A New lncRNA, <i>lnc-LLMA</i>, Regulates Lipid Metabolism in Pig Hepatocytes

Lin, Yongcheng; Tai, Lina; Gao, Jiayi; Sun, Meng; Liu, Siqi; Huang, Tengda; Yu, Jingsu; Zhang, Zhiwang; Miao, Weiwei; Li, Yixing; Song, Ziyi; Zhang, Haojie; Zhou, Lei

doi:10.1089/dna.2021.0220

Cited by 7 publications

(5 citation statements)

References 46 publications

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“…These results indicated that hepatocytes of Dahe black pigs exhibited more abundant metabolic and biosynthetic functions, especially in protein metabolism, lipid metabolism, amino acid metabolism, and carbohydrate metabolism. This is supported by a recent report demonstrating specificity in liver lipid metabolism between different pig breeds [ 73 ]. The findings highlight breed differences in liver tissue between Dahe pigs (a Chinese indigenous breed) and Dahe black pigs (a crossbreed using the Duroc × Dahe breeding scheme through five generations of selection).…”

Section: Discussionsupporting

confidence: 78%

Single-nucleus transcriptome sequencing reveals hepatic cell atlas in pigs

Zhu,

Guan,

et al. 2023

BMC Genomics

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Background As the largest substantive organ of animals, the liver plays an essential role in the physiological processes of digestive metabolism and immune defense. However, the cellular composition of the pig liver remains poorly understood. This investigation used single-nucleus RNA sequencing technology to identify cell types from liver tissues of pigs, providing a theoretical basis for further investigating liver cell types in pigs. Results The analysis revealed 13 cells clusters which were further identified 7 cell types including endothelial cells, T cells, hepatocytes, Kupffer cells, stellate cells, B cells, and cholangiocytes. The dominant cell types were endothelial cells, T cells and hepatocytes in the liver tissue of Dahe pigs and Dahe black pigs, which accounts for about 85.76% and 82.74%, respectively. The number of endothelial cells was higher in the liver tissue of Dahe pigs compared to Dahe black pigs, while the opposite tendency was observed for T cells. Moreover, functional enrichment analysis demonstrated that the differentially expressed genes in pig hepatic endothelial cells were significantly enriched in the protein processing in endoplasmic reticulum, MAPK signaling pathway, and FoxO signaling pathway. Functional enrichment analysis demonstrated that the differentially expressed genes in pig hepatic T cells were significantly enriched in the thyroid hormone signaling pathway, B cell receptor signaling pathway, and focal adhesion. Functional enrichment analysis demonstrated that the differentially expressed genes in pig hepatic hepatocytes were significantly enriched in the metabolic pathways. Conclusions In summary, this study provides a comprehensive cell atlas of porcine hepatic tissue. The number, gene expression level and functional characteristics of each cell type in pig liver tissue varied between breeds.

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Section: Discussionsupporting

confidence: 78%

Single-nucleus transcriptome sequencing reveals hepatic cell atlas in pigs

Zhu,

Guan,

et al. 2023

BMC Genomics

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“…In addition, the level of proteins involved in fat metabolism was directly and indirectly related to lncRNA 21 686 and miR-146b, respectively. Although a plethora of published evidence has clarified the role of lncRNAs in the regulation of hepatic lipid metabolism in mammals (Faramarzzadeh et al, 2023;Muret et al, 2019;Pradas-Juni et al, 2020;Yu et al, 2022), the regulation of hepatic lipid homeostasis by lncRNAs in chickens is a novel and unclear field. The efforts of Li et al by transcriptome profile analysis showed that the regulation of genes involved in fat metabolism in Gallus gallus was probably through both lncRNAs and miRNAs, which may interact with steroid hormones (Li et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

LncRNAENSGALG00000021686 regulates fat metabolism in chicken hepatocytes via miR‐146b/AGPAT2 pathway

Xing,

2024

Animal Genetics

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The liver contributes to lipid metabolism as the hub of fat synthesis. Long non‐coding RNAs (lncRNAs) are considered the regulators of cellular processes. Since LncRNA ENSGALG00000021686 (lncRNA 21 686) has been described as a regulator of lipid metabolism, the present study aimed to clarify the role of lncRNA 21 686 in chicken hepatocytes’ lipid metabolism. Thirty‐two chickens were divided into four groups and were treated with diets containing different amounts of fat, and the hepatic expression of lncRNA 21 686 and miR‐146b along with the levels of proteins involved in the regulation of fat metabolism, lipid indices and oxidative stress were measured. Moreover, primary chicken hepatocytes were transfected with lncRNA 21 686 small interfering RNA or microRNA (miRNA, miR)‐146b mimics to measure the consequences of suppressing lncRNA or inducing miRNA expression on the levels of proteins involved in fat metabolism and stress markers. The results showed that the high‐fat diet modulated the expression of lncRNA 21 686 and miR‐146b (p‐value < 0.001). Moreover, there was a significant increase in 1‐acyl‐sn‐glycerol‐3‐phosphate acyltransferase 2 (AGPAT2) gene expression and protein levels and modulated fat‐related markers. Furthermore, the results showed that lncRNA 21 686 suppression reduced the expression of AGPAT2 and its downstream proteins (p‐value < 0.05). Overexpression of miR‐146b regulated fat metabolism indicator expression. Transfection experiments revealed that lncRNA 21 686 suppression increased miR‐146b expression. The findings suggested a novel mechanism containing lncRNA 21 686/miR‐146b/AGPAT2 in the regulation of fat metabolism in chicken hepatocytes.

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“…Duroc is a classic lean pig breed, with marked differences in backfat thickness and fat deposition compared with Luchuan pig [ 14 ]. Both breeds are good models for investigating the regulatory mechanism of fat metabolism [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

The landscape of super-enhancer regulates remote target gene transcription through loop domains in adipose tissue of pig

Yu,

Huang,

Liu

et al. 2024

Heliyon

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A New lncRNA, lnc-LLMA, Regulates Lipid Metabolism in Pig Hepatocytes

Cited by 7 publications

References 46 publications

Single-nucleus transcriptome sequencing reveals hepatic cell atlas in pigs

Single-nucleus transcriptome sequencing reveals hepatic cell atlas in pigs

LncRNAENSGALG00000021686 regulates fat metabolism in chicken hepatocytes via miR‐146b/AGPAT2 pathway

The landscape of super-enhancer regulates remote target gene transcription through loop domains in adipose tissue of pig

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