A new liver graft preparation method for uncontrolled non-heart-beating donors, combining short oxygenated warm perfusion and prostaglandin E1

Hara, Yasuyuki; Akamatsu, Yorihiro; Maida, Kai; Kashiwadate, Toshiaki; Kobayashi, Yoshinobu; Ohuchi, Noriaki; Satoh, Susumu

doi:10.1016/j.jss.2013.04.030

Cited by 31 publications

(14 citation statements)

References 32 publications

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“…hypothermic, subnormothermic and normothermic), we noted marked inconsistency in the actual temperatures denoted by these terms (see Table ). Despite including a description of the technique of MP, a number of papers failed to specify what particular temperatures were used in their respective studies while some descriptions used arbitrary and unspecific terms such as “warm,” “cold,” or “room temperature” to denote the temperatures used during MP. Of the 58 studies on hypothermic MP, 26 (45%) reported perfusing the livers at 4°C, whereas the rest performed MP at different temperatures within the 0°C–10°C range.…”

Section: Resultsmentioning

confidence: 99%

Machine Perfusion of Donor Livers for Transplantation: A Proposal for Standardized Nomenclature and Reporting Guidelines

Karangwa

Dutkowski

Fontes

et al. 2016

American Journal of Transplantation

132

113

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With increasing demand for donor organs for transplantation, machine perfusion (MP) promises to be a beneficial alternative preservation method for donor livers, particularly those considered to be of suboptimal quality, also known as extended criteria donor livers. Over the last decade, numerous studies researching MP of donor livers have been published and incredible advances have been made in both experimental and clinical research in this area. With numerous research groups working on MP, various techniques are being explored, often applying different nomenclature. The objective of this review is to catalog the differences observed in the nomenclature used in the current literature to denote various MP techniques and the manner in which methodology is reported. From this analysis, we propose a standardization of nomenclature on liver MP to maximize consistency and to enable reliable comparison and meta‐analyses of studies. In addition, we propose a standardized set of guidelines for reporting the methodology of future studies on liver MP that will facilitate comparison as well as clinical implementation of liver MP procedures.

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Section: Resultsmentioning

confidence: 99%

Machine Perfusion of Donor Livers for Transplantation: A Proposal for Standardized Nomenclature and Reporting Guidelines

Karangwa

Dutkowski

Fontes

et al. 2016

American Journal of Transplantation

132

113

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“…Dynamic preservation has emerged as a potential solution to the risk of greater I/R injury in DCD livers, the result of donor warm ischemia time. Several studies have reported that oxygenated perfusion before static CS reduces I/R damage in DCD organs [ 17 , 18 ]. In this study, OW increased the ATP/ADP tissue ratio and reduced liver enzyme release in DCD livers as documented by our analysis before the preservation period.…”

Section: Discussionmentioning

confidence: 99%

Liver Graft Susceptibility during Static Cold Storage and Dynamic Machine Perfusion: DCD versus Fatty Livers

Ferrigno

Pasqua

Berardo

et al. 2017

IJMS

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We compared static preservation (cold storage, CS, 4 °C) with dynamic preservation (machine perfusion, MP, 20 °C) followed by reperfusion using marginal livers: a model of donation after cardiac death (DCD) livers and two models of fatty livers, the methionine-choline deficient (MCD) diet model, and obese Zucker (fa/fa) rats. CS injury in DCD livers was reversed by an oxygenated washout (OW): hepatic damage, bile flow, and the ATP/ADP ratio in the OW + CS group was comparable with the ratio obtained with MP. Using fatty livers, CS preservation induced a marked release in hepatic and biliary enzymes in obese Zucker rats when compared with the MCD group. The same trend occurred for bile flow. No difference was found when comparing MP in MCD and obese Zucker rats. Fatty acid analysis demonstrated that the total saturated (SFA)/polyunsaturated fatty acid (PUFA) ratio was, respectively, 1.5 and 0.71 in obese Zucker and MCD rats. While preservation damage in DCD livers is associated with the ATP/ADP recovered with OW, injury in fatty livers is linked to fatty acid constituents: livers from obese. Zucker rats, with greater content in saturated FA, might be more prone to CS injury. On the contrary, MCD livers with elevated PUFA content might be less susceptible to hypothermia.

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“…Previous research has identified the ability of PGE 1 to improve myocardial microcirculation, reduce ischemicreperfusion injury, and exert anti-inflammatory and antiapoptotic effects on the myocardium ( 10 – 13 , 31 ). PGE 1 also exhibits general cytoprotective effects and anti-apoptotic activity ( 32 ). It has previously been reported that PGE 1 is able to significantly upregulate antiapoptotic proteins, such as Bcl-2 ( 33 ); and downregulate Bax and caspase-3 ( 13 ).…”

Section: Discussionmentioning

confidence: 99%

Prostaglandin E1 protects bone marrow-derived mesenchymal stem cells against serum deprivation-induced apoptosis

Zeng

Deng

Jiang

et al. 2015

Molecular Medicine Reports

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Mesenchymal stem cells (MSCs) have become a recent focus of experimental and clinical research regarding myocardial regeneration. However, the therapeutic potential of these cells is limited by poor survival. Prostaglandin E1 (PGE1) is known to have anti-inflammatory and anti-apoptotic effects on the myocardium. The aim of the present study was to determine whether PGE1 could protect MSCs against serum deprivation (SD)-induced apoptosis. An SD model was used to induce apoptosis in MSCs in vitro. Apoptotic morphological changes were detected by Hoechst 33258 fluorescent nuclear staining; and Annexin V-fluorescein isothiocyanate/propidium iodide (PI) double staining and flow cytometry was used to quantify the rate of apoptosis. Western blot analysis was used to detect the expression levels of the apoptosis-associated proteins Bcl-2, Bax and caspase-3. The results of the present study demonstrated that SD induced apoptosis of MSCs, and that treatment with PGE1 attenuated the morphological changes characteristic of apoptosis. Annexin V/PI staining showed that the rate of apoptosis gradually increased with the duration of ischemia. Furthermore, treatment with PGE1 significantly reduced SD-induced apoptosis, decreased the protein expression levels of Bax and caspase-3, and increased the expression levels of Bcl-2. These data suggest that PGE1 is able to influence the survival of MSCs under certain conditions. These results may aid in improving the therapeutic efficacy of MSC transplantation used to treat chronic ischemic heart disease.

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A new liver graft preparation method for uncontrolled non-heart-beating donors, combining short oxygenated warm perfusion and prostaglandin E1

Cited by 31 publications

References 32 publications

Machine Perfusion of Donor Livers for Transplantation: A Proposal for Standardized Nomenclature and Reporting Guidelines

Machine Perfusion of Donor Livers for Transplantation: A Proposal for Standardized Nomenclature and Reporting Guidelines

Liver Graft Susceptibility during Static Cold Storage and Dynamic Machine Perfusion: DCD versus Fatty Livers

Prostaglandin E1 protects bone marrow-derived mesenchymal stem cells against serum deprivation-induced apoptosis

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