A New Key to Freshwater and Soil Gymnamoeba

Soil microorganisms decrease barley biomass uniformly across contrasting nitrogen availability

“…We quantified the copy number of bacterial 16S rRNA gene in the rhizosphere (i.e. the sand particles removed from the plant roots as described above) using quantitative PCR (qPCR The number of cultivable protists were estimated by a most probable number method, where we prepared eight replicated 1:3 dilution series (based on 5 g rhizosphere material in 100 ml Neff's amoebae saline [60]) in 96-well microtiter plates (Costar ® 3598, Corning, Vordingborg, Denmark) with 0.1 ml of 0.1 g L -1 tryptic soy broth (TSB) (#211825, Becton-Dickinson, Lyngby, Denmark) [61]. The plates were stored in the dark at 15 °C, and the wells were examined for the presence of flagellates, amoebae and ciliates after one and three weeks using an inverted microscope (200 x magnification) (Olympus CK X31).…”

Identification of new amoebae strains in rainbow trout (Oncorhynchus mykiss, Walbaum) farms affected by nodular gill disease (NGD) in Northeastern Italy

“…A portion of the second left gill arch from each animal was put in NNA Petri dishes free from inactivated bacteria for the cultivation of cultures. The primary isolates obtained on day 10 post-inoculation were transferred to 1.5% nutrient agar containing malt nutrient agar with malt and yeast extracts with a few drops of PAGE solution Most hyperplastic filaments have groups with more than five amoebae, free or encysted along the tissue surface (Page, 1988) and then subcultured in Petri dishes with regular daily observation of the cultures. These agar plate cultures were maintained in an incubator at 15°C.…”

Isolation of Naegleria spp. from a Brazilian Water Source

“…The daily microscopic examination of non-nutrient agar (NNA) plates allowed for the identification of amoeba growth in 100% of the sampling sites that were covered in this study. According the amoeba cell morphologic profile defined by Page'S Key [45], two life cycle stages, trophozoite and cyst, were observed ( Figure 2). Pathogens 2020, 9, x FOR PEER REVIEW 4 of 13 to 7.08, and temperatures ranged from 17.8 to 21.8 °C.…”

“…The daily microscopic examination of non-nutrient agar (NNA) plates allowed for the identification of amoeba growth in 100% of the sampling sites that were covered in this study. According the amoeba cell morphologic profile defined by Page'S Key [45], two life cycle stages, trophozoite and cyst, were observed ( Figure 2). The appearance of trophozoites and cystic forms was regularly inspected until the cultures reached 14 days outgrowth and the correspondence between culture findings ( Figure 2) and their respective species was addressed by the molecular approach, detailed next.…”

“…The cultures were examined daily for the presence of amoeba growth and the trophozoites identified were sub-cultured onto new NNA plates and their growth was inspected in an inverted Nikon Eclipse TS 100 microscope. To infer the taxonomic identity based on the Page s classification key, the photomicrographs were screened for amoeba-related morphological characters [45]. The trophozoites were harvested in 2 mL Page s saline solution (PAS) [58], centrifuged (800× g, 10 min), suspended in a freezing solution (9:1, heat inactivated fetal bovine serum:DMSO), and then stored in liquid nitrogen vapor.…”