysate and peptone serving as carbon and energy sources. A high selectivity is achieved by its contents of sodium oxalate and bile salts. The oxalate suppresses growth of gramnegative rods, including members of the family Ennterobacteriaceae and of Pseudomonas spp., while the bile salts inhibit growth of gram-positive bacteria. In the few instances coliform rods grew on the VYCC medium, they could easily be distinguished by their fermentation of lactose, included in the medium, and the fact that colonies of organisms were surrounded by an opaque zone of precipitated bile salts. The most optimal condition for the isolation of Y. enterocolitica from stools was achieved at incubation of the ~Ytc medium at 29 O C for 2 days.

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A New Medium for the Bacteriologic Examination of Stools (Lsu‐agar)

Cited by 30 publications

References 8 publications

A large waterborne outbreak of campylobacteriosis in Norway: The need to focus on distribution system safety

A large waterborne outbreak of campylobacteriosis in Norway: The need to focus on distribution system safety

Yersinia Enterocolitica in Small Rodents From Norway, Sweden and Finland

AN EFFECTIVE, SELECTIVE MEDIUM FOR YERSINIA ENTEROCOLITICA CONTAINING SODIUM OXALATE

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