A new lipopeptide biosurfactant produced by Arthrobacter sp. strain MIS38

Morikawa, Masaaki; Daido, H.; Takao, Toshifumi; Murata, Shigeo; Shimonishi, Yasutsugu; Imanaka, Tadayuki

doi:10.1128/jb.175.20.6459-6466.1993

Cited by 318 publications

(195 citation statements)

References 23 publications

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“…In addition, surfactin was found to lower the surface tension of 0.1 M NaHCO 3 from 71.6 to 27.0 millinewton/m. An even better surface activity was found for arthrofactin, a lipopeptide biosurfactant isolated from Arthrobacter species strain MIS38 (31). The minimum surface tension of arthrofactin was 24 millinewton/m above the critical micelle concentration.…”

Section: Discussionmentioning

confidence: 84%

A New Glycosylated Lipopeptide Incorporated into the Cell Wall of a Smooth Variant of Gordona hydrophobica

Moormann

Zähringer

Moll

et al. 1997

Journal of Biological Chemistry

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A cell wall component of a smooth variant of Gordona hydrophobica 1775/15 was isolated and purified, and its structure was determined by various chemical methods, including chemical synthesis of part structures, Edman degradation, gas chromatography/mass spectrometry analysis, matrix-assisted laser desorption ionizationpost-source decay (MALDI-PSD) tandem mass spectrometry, and 1 H and 13 C NMR using one-and two-dimensional, homo-and heteronuclear correlated spectroscopy. The cell wall component was found to be a (mono-) glycosylated peptidolipid (GPL) consisting of a tridecapeptide interlinked by a ␤-hydroxylated fatty acid (3-hydroxyeicosanoic acid, 20:0 (3-OH)) to form a cyclic lactone ring structure. The main fraction of GPL, for which we propose the name gordonin, was identified as In contrast, rough variants of G. hydrophobica 1775/15 lack these peptidolipids or synthesize them to a much lesser extent indicating that gordonin contributes significantly to the physicochemical character of the cell surface.Biofiltration is the method of choice for the treatment of waste gases with high volumes and low concentrations of organic pollutants. This technology requires low investment and operation costs and guarantees high reliability. Although biofiltration is already widely used, knowledge about the biology underlying this technology is lacking. Only a few studies have so far been conducted on the microorganisms involved in the process of exhaust-air treatment. Recently, Bendinger et al.(1) isolated coryneform bacteria from a biofilter that was loaded with the waste gas from an animal-rendering plant. On the basis of chemotaxonomic differentiation they were able to identify strains of the genus Corynebacterium, Gordona, Mycobacterium, and Arthrobacter. Some biofilter isolates of Gordona hydrophobica (2) showed colony morphologies, which gave rise to different physicochemical properties of their cell surface. The rough variants possess an extremely hydrophobic cell surface due to a hydrophobic mycolic acid layer. In contrast, the smooth variants exhibit a hydrophilic to moderate hydrophobic surface (3), although they are also surrounded by a hydrophobic mycolic acid layer. Obviously, the smooth ones incorporate into their cell wall an additional surface component, which exposes its hydrophilic part toward the medium. The observation that rough colonies of Gordona only gave rise to rough colonies whereas smooth colonies gave rise to smooth as well as rough ones when grown on complex media indicated that the rough variants lost the ability to cover their extremely hydrophobic surface with a more hydrophilic compound.A similar change in colony morphology was reported for mycobacterial species that are closely related to the genus Gordona. Camphausen et al. (4) found a trehalose containing lipooligosaccharide to be responsible for the observed change in Mycobacterium paratuberculosis. Fregnan et al. (5) showed that smooth variants of an unclassified scotochromogenic Mycobacterium possessed a mycoside D that is absen...

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Section: Discussionmentioning

confidence: 84%

A New Glycosylated Lipopeptide Incorporated into the Cell Wall of a Smooth Variant of Gordona hydrophobica

Moormann

Zähringer

Moll

et al. 1997

Journal of Biological Chemistry

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“…The surface tension of the culture supernatant was measured by using a ring tensiometer (K 6 , Kruss, Germany). Oil displacement test was performed by determining the oil displacement area as described by Morikawa et al (1993). Rhamnolipid production was determined as the amount of rhamnose by a specific colorimetric method (Dische and Shettles, 1948).…”

Section: Methodsmentioning

confidence: 99%

Biosurfactant production by Pseudomonas aeruginosa A41 using palm oil as carbon source

Thaniyavarn

Chongchin

Wanitsuksombut

et al. 2006

J. Gen. Appl. Microbiol.

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“…D-serine is found frequently in the bacterial cell wall, lipopeptides and siderophores and can also exist as a free molecule (Morikawa et al, 1993;Demange et al, 1990;Bernardini et al, 1996;Kaiser & Benner, 2008). In marine environments, D-serine has been detected in dissolved organic matter (McCarthy et al, 1998;Kawasaki & Benner, 2006;Dittmar et al, 2001;Kaiser & Benner, 2008 (Kaiser & Benner, 2008), have the potential to produce D-serine.…”

Section: Summary and Perspectivesmentioning

confidence: 99%

Distribution of eukaryotic serine racemases in the bacterial domain and characterization of a representative protein in Roseobacter litoralis Och 149

et al. 2016

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Two distinct bacterial and eukaryotic serine racemases (SRs) have been identified based on phylogenetic and biochemical characteristics. Although some reports have suggested that marine heterotrophic bacteria have the potential to produce D-serine, the gene encoding bacterial SRs is not found in those bacterial genomes. In this study, using in-depth genomic analysis, we found that eukaryotic SR homologues were distributed widely in various bacterial genomes. Additionally, we selected a eukaryotic SR homologue from a marine heterotrophic bacterium, Roseobacter litoralis Och 149 (RiSR), and constructed an RiSR gene expression system in Escherichia coli for studying the properties of the enzyme. Among the tested amino acids, the recombinant RiSR exhibited both racemization and dehydration activities only towards serine, similar to many eukaryotic SRs. Mg 2+ and MgATP enhanced both activities of RiSR, whereas EDTA abolished these enzymatic activities. The enzymatic properties and domain structure of RiSR were similar to those of eukaryotic SRs, particularly mammalian SRs. However, RiSR showed lower catalytic efficiency for L-serine dehydration (k cat /K m 50.094 min 21 mM 21 ) than those of eukaryotic SRs reported to date (k cat /K m 50.6-21 min 21 mM 21 ). In contrast, the catalytic efficiency for L-serine racemization of RiSR (k cat /K m 53.14 min 21 mM 21 ) was 34-fold higher than that of L-serine dehydration. These data suggested that RiSR primarily catalysed serine racemization rather than dehydration.

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A new lipopeptide biosurfactant produced by Arthrobacter sp. strain MIS38

Cited by 318 publications

References 23 publications

A New Glycosylated Lipopeptide Incorporated into the Cell Wall of a Smooth Variant of Gordona hydrophobica

A New Glycosylated Lipopeptide Incorporated into the Cell Wall of a Smooth Variant of Gordona hydrophobica

Biosurfactant production by Pseudomonas aeruginosa A41 using palm oil as carbon source

Distribution of eukaryotic serine racemases in the bacterial domain and characterization of a representative protein in Roseobacter litoralis Och 149

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