A New Medical Device Rigeneracons Allows to Obtain Viable Micro‐Grafts From Mechanical Disaggregation of Human Tissues

Abstract

Autologous graft is considered the gold standard of graft materials; however, this approach is still limited due to both small amount of tissue that can be collected and to reduced cell viability of cells that can be obtained. The aim of this preliminary study was to demonstrate the efficacy of an innovative medical device called Rigeneracons® (CE certified Class I) to provide autologous micro-grafts immediately available to be used in the clinical practice. Moreover, Rigeneracons® is an instrument able to create micro-grafts enriched of progenitors cells which maintain their regenerative and differentiation potential. We reported preliminary data about viability cell of samples derived from different kind of human tissues, such as periosteum, cardiac atrial appendage biopsy, and lateral rectus muscle of eyeball and disaggregated by Rigeneracons®. In all cases we observed that micro-grafts obtained by Rigeneracons® displayed high cell viability. Furthermore, by cell characterization of periosteum samples, we also evidenced an high positivity to mesenchymal cell markers, suggesting an optimal regenerative potential.

Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation

“…This evidence is in agreement with other recent papers in which the efficacy of this protocol in vitro was tested also in other type of tissues, including periosteum, biopsy of cardiac atria and lateral rectus muscle of eyeball 5 . In particular, we observed in Ded and dermis samples an increased cell viability after 7 days of culture, probably due to the use of a medium supplemented with 10% fetal bovine serum.…”

“…This protocol allows us not only to obtain viable but also autologous micro-grafts, given that the donor subject is also the acceptor of these micro-grafts. The capacity of this system to obtain autologous micro-grafts was especially reported in the field of dentistry where it has been shown that transplantation of autologous Dental Pulp Stem Cells (DPSCs) in a non-contained infrabony defect contributed to periodontal repair and regeneration of atrophic maxilla 5,6 . The ability of these micro-grafts to improve the wound healing process was also previously reported in the management of complex wounds after surgical interventions or complications 4 .…”

“…A case presentation is also herein described as a clinical application of autologous micro-grafts obtained by this protocol in combination with collagen sponges. This approach has already been reported to be efficient in the mechanical disaggregation of human tissues 5 and it has been used clinically for grafts and regeneration of dermal tissues 6,7 as well as for regenerative therapies of connective tissues in oralmaxillofacial surgery [8][9][10] .…”

Nanofeatured Titanium Surfaces for Dental Implantology: Biological Effects, Biocompatibility, and Safety

“…Some techniques allow for the correction of bone defects by grafting adult mesenchymal cells [160]. The use of nanofeatured surfaces, in association with these bone regeneration techniques, could allow for better new-bone formation and ultimately improve osseointegration and implant success.…”

The role of adipose stem cells in inflammatory bowel disease: From biology to novel therapeutic strategies

“…Nevertheless, some investigators have devised a new clinical process of tissue regeneration, using an enzymatic 92 and a mechanical disaggregation. 93 The novel strategy permits a mechanical disaggregation of the tissue without manipulating the matrix. The mechanical isolating procedure is easier to use, safer, less expensive and more rapid proving it more advantageous compared to the current enzymatic procedures in use.…”