A New Kinetic and Thermodynamic Study of Spectrophotometric Method for Determination of Adrenaline in its Pharmaceutical Formulations

Abachi, Mouayed Q. Al; Hadi, Hind

doi:10.1007/s11094-014-1151-2

Cited by 6 publications

(3 citation statements)

References 14 publications

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“…So, quick, simplified and inexpensive detection techniques for simultaneous determination of epinephrine and theophylline in biological fluids are of great importance. Several analytical procedures are available for epinephrine and theophylline that include spectrophotometry, high-performance liquid chromatography, capillary electrophoresis, chemiluminescence, surface-enhanced Raman scattering, gas chromatography-mass spectrometry, as well as electrochemical methods [ 11-22 ].…”

Section: Introductionmentioning

confidence: 99%

An electrochemical sensor based on a modified glassy carbon electrode for detection of epinephrine in the presence of theophylline

Beitollahi,

Sarbandian

2024

ADMET DMPK

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Background and purpose: Neurotransmitters are chemical messengers that enhance and balance signals between cells and target cells in the body. They are vital to the body's ability to function. Epinephrine is one of the most essential catecholamine neurotransmitters with an important biological and pharmacological role in the mammalian central nervous system. Therefore, it is very important to develop sensitive, simple, and fast methods for the determination of this compound. Experimental approach: In the present work, a glassy carbon electrode (GCE) modified with the cerium oxide-zinc oxide (CeO2-ZnO) nanocomposite (CeO2-ZnO/GCE) was developed for the sensitive and quick detection of epinephrine. The CeO2-ZnO nanocomposite was prepared by hydrothermal method. Electrochemical methods such as voltammetry and chronoamperometry techniques were used to investigate the performance of the developed sensor. Key results: The resulting CeO2-ZnO/GCE showed a remarkable response towards the determination of epinephrine. The electrochemical sensor demonstrated a wide dynamic linear range from 0.1 to 900.0 μM for analysis of epinephrine. The LOD equalled 0.03 μM for epinephrine. In addition, the electrochemical sensor had good feasibility for concurrent detection of epinephrine and theophylline. Furthermore, experimental outputs indicated that the oxidation peaks of epinephrine and theophylline were separated by a 685 mV difference between the two peaks in PBS at a pH of 7.0. Also, an electrochemical sensor has been employed to analyse epinephrine in real samples (urine and epinephrine Injection). Conclusion: The good and acceptable analytical performance of the developed sensor can provide a promising tool for the analysis of epinephrine in real samples.

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Section: Introductionmentioning

confidence: 99%

An electrochemical sensor based on a modified glassy carbon electrode for detection of epinephrine in the presence of theophylline

Beitollahi,

Sarbandian

2024

ADMET DMPK

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“…The effect of dopamine lies in the vital areas of the brain, as it affects mood, sleep, study, focus, and learning, so its deficiency may lead to certain diseases, including; Parkinson's and depression [3][4]. Numerous analytical methods are used to determine adrenaline and dopamine, such as chromatographic methods [5][6][7][8], voltammetry [9][10][11][12][13][14], capillary electrophoresis [15], flow injection [16][17][18], ion-selective electrode [19][20][21][22][23], spectrophotometric [24][25][26][27][28][29][30] and spectrofluorimetric [31][32][33][34] methods, It is known that the fluorescence measurement methods are sensitive, so in this paper, an easy method was described to measure the fluorescence of adrenaline and dopamine and it does not require a lot of materials, that is, it is economically feasible in addition to being in an aqueous medium, while most of the methods used expensive organic solvents, which areharmful to the environment.…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric determination of adrenaline and dopamine .

Dhamra¹,

Al-Sabha²,

Al-Enizzi³

2022

JES

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A sensitive fluorometric method, with few steps and suitable for the daily routine, was made for examining adrenaline hydrochloride and dopamine hydrochloride. The reliance in this paper was on the nucleophilic substitution interaction of the mentioned drugs with 1,2-naphthoquinone sulfonate (NQS) in an aqueous pH 6 to give a fluorescent product with a maximum emission wave at ʎem 471 nm after being excited at a maximum excitation wave at ʎex 300 nm. The plots have complied within the range of 0.01-4.0, 0.01-2 µg/ml, and The detection limits (0.0062, 0.0027) and quantitation limits were (0.0207, 0.0091) µg/ml, for adrenaline and dopamine respectively. The accuracy (% recovery) was between (99.21% -100.72%) and the relative standard deviation (RSD%) is better than 0.95%. It was also found that the formed product was in a ratio of 1:2 reagent to the drug. The estimation of adrenaline and dopamine has been successfully tested on the injection, and it is in good agreement with its approved value and with that of the British Pharmacopoeia method.

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“…A differential UV spectrophotometric procedure has also been used for the determination of MTD in pharmaceutical formulations in the presence of germanium dioxide at 292 nm (Davidson, 1984). MTD has been determined in the visible region after reaction with 2,3,5-triphenyltetrazolium chloride (El-Rabbat, Omar, 1978), potassium bromate (Mohamed, Salem, 1984), vanillin (Salem, 1985, p-dimethylaminocinnamaldehyde (Walash, Abououf, Salem, 1985), molybdophosphoric acid in sulphuric acid medium (Issopoulos, 1989), Fe(III), o-phenanthroline (Issopoulos, 1990), ferric chloride (Zivanovic, Vasiljevic, Radulovic, 1991), neotetrazolium chloride (Issopoulos, Economou, 1993), metaperiodate (Nevado, Gallego, Laguna, 1995), barbituric acid (Aman et al, 1998), isoniazid in the presence of N-bromosuccinimide (Nagaraja et al, 1998), polyphenol oxidase enzyme (Vieira, Fatibello Filho, 1998), diazotized sulfanilamide in the presence of molybdate (Nagaraja, Vasantha, Sunitha, 2001a), semicarbazide hydrochloride in the presence of potassium persulfate (Nagaraja et al, 2001b), ammonium molybdate (Ribeiro et al, 2005a;Ribeiro, Pezza, Pezza, 2005b), 2,2-diphenyl-picrylhydrazyl (Matos, Silva, Ribeiro, 2012) and ferric chloride/nitroso-R-salt (Al Abachi, Hadi, 2013). However, most of these methods suffer from several disadvantages, such as long-waiting times or a heating step for reaction development, instability of the colored species, complex procedure, requirement for nonaqueous media, poor detection limit, or lack of previous application to pharmaceutical formulations.…”

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confidence: 99%

Development and validation of a simple spectrophotometric method for the determination of methyldopa in both bulk and marketed dosage formulations

Ribeiro¹,

Duarte²

2014

Braz. J. Pharm. Sci.

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A simple, precise, sensitive, rapid, specific and economical spectrophotometric method was developed to determine methyldopa (MTD) content in bulk and pharmaceutical dosage formulations. The proposed method was based on the formation of a colored product from the nitrosation reaction of MTD with sodium nitrite in an acid medium. The resultant nitroso derivative species reacts further with sodium hydroxide and is converted it into a more stable compound. This yellow nitrosation product exhibited an absorption maximum at 430 nm. Beer's Law was obeyed in a concentration range of 6.37 to 82.81 mg mL -1 MTD with an excellent coefficient of determination (R 2 = 0.9998). No interference was observed from common excipients in formulations. The results showed the method to be simple, accurate and readily applied for the determination of MTD in pure form and in pharmaceutical preparations. The analytical results obtained for these products using the proposed method are in agreement with those of the Brazilian Pharmacopoeia procedure at a 95% confidence level.Uniterms: Methyldopa/spectrophotometric determination. Pharmaceutical formulations/quality control. Spectrophotometry/qualitative analysis.Desenvolveu-se método espectrofotométrico simples, preciso, sensível, rápido, específico e econômico para a determinação do teor de metildopa (MTD) em matéria-prima e em formulações farmacêuticas. O método proposto baseia-se na formação de um produto colorido resultante da reação de nitrosação da MTD com nitrito de sódio em meio ácido. A espécie resultante (nitroso derivado) reage com hidróxido de sódio e é convertida a um composto mais estável de cor amarela. Este produto exibiu máximo de absorção a 430 nm. A lei de Beer foi obedecida na faixa de concentração de 6,37 a 82,81 mg mL -1 de MTD com excelente coeficiente de determinação (R 2 = 0,9998). Não se observou interferência de excipientes comumente encontrados em formulações farmacêuticas comerciais. Os resultados demonstraram que o método proposto apresenta simplicidade, excelentes precisão e exatidão e pode ser aplicado para a determinação de MTD na sua forma pura e em preparações farmacêuticas. Os resultados analíticos obtidos pelo método proposto estão de acordo com aqueles obtidos pelo método oficial descrito na Farmacopéia Brasileira, a um nível de confiança de 95%.Unitermos: Metildopa/determinação espectrofotométrica. Formulações farmacêuticas/controle de qualidade. Espectrofotometria/análise qualitativa. INTRODUCTIONMethyldopa (MTD), chemically known as a-methyl-3,4-dihydroxyphenylalanine (Figure 1), is a catechol derivative (catecholamine) widely used as an antihypertensive agent. MTD is a centrally acting alpha 2 -adrenoreceptor agonist, which reduces sympathetic tone and produces a fall in blood pressure (Hoffman, 2005).Several types of analytical procedures have been HO HO COOH NH 2 CH 3 FIGURE 1 -Chemical structure of methyldopa. P. R. S. Ribeiro, R. M. Duarte 574 employed for the analysis of catechol derivatives in pharmaceutical formulations and/or biological spe...

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A New Kinetic and Thermodynamic Study of Spectrophotometric Method for Determination of Adrenaline in its Pharmaceutical Formulations

Cited by 6 publications

References 14 publications

An electrochemical sensor based on a modified glassy carbon electrode for detection of epinephrine in the presence of theophylline

An electrochemical sensor based on a modified glassy carbon electrode for detection of epinephrine in the presence of theophylline

Spectrofluorimetric determination of adrenaline and dopamine .

Development and validation of a simple spectrophotometric method for the determination of methyldopa in both bulk and marketed dosage formulations

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