A new Lactobacillus plantarum strain, TN8, from the gastro intestinal tract of poultry induces high cytokine production

Salah, Riadh Ben; Trabelsi, Imen; Mansour, Riadh Ben; Lassoued, Saloua; Chouayekh, Hichem; Béjar, Samír

doi:10.1016/j.anaerobe.2012.05.001

Cited by 45 publications

(46 citation statements)

References 38 publications

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“…The induction of cytokines TNF-α and IL-10 by LAB strains was more or less similar to the other reports [29] [34]. Thus these isolates can be used as anti-inflammatory strains.…”

Section: Discussionsupporting

confidence: 68%

“…) resulting in bacteria-to-cell ratio 10:1 and co-incubated for 24 h in antibiotic and serum free RPMI-1640 medium at 37˚C in a 12-well tissue culture plates [29]. After incubation the supernatants were collected and used immediately for estimation of cytokines.…”

Section: Isolation Of Human Peripheral Blood Mononuclear Cells (Hpbmcmentioning

confidence: 99%

“…The anti-inflammatory cytokine IL-10 was also measured in the supernatant of hPBMCs. The assays for both the pro-inflammatory (TNF-α) and anti-inflammatory (IL-10) cytokines were performed twice independently in duplicates [29]. TNF-α and IL-10 cytokines were measured using anti-human TNF-α and anti-human IL-10 monoclonal antibodies respectively by sandwich ELISA kit (Diasource) as per manufacturer's recommendations.…”

Section: Isolation Of Human Peripheral Blood Mononuclear Cells (Hpbmcmentioning

confidence: 99%

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Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with <i>Piper betle</i> L. Leaves

Dubey¹,

Jeevaratnam²

2015

AiM

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The in vitro screening of lactobacilli isolates from uttapam batter, fermented supplementing with Piper betle L. leaves, was performed in order to select potent isolates for probiotic use. Their resistance to simulated gastric and intestinal juices as well as their adhesion to epithelial intestinal HCT-15 and vaginal HeLa cell lines were assessed and also evaluated for their immunomodulatory ability in ex vivo condition. Isolates were able to adhere as well as reduce pathogen adhesion to monolayer cell lines' surfaces and reduced production of pro-inflammatory cytokine TNF-α in LPS treated PBMCs, while enhanced that of anti-inflammatory cytokine IL-10. The isolates exhibited properties of auto-aggregation, co-aggregation, hydrophobicity, bile salt hydrolase activity and strong antimicrobial activity against pathogenic bacteria. Thus, the different lactobacilli isolates displayed potent probiotic and immunomodulatory properties among which AJ7 and AJ82 had a great potential and may have applications in fermented foods as immunomodulatory probiotic additives.

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“…The induction of cytokines TNF-α and IL-10 by LAB strains was more or less similar to the other reports [29] [34]. Thus these isolates can be used as anti-inflammatory strains.…”

Section: Discussionsupporting

confidence: 68%

Section: Isolation Of Human Peripheral Blood Mononuclear Cells (Hpbmcmentioning

confidence: 99%

Section: Isolation Of Human Peripheral Blood Mononuclear Cells (Hpbmcmentioning

confidence: 99%

See 1 more Smart Citation

Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with <i>Piper betle</i> L. Leaves

Dubey¹,

Jeevaratnam²

2015

AiM

View full text Add to dashboard Cite

show abstract

“…Stress adaptation mechanisms triggered by acidic environments can result in bile salt resistance (as shown in our findings), which can be unpredictable and higher than the resistance to acidic conditions (Sahadeva et al ., ). Other studies have also demonstrated that probiotic bacteria exhibit high tolerance to high bile salt levels (Kimoto‐Nira et al ., ; Ben Salah et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Potentially probiotic acetic acid bacteria isolation and identification from traditional dairies microbiota

Haghshenas

Nami

Abdullah

et al. 2014

Int J of Food Sci Tech

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Summary Among probiotics, acetic acid bacteria (AAB) can be used as preservative agents because of their high fermentation and acidification activities. This study aimed to isolate, identify and biologically characterise Acetobacter strains from traditional Iranian dairy products. Acetobacter strains were identified by catalase assay, Gram staining, and combined repetitive sequence‐based PCR [(GTG)5‐PCR fingerprints] and 16S rDNA gene sequencing. We identified eight strains belonging to four species, including Acetobacter aceti, Acetobacter indonesiensis, Acetobacter cibinongensis and Acetobacter syzygii. The molecular techniques could be used as an effective and rapid alternative tool to identify and characterise dairy‐associated AAB. Primary probiotic assessments, including low pH and high bile salt tolerance tests, antagonistic activity test against pathogens, and antibiotic susceptibility confirmed the probiotic properties of these AAB, particularly A. cibinongensis 34L strain, which was isolated from curd. Therefore, this strain can be introduced as novel candidate probiotics that could be used in the food industry.

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“…The ability of the isolate to grow at different temperature (range 4-70 • C), NaCl concentration (1-5%), and pH (2-8) was analyzed [18]. The probiotic potential of the isolate was verified by its acid and bile tolerance as described previously [19]. The 16S rRNA based genotype identification of the isolate was carried out as described earlier [16].…”

Section: Characterization Of Bacteriocinogenic-probiotic Isolate Bdu3mentioning

confidence: 99%

Bacteriocinogenic potential of a probiotic strain Bacillus coagulans [BDU3] from Ngari

Abdhul

Mohan

Shanmughapriya

et al. 2015

International Journal of Biological Macromolecules

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A new Lactobacillus plantarum strain, TN8, from the gastro intestinal tract of poultry induces high cytokine production

Cited by 45 publications

References 38 publications

Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with <i>Piper betle</i> L. Leaves

Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with <i>Piper betle</i> L. Leaves

Potentially probiotic acetic acid bacteria isolation and identification from traditional dairies microbiota

Bacteriocinogenic potential of a probiotic strain Bacillus coagulans [BDU3] from Ngari

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A new Lactobacillus plantarum strain, TN8, from the gastro intestinal tract of poultry induces high cytokine production

Cited by 45 publications

References 38 publications

Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with &lt;i&gt;Piper betle&lt;/i&gt; L. Leaves

Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with &lt;i&gt;Piper betle&lt;/i&gt; L. Leaves

Potentially probiotic acetic acid bacteria isolation and identification from traditional dairies microbiota

Bacteriocinogenic potential of a probiotic strain Bacillus coagulans [BDU3] from Ngari

Contact Info

Product

Resources

About

Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with <i>Piper betle</i> L. Leaves

Probiotic Screening of Lactobacilli Isolates from Uttapam Batter Fermented Supplementing with <i>Piper betle</i> L. Leaves