Abstract:We have isolated a new matrix protein family (N16) which is specific to the nacreous layer of the Japanese pearl oyster, Pinctada fucata, and have cloned and characterized the cDNAs coding for the components. Analysis of the deduced amino acid sequence revealed that N16 showed no definitive homology with other proteins. The in vitro studies of the crystallization clarified that N16 induced aragonite crystals when fixed on the substrate but inhibited crystal formation without it. The aragonite crystals showed p… Show more
“…Because of the diversity of preparative extraction, data are confused. Moreover, soluble matrices have been extracted from the insoluble matrix using different etching processes such as alkali solution [24]. Such results confirm the TEM observations of Nakahara [13] on the structure of the interlamellar membranes of the nacreous structure.…”
Section: Comparison With the Known Proteins In Nacresupporting
Mollusk shells, especially the nacre, are of commercial interest as well as palaeoenvironmental proxies. They are also investigated as biomaterials for medical purposes and biomimetics. Although the mineralogy is well-known and unique (aragonite tablets), the organic components are various. However, determination of the precise composition of the soluble organic matrix (SOM) of the nacreous layer is difficult. Among the range of possible techniques, 1D electrophoresis and High-performance liquid chromatography (HPLC) have previously been applied separately to differentiate pI and molecular weights. To date, no clear correlation has been established between the two parameters obtained in such conditions. Here, we report the use of preparative electrophoresis, coupled with HPLC, to determine the molecular weights of the pI fractions. The results are compared with 2D gel electrophoresis. It is shown that both methods have drawbacks and advantages, and are not redundant. The complexity of the composition of the nacreous tablet shown by scanning electron microscope (SEM) and Atomic Force Microscope (AFM) observations is also evidenced by electrophoresis and HPLC.
“…Because of the diversity of preparative extraction, data are confused. Moreover, soluble matrices have been extracted from the insoluble matrix using different etching processes such as alkali solution [24]. Such results confirm the TEM observations of Nakahara [13] on the structure of the interlamellar membranes of the nacreous structure.…”
Section: Comparison With the Known Proteins In Nacresupporting
Mollusk shells, especially the nacre, are of commercial interest as well as palaeoenvironmental proxies. They are also investigated as biomaterials for medical purposes and biomimetics. Although the mineralogy is well-known and unique (aragonite tablets), the organic components are various. However, determination of the precise composition of the soluble organic matrix (SOM) of the nacreous layer is difficult. Among the range of possible techniques, 1D electrophoresis and High-performance liquid chromatography (HPLC) have previously been applied separately to differentiate pI and molecular weights. To date, no clear correlation has been established between the two parameters obtained in such conditions. Here, we report the use of preparative electrophoresis, coupled with HPLC, to determine the molecular weights of the pI fractions. The results are compared with 2D gel electrophoresis. It is shown that both methods have drawbacks and advantages, and are not redundant. The complexity of the composition of the nacreous tablet shown by scanning electron microscope (SEM) and Atomic Force Microscope (AFM) observations is also evidenced by electrophoresis and HPLC.
“…Also, the pearl extract is observed to act as a factor to growth and cell proliferation in the previous works [2,3]. In recent years, there have been several studies on the cloning of the shell organic matrix proteins [4][5][6]. The shell organic matrix proteins are composed of two kinds of CaCO 3 polymorphs of the primatic layer and the nacreous layer.…”
Abstract. Caused by acute radiation skin reaction and injury, receiving radiotherapy treatment process is often performed side-effects on cancer patients. The clinical manifestations of skin irritation, itching, peeling, pigmentation, ulcer bleeding and other symptoms, in addition to causing patient discomfort and affecting quality of life, may increase the risk of local or systemic infection, and lead to interruption of radiation therapy. At present, for acute radiation dermatitis, there is no uniform treatment, and the various methods are evaluated variously. In this study, the authors focus on broken pearls using room temperature super extraction system, the water extraction process of wet-grinding method, nano-scale pearl, along with a large number of high purity natural amino acid extracts in the water. The room-temperature super-extraction system (RTSES) can be extracted from a relatively high-volume of pearl extract. We use pearl extract as the main component of experimental material, and the blending of pearl extract and poly (Îł-glutamic acid) is used to form biodegradable composite hydrogels. This study aims to evaluate the use of RTSES to extract the major active components of pearl and enhance their antiinflammation and anti-apoptosis effects. The possible effect of pearl extract on inducing apoptosis in human keratinocyte cells (HaCaT) under the exposure of low dose UVB has been investigated. Various concentrations of pearl extracts have been used to study the effect of low dosage UVB on HaCaT cells. The results show that pearl extract has no toxic effect on HaCaT cells. Combining the pearl extract and poly (Îł-glutamic acid) hydrogels with UVB irradiation would decrease the inflammation and apoptosis of HaCaT cells. The commercial pearl extract has the potential to inhibit radiation dermatitis occurring within keratinocyte cells.
“…Dissolution of the crystal units releases various macromolecules into solution, and it is well known that organic components are key participants in the control processes of shape and structure in mineralized tissues (2,3,17,22). Only some proteins have been isolated from the aragonitic nacreous layers of Pinctada (23) since the first evidence of the presence of organic matrices (24). Thus, before the cloning of "pure" proteins of mollusk shell layers, a better knowledge of the bulk composition of their SOM is necessary as a first step in understanding the mechanisms of layer formation.…”
The calcitic prisms of the shells of two bivalves, Pinna and Pinctada, are considered simple prisms according to some morphological and mineralogical characteristics. Scanning electron microscopic and atomic force microscopic studies show that the microstructures and nanostructures of these two shells are different. Pinna prisms are monocrystalline, whereas Pinctada prisms are not. Moreover, intraprismatic membranes are present only in the Pinctada prisms. The soluble organic matrices extracted from these prisms are acidic, but their bulk compositions differ. Ultraviolet and infrared spectrometries, fluorescence, high pressure liquid chromatography, and electrophoresis show that the sugarprotein ratios and the molecular weights are different. Sulfur is mainly associated with acidic sulfated sugars, not with amino acids, and the role of acidic sulfated sugars is still underestimated. Thus, the simple prism concept is not a relevant model for the biomineralization processes in the calcitic prismatic layer of mollusk shells.
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