A new mechanism in plant engineering: The potential roles of microRNAs in molecular breeding for crop improvement

Liu, Qing; Chen, Yue‐Qin

doi:10.1016/j.biotechadv.2010.01.002

Cited by 72 publications

(35 citation statements)

References 80 publications

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“…miRNAs are important regulators of many physiological and developmental processes (Voinnet, 2009), and their misexpression leads to a variety of defects in plants (Wu, 2013). Various studies have demonstrated that miRNAs regulate flowering, leaf, seed, and root morphology, as well as stress and hormonal responses in plants (Leung and Sharp, 2010;Liu and Chen, 2010;Stauffer and Maizel, 2014;Hong and Jackson, 2015;Zhang, 2015). Recent developments in genome-wide experimental approaches involving miRNA overexpression (Wu, 2013), use of miRNA target mimics (Franco-Zorrilla et al, 2007;Eamens and Wang, 2011;Meng et al, 2012), and detailed analysis of miRNA promoters (Baek et al, 2013;Sharma et al, 2015) were helpful to understand the physical interactions between miRNAs and their target mRNAs as well as their biological significance in plant growth and development (de Lima et al, 2012;Wu, 2013).…”

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confidence: 99%

MicroRNA858 Is a Potential Regulator of Phenylpropanoid Pathway and Plant Development

et al. 2016

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MicroRNAs (miRNAs) are endogenous, noncoding small RNAs that function as critical regulators of gene expression. In plants, miRNAs have shown their potential as regulators of growth, development, signal transduction, and stress tolerance. Although the miRNA-mediated regulation of several processes is known, the involvement of miRNAs in regulating secondary plant product biosynthesis is poorly understood. In this study, we functionally characterized Arabidopsis (Arabidopsis thaliana) miR858a, which putatively targets R2R3-MYB transcription factors involved in flavonoid biosynthesis. Overexpression of miR858a in Arabidopsis led to the down-regulation of several MYB transcription factors regulating flavonoid biosynthesis. In contrast to the robust growth and early flowering of miR858OX plants, reduction of plant growth and delayed flowering were observed in Arabidopsis transgenic lines expressing an artificial miRNA target mimic (MIM858). Genome-wide expression analysis using transgenic lines suggested that miR858a targets a number of regulatory factors that modulate the expression of downstream genes involved in plant development and hormonal and stress responses. Furthermore, higher expression of MYBs in MIM858 lines leads to redirection of the metabolic flux towards the synthesis of flavonoids at the cost of lignin synthesis. Altogether, our study has established the potential role of light-regulated miR858a in flavonoid biosynthesis and plant growth and development.

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confidence: 99%

MicroRNA858 Is a Potential Regulator of Phenylpropanoid Pathway and Plant Development

et al. 2016

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“…miR159 has also been found to regulate fl owering time and anther development through targeting of GAMYB (an activator of LY, an important factor in fl oral development). Increased levels of miR159 cause a reduction in the level of LFY transcripts, delay fl owering and perturb anther development (see Liu and Chen 2010 and references therein).…”

Section: Flowering Locus C ( Flc ) Plays a Central Role In Repressingmentioning

confidence: 99%

“…Similarly, transformed rice plants overexpressing transcription factors from the APETALA group, such as AP37 and AP59, under the constitutive promoter OsCc1 showed enhanced resistance to considerable drought and salinity stresses. Additionally, miRNAs also respond to drought, cold, salinity and oxidative stress (see Liu and Chen 2010 and references therein).…”

Section: Disease Resistance and Tolerance To Stressmentioning

confidence: 99%

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Towards the Domestication of Jatropha: The Integration of Sciences

Carels

2012

Jatropha, Challenges for a New Energy Crop

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N. Carels approximately 170 known species. Recent phylogenetic studies in the genus Jatropha based on random ampli fi ed polymorphic DNA (RAPD), ampli fi ed fragment length polymorphisms (AFLP, Vos et al. 1995 ) and nuclear ribosomal-DNA internal transcribed spacer (nrDNA ITS) markers (Sudheer et al. 2009a ) showed that J. curcas is genetically more similar to J. integerrima Jacq. than to any other species of genus Jatropha .J. curcas is a stem-succulent deciduous tree of ~5 m in height that produces seeds rich in oil (27-40%). Crude oil of J. curcas meets the fuel quality standards of rapeseed and can be easily converted into biodiesel that meets US and European standards by transesteri fi cation.J. curcas is currently considered as undomesticated; its physiology and agronomy remain to be clari fi ed, and thus seed yield is poorly predictable (Ginwal et al. 2004 ) . However, in the interval of just 3 years from 2007 to 2010, great efforts have been made by the scienti fi c community, particularly in India, to gather data on the genetic diversity and interspeci fi c hybridization of this species, as well as from analyses using the complete range of available biotechnologies, including in vitro propagation, tissue culture, genetic transformation, transcriptome description and genome sequencing. Forward and reverse genomics approaches are now beginning to be employed in J. curcas research.The twenty-fi rst century is witnessing an unprecedented level of scienti fi c integration, which in the case of biology, has led to the concept of systems biology . This concept can be de fi ned as the study of the interactions between the components of biological systems and how these interactions give rise to the functioning and behavior of that system. In the case of genetics, the system also includes the environment of the biological entity under consideration because it conditions the spectrum of stimuli that are used to score the genome performance. As a consequence, systems biology implies high-throughput technologies of data collection, information technology for data management and mathematical modeling.Genetic correlations can initially be investigated by examining domesticated crops as an entity that represents a particular mode of the expression of a plant species. Related to this notion, there is a common suite of traits known as the "domestication syndrome" (Doebley et al. 2006 ;Tang et al. 2010 ) , which represents the kind of complexity that is appropriate to the new perspective that plant breeding must be addressed with the support of systems biology.Here, I propose to discuss the current knowledge and emerging technologies available from model crops with the underlying prospect of their application to the selective breeding of J. curcas . Mating SystemJ. curcas is monoecious, with separate male and female fl owers, and is a predominantly outbreeding species, as shown by its high abortion rate, low seed-ovule ratio, low fecundity rate, high pollinator attractiveness, delayed stigma receptive

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“…Increasing world population, the drastic climate change, the threat of biotic stress and abiotic stress factors on the plants and the scarcity of arable lands have brought major concerns about the food security and its sustainability in the future; therefore, new agricultural technologies should be developed to prevent the worst-case scenario in the future [1]. With the advent of the next-generation sequencing (NGS) technologies, complex genomes of organisms can be unraveled [2].…”

Section: Introductionmentioning

confidence: 99%

In silico Analysis of MicroRNAs in Spinacia oleracea Genome and Transcriptome

Avşar¹,

Aliabadi²

2017

IJBBB

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Plant microRNAs (miRNAs) are small non-coding RNAs, about 21-24 nucleotides, which have important regulatory roles in growth, development, metabolic and defense processes. These critical elements regulate pathways either by inducing translational repression or messenger RNA (mRNA) decay. With the advent of the next-generation sequencing technologies and newly developed bioinformatics tools, the identification of microRNA studies by computational methods have been increased. Thus, the sequencing information provides us information for mining some known and unknown miRNAs in plants.In this study, we predict 34 putative miRNAs from Spinacia oleracea genome and two putative miRNA families from spinach transcriptome by using homology-based conservation method. RepeatMasker program is utilized to mask and eliminate five miRNA families out of 34 putative miRNA families from spinach genome. Finally, we analyze the targets of putatively identified miRNAs and their representation of genes (the copy number of each miRNA) throughout the genome.

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A new mechanism in plant engineering: The potential roles of microRNAs in molecular breeding for crop improvement

Cited by 72 publications

References 80 publications

MicroRNA858 Is a Potential Regulator of Phenylpropanoid Pathway and Plant Development

MicroRNA858 Is a Potential Regulator of Phenylpropanoid Pathway and Plant Development

Towards the Domestication of Jatropha: The Integration of Sciences

In silico Analysis of MicroRNAs in Spinacia oleracea Genome and Transcriptome

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