A new mast cell degranulating peptide "mastoparan" in the venom of Vespula lewisii.

Hirai, Yuko; Yasuhara, Tadashi; Yoshida, Hisanobu; Nakajima, Terumi; Fujino, Masahiko; Kitada, Chieko

doi:10.1248/cpb.27.1942

Cited by 275 publications

(155 citation statements)

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“…Mastoparan, a 14-amino acyl wasp venom peptide (Hirai et al, 1979), activates some heterotrimeric G-proteins by stimulating a GTP-for-GDP exchange on their o~-subunits (Higashijima et al, 1988(Higashijima et al, , 1990Weingarten et al, 1990). …”

Section: G-protein-activating Mastoparan Inhibits In Vitro Vacuole Fumentioning

confidence: 99%

G-protein ligands inhibit in vitro reactions of vacuole inheritance.

Haas

Conradt

Wickner

1994

The Journal of Cell Biology

166

209

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Abstract. During budding in Saccharomyces cerevisiae, maternal vacuole material is delivered into the growing daughter cell via tubular or vesicular structures. One of the late steps in vacuole inheritance is the fusion in the bud of vesicles derived from the maternal vacuole. This process has been reconstituted in vitro and requires isolated vacuoles, a physiological temperature, cytosolic factors, and ATP (Conradt, B., J. Shaw, T. Vida, S. Emr, and W. Wickner. 1992. J.Cell Biol. 119:1469-1479). We now report a simple and reliable assay to quantify vacuole-to-vacuole fusion in vitro. This assay is based on the maturation and activation of vacuole membrane-bound pro-alkaline phosphatase by vacuolar proteinase A after vacuole-tovacuole fusion. In vitro fusion allowed maturation of 30 to 60% of pro-alkaline phosphatase. Vacuoles prepared from a mutant defective in vacuole inheritance in vivo (vac2-1) were inactive in this assay. Vacuole fusion in vitro required a vacuole membrane potential. Inhibition by nonhydrolyzable guanosine derivatives, mastoparans, and benzalkonium chloride suggest that GTP-hydrolyzing G proteins may play a key role in the in vitro fusion events.

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Section: G-protein-activating Mastoparan Inhibits In Vitro Vacuole Fumentioning

confidence: 99%

G-protein ligands inhibit in vitro reactions of vacuole inheritance.

Haas

Conradt

Wickner

1994

The Journal of Cell Biology

166

209

View full text Add to dashboard Cite

show abstract

“…Mastoparans are tetradecapeptides isolated from wasp venom as reported in [57,58] and they are well known as heterotrimeric G-protein activators as described in [59,60]. Moreover, mastoparan is able to activate the NPR-GC-B associated with plasma membranes fractions from TSM in [56].…”

Section: Body: Cyclic Gmp Signals During Muscarinic Activationmentioning

confidence: 99%

Two Guanylylcyclases Regulate the Muscarinic Activation of Airway Smooth Muscle

Alfonzo¹,

Placeres-Uray²,

Borges³

et al. 2012

Current Basic and Pathological Approaches to the Function of Muscle Cells and Tissues - From Molecules to Humans

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“…By this means, we isolated and characterized chemically a series of new dodecapeptideamides, named mastoparan and mastoparan-X, from the venom of Vespula lewr's:'z' and Vespa xam'/roprera, respectively (1,2).…”

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confidence: 99%